Skip to main content
SpringerLink
Log in

Effects of vitamin D3 metabolites on bone cell calcium transport

  • Published:
Calcified Tissue Research Aims and scope Submit manuscript

Summary

The vitamin D3 metabolite, 25-hydroxycholecalciferol, at concentrations of 0.01 to 10.0 μg/ml, decreased calcium uptake by isolated bone cells. The effect occurred within 1 min after the simultaneous addition of metabolite and45Ca. Lactic acid and ATP production by the cells was not affected. 24(R), 25-dihydroxycholecalciferol produced a similar decrease in calcium uptake. Vitamin D3 had no effect at concentrations from 0.01 to 10.0 μg/ml. No effect of 1,25-dihydroxycholecalciferol on calcium uptake was observed with concentrations from 0.1 to 100 ng/ml and various preincubation periods extending to 2 h. None of the agents had any effect on calcium efflux. The effects of 25-hydroxycholecalciferol and 24(R), 25-dihydroxycholecalciferol on calcium uptake were not seen in isolated fetal rat skin cell preparations.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. DeLuca, H.F.: Mechanism of action and fate of vitamin D. Vitam. Horm.25, 315–367 (1967)

    PubMed  Google Scholar 

  2. Norman, A.W.: The mode of action of vitamin D. Biol. Rev. (Camb.)43, 97–137 (1968)

    Google Scholar 

  3. Trummel, C.L., Raisz, L.G., Blunt, J.W., DeLuca, H.F.: 25-Hydroxycholecalciferol: Stimulation of bone resorption in tissue culture. Science163, 1450–1451 (1969)

    PubMed  Google Scholar 

  4. Haussler, M.R., Rasmussen, H.: The metabolism of vitamin D3 in the chick, J. Biol. Chem.247, 2328–2335 (1972)

    PubMed  Google Scholar 

  5. Raisz, L.G., Trummel, C.L., Halick, M.F., DeLuca, H.F.: 1,25-Dihydroxycholecalciferol: A potent stimulator of bone resorption in tissue culture. Science175, 768–769 (1972)

    PubMed  Google Scholar 

  6. Tanaka, Y., DeLuca, H.F.: Inhibition of the metabolism of 25-hydroxycholecalciferol by actinomycin D and cycloheximide. Proc. Natl. Acad. Sci. U.S.A.68, 605–609 (1971)

    PubMed  Google Scholar 

  7. Wong, R., Myrtle, J., Tsai, H., Norman, A.: Studies on calciferol metabolism. V. The occurrence and biological activity of 1,25-dihydroxyvitamin D3 in bone. J. Biol. Chem.247, 5728–5735 (1972)

    PubMed  Google Scholar 

  8. Peacock, M., Taylor, G.A., Redel, J.: The action of two metabolites of vitamin D3: 25,26 dihydroxycholecalciferol (25,26(OH)2D3) and 24,25-dihydroxycholecalciferol (24,25(OH)2D3) on bone resorption. FEBS Lett.62, 248–250 (1976)

    PubMed  Google Scholar 

  9. Stern, P.H., DeLuca, H.F., Ikekawa, N.: Bone resorbing activities of 24-hydroxystereoisomers of 24-hydroxyvitamin D3 and 24,25-dihydroxyvitamin D3. Biochem. Biophys. Res. Commun.67, 965–971 (1975)

    PubMed  Google Scholar 

  10. Wong, G.: 1,25-Dihydroxycholecalciferol and parathormone: Effects on isolated osteoclast-like and osteoblast-like cells. Science197, 663–665 (1977)

    PubMed  Google Scholar 

  11. Dziak, R., Stern, P.H.: Calcium transport in isolated bone cells. III. Effects of parathyroid hormone and cyclic 3′,5′ AMP. Endocrinology97, 1281–1287 (1975)

    PubMed  Google Scholar 

  12. Dziak, R., Brand, J.S.: Calcium transport in isolated bone cells. I. Bone cell isolation procedures. J. Cell. Physiol.84, 75–84 (1974)

    PubMed  Google Scholar 

  13. Dziak, R., Brand, J.S.: Calcium transport in isolated bone cells. II. Calcium transport studies. J. Cell. Physiol.84, 85–96 (1974)

    PubMed  Google Scholar 

  14. Dziak, R., Stern, P.H.: Responses of fetal rat bone cells and bone organ cultures to the ionophore, A23187. Calcif. Tissue Res.22, 137–147 (1976)

    PubMed  Google Scholar 

  15. Stern, P.H., Trummel, C.L., Schnoes, H.K., DeLuca, H.F.: Bone resorbing activity of vitamin D metabolites and congeners in vitro: influence of hydroxyl substituents in the A ring. Endocrinology97, 1552–1558 (1975)

    PubMed  Google Scholar 

  16. Raisz, L.G.: Bone resorption in tissue culture: factors influencing the response to parathyroid hormone. J. Clin. Invest.44, 103–116 (1965)

    PubMed  Google Scholar 

  17. Stern, P.H.: Inhibition by steroids of parathyroid hormone-induced45Ca release from embryonic rat bone in vitro. J. Pharmacol. Exp. Ther.168, 211–217 (1969)

    PubMed  Google Scholar 

  18. Dziak, R., Stern, P.H.: Parathyromimetic effects of the ionophore, A23187, on bone cells and organ cultures. Biochem. Biophys. Res. Commun.65, 1343–1349 (1975)

    PubMed  Google Scholar 

  19. Mellow, A.M., Stern, P.H.: Specific binding of 25-hydroxyvitamin D3 in bone cell cytosol. Pharmacologist18, 233 (1976)

    Google Scholar 

  20. Haddad, J.G., Birge, S.: Widespread specific binding of 25-hydroxycholecalciferol in rat tissues. J. Biol. Chem.250, 299–303 (1975)

    PubMed  Google Scholar 

  21. Lawson, D.E.M., Charman, M., Wilson, P.W., Edelstein, S.: Some characteristics of new tissue-binding proteins for metabolites of vitamin D other than 1,25-dihydroxyvitamin D. Biochim. Biophys. Acta437, 403–415 (1976)

    PubMed  Google Scholar 

  22. Wezeman, F.: 25-Hydroxyvitamin D3: Autoradiographic evidence of sites of action in epiphyseal cartilage and bone. Science194, 1069–1071 (1976)

    PubMed  Google Scholar 

  23. Peck, W.A., Dowling, I.: Failure of 1,25 dihydroxycholecalciferol to modify cyclic AMP levels in parathyroid hormone-treated and untreated bone cells. Endo. Res. Commun.2, 157–166 (1976)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Oral Biology, School of Dentistry, State University of New York at Buffalo, 14226, Buffalo, New York, USA

    Rosemary Dziak

Authors
  1. Rosemary Dziak
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dziak, R. Effects of vitamin D3 metabolites on bone cell calcium transport. Calc. Tis Res. 26, 65–70 (1978). https://doi.org/10.1007/BF02013236

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02013236

Key words

Search

Navigation