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Characterization of collagen II fibrils containing biglycan and their effect as a coating on osteoblast adhesion and proliferation

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Abstract

Collagen has been used as a coating material for titanium-based implants for bone contact and as a component of scaffolds for bone tissue engineering. In general collagen type I has been used, however very little attention has been focussed on collagen type II. Collagen-based coatings and scaffolds have been enhanced by the incorporation of the glycosaminoglycan chondroitin sulphate (CS), however the proteglycan biglycan, which is found in bone and contains glycosaminoglycan chains consisting of CS, has not been used as a biomaterial component. The study had the following aims: firstly, five different collagen II preparations were compared with regard to their ability to bind CS and biglycan and the changes in fibril morphology thereby induced. Secondly, the effects of biglycan on the adhesion of primary rat osteoblasts (rO) as well as the proliferation of rO, primary human osteoblasts (hO) and the osteoblast-like cell line 7F2 were studied by culturing the cells on surfaces coated with collagen II fibrils containing biglycan. Fibrils of the collagen II preparation which bound the most biglycan were used to coat titanium surfaces. Bare titanium, titanium coated with collagen II fibrils and titanium coated with collagen II fibrils containing biglycan were compared. It was found that different collagen II preparations showed different affinities for CS and biglycan. In four of the five preparations tested, biglycan reduced fibril diameter, however the ability of a preparation to bind more biglycan did not appear to lead to a greater reduction in fibril diameter. Fibrils containing biglycan promoted the formation of focal adhesions by rO and significantly enhanced the proliferation of hO but not of rO or 7F2 cells. These results should encourage further investigation of biglycan as a component of collagen-based scaffolds and/or coatings.

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Acknowledgements

The authors thank the German Ministry for Education and Research (BMBF) for financial support and and M. Schuhmann, H. Zimmermann and Ch. Kupke for technical assistance.

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Authors and Affiliations

  1. Institute of Material Science, Max Bergmann Center of Biomaterials, Technische Universität Dresden, 01069, Dresden, Germany

    Timothy Douglas, Sascha Heinemann, Christiane Knieb, Susanne Bierbaum, Dieter Scharnweber & Hartmut Worch

  2. Center of Theoretical Medicine, Institute of Physiological Chemistry, Fiedlerstrasse 42, 01307, Dresden, Germany

    Ute Hempel & Carolin Mietrach

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  1. Timothy Douglas
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Correspondence to Timothy Douglas.

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Douglas, T., Heinemann, S., Hempel, U. et al. Characterization of collagen II fibrils containing biglycan and their effect as a coating on osteoblast adhesion and proliferation. J Mater Sci: Mater Med 19, 1653–1660 (2008). https://doi.org/10.1007/s10856-007-3250-z

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