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Fabrication of a PLGA-collagen peripheral nerve scaffold and investigation of its sustained release property in vitro

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Abstract

This study deals with the fabrication of a peripheral nerve scaffold prepared with poly (lactic acid -co- glycolic acid) [PLGA] and acellularized pigskin collagen micro particles and the investigation of its sustained release property in vitro. We took bovine serum albumin [BSA] as model drug to investigate the sustained-release property of the scaffold in vitro. The results showed the scaffold could release BSA steadily with a rate of 6.6 ng/d (r = 0.994) or so. In a 1-month test period, the accumulative release ratio of BSA from the scaffold was up to 43%, and the shape of the scaffold was still originally well kept. In addition, the scaffold outcome non-immunogenicity, good cell adhesion and biodegradability. The results indicated a scaffold constructed by this technique would be a potential implanting support with prolonged sustained release function, such as for the use of nerve scaffold.

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References

  1. R. V. WEBER and S. E. MACKINNON, Clin. Plast. Surg. 32 (2005) 605

    Article  Google Scholar 

  2. J. S. TARAS, V. NANAVATI and P. STEELMAN, J. Hand Ther. 18 (2005) 191

    Google Scholar 

  3. R. Y. KANNAN, H. J. SALACINSKI, P. E. M. BUTLER and A. M. SEIFALIAN, Biotechnol. Appl. Biochem. 41 (2005) 193

    Article  CAS  Google Scholar 

  4. C. E. SCHMIDT and J. B. LEACH, Annu. Rev. Biomed. Eng. 5 (2003) 293

    Article  CAS  Google Scholar 

  5. T. NAKAMURA, Y. INADA, S. FUKUDA, M. YOSHITANI, A. NAKADA, S. ITOI, S. KANEMARU, K. ENDO and Y. SHIMIZU, Brain Res. 1027 (2004) 18

    Article  CAS  Google Scholar 

  6. K. MATSUMOTO, K. OHNISHI, T. KIYOTANI, T. SEKINE, H. UEDA, T. NAKAMURA, K. ENDO and Y. SHIMIZU, Brain Res. 868 (2000) 315

    Article  CAS  Google Scholar 

  7. T. TOBA, T. NAKAMURA, Y. SHIMIZU, K. MATSUMOTO, K. OHNISHI, S. FUKUDA, M. YOSHITANI, H. UEDA, Y. HORI and K. ENDO, J. Biomed. Mater. Res. 58 (2000) 622

    Article  Google Scholar 

  8. A. HAGIWARA, S. NAKASHIMA, T. ITOH, C. SAKAKURA, E. OTSUJI, H. YAMAGISHI, S. OKAJIMA, K. KUSUZAKI, H. HASE, S. KUBO, J. SOH, T. MIKI, T. TOBA, T. NAKAMURA and Y. SHIMIZU, Gan To Kagaku Ryoho. 29 (2002) 2202

    Google Scholar 

  9. X. WANG, W. HU, Y. CAO, J. YAO, J. WU and X. GU, Brain 128 (2005) 1897

    Article  Google Scholar 

  10. Y. INADA, S. MORIMOTO, Y. TAKAKURA and T. NAKAMURA, Neurosurgery 55 (2005) 640

    Google Scholar 

  11. L. M. LEE, M. C. HUANG, T. Y. CHUANG, L. S. LEE, H. CHENG and I. H. LEE, Life Sciences 74 (2004) 1937

    Article  CAS  Google Scholar 

  12. K. IWAYA, K. MIZOI, A. TESSLER and Y. ITOH, Neurosurgery 44 (1999) 590

    Article  Google Scholar 

  13. M. S. RAMER, J. V. PRIESTLEY and S. B. MCMAHON, Nature 403 (2000) 312

    Article  CAS  Google Scholar 

  14. Z. SAHENK, H. N. NAGARAJA, B. S. MCCRACKEN, W. M. KING, M. L. FREIMER, J. M. CEDARBAUM and J. R. MENDELL, Neurology 65 (2005) 681

    Article  CAS  Google Scholar 

  15. C. DEISTER and C. E. SCHMIDT, J. Neural Eng. 3 (2006) 172

    Article  CAS  Google Scholar 

  16. A. K. KITAHARA, Y. SUZUKI, P. QI, Y. NISHIMURA, K. SUZUKI, T. KIYOTANI, Y. TAKIMOTO, T. NAKAMURA, Y. SHIMIZU and K. ENDO, J. Plast. Reconstr. Surg. Hand. Surg. 33 (1999) 187

    Article  CAS  Google Scholar 

  17. S. SAHOO, H. OUYANG, J. C. GOH, T. E. TAY, and S. L. TOH, Transactions -7th World Biomaterials Congress, (2004) p. 8

  18. R. YANG, T. CHEN, H. CHEN, and W. WANG, in Proceedings of SPIE-The International Society for Optical Engineering, v 5345, Microfluidics, BioMEMS, and Medical Microsystems II, (2004) p. 130

  19. J. PARK, M. MELHEM, MURAD, and D. PANKAJ, in “Advances in Bioengineering—Vol. 55”, (American Society of Mechanical Engineers, Bioengineering Division (Publication) BED, 2003) p. 11

  20. Y. DONG and S.-S. FENG, Biomaterials 26, (2005) 6068

    Article  CAS  Google Scholar 

  21. S. DUVVURI, K. G. JANORIA and A. K. MITRA, J. Control. Release 108 (2005) 282

    Article  CAS  Google Scholar 

  22. G. KEILHOFF, F. STANG, G. WOLF and H. FANSA, Biomaterials 24 (2003) 2779

    Article  CAS  Google Scholar 

  23. S. YOSHII and M. OKA, Brain Res. 888 (2001) 158

    Article  CAS  Google Scholar 

  24. M.R. AHMED, S. VAIRAMUTHU, M. SHAFIUZAMA, S. H. BASHA and R. JAYAKUMAR, Brain Res. 1046 (2005) 55

    Article  CAS  Google Scholar 

  25. F. STANG, H. FANSA, G. WOLF, M. REPPIN and G. KEILHOFF, Biomaterials 26 (2005) 3083

    Article  CAS  Google Scholar 

  26. F. KANG and J. SINGH, Int. J. Pharmaceut. 260 (2003) 149

    Article  CAS  Google Scholar 

  27. M. VAN DE WEERT and W. E. HENNINK, W. JISKOOT, Pharm. Res. 17, (2000) 1159

    Article  Google Scholar 

  28. G. JIANG, B. H. WOO, F. KANG, J. SINGH and P. P. DELUCA, J. Control. Release 79 (2002) 137

    Article  CAS  Google Scholar 

  29. M. YAMASAKI, H. YANO and K. AOKI, Int. J. Biol. Macromol. 12 (1990) 263

    Article  CAS  Google Scholar 

  30. K. KALLENBACH, R. G. LEYH, E. LEFIK, T. WALLES, M. WILHELMI, S. CEBOTARI, A. SCHMIEDL, A. HAVERICH and H. MERTSCHING, Biomaterials 25, (2004) 3613

    Article  CAS  Google Scholar 

  31. M. A.AKASHI, The Research Foundation for Microbial Disease of OSAKA University, (1999) p. 10

  32. GB/T 16886.10, Standard Press of China, (2005)

  33. S.-H. HSU, C.-J. CHANG, C.-M. TANG and F.-T. LIN, J. Biomat. Appl. 19 (2004) 163

    Article  CAS  Google Scholar 

  34. S. RAIMONDO, S. NICOLINO, P. TOS, B. BATTISTON, M. G. GIACOBINI-ROBECCHI, I. PERROTEAU and S. GEUNA, J. Comp. Neurol. 489 (2005) 249

    Article  Google Scholar 

  35. K. YU and J. D. KOCSIS, J. Neurophysiol. 94 (2005) 1519

    Article  Google Scholar 

  36. A. I. GRAVVANIS, A. LAVDAS, A. E. PAPALOIS, I. FRANCESCHINI, D. A. TSOUTSOS, M. DUBOIS-DALCQ, R. MATSAS and J. D. IOANNOVICH, Microsurgery 25 (2005) 423

    Article  Google Scholar 

  37. A. KAMIJO, S. KAMEI, A. SAIKAWA, Y. IGARI and Y. OGAWA, J. Control. Rel. 40 (1996) 269

    Article  CAS  Google Scholar 

  38. C. -J. CHANGA and S.-H. HSU, Biomaterials 27 (2006) 1035

    Article  Google Scholar 

  39. F. DELUSTRO, R. A. CONDELL, M. A. NGUYEN and J. M. MCPHERSON, J. Biomed. Mater. Res. 20 (1986) 109

    Article  CAS  Google Scholar 

  40. S. STIVASTAVA and D. G. STEPHEN, Biomaterials 11 (1990) 133

    Article  Google Scholar 

Download references

Acknowledgement

This work got the support of the National Basic Research Program of China (973 Grant No. 2005CB522603) and “111-Project” (College of bioengineering, Chongqing University, P.R. China)

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Authors and Affiliations

  1. College of Bioengineering, Chongqing University, Chongqing, 400044, P.R. China

    Bin Liu, Shao-Xi Cai, Kai-Wang Ma, Zhi-Ling Xu, Xiao-Zhen Dai & Li Yang

  2. College of Life Science, Southwest University, Chongqing, 400716, P.R. China

    Bin Liu

  3. Engineering Research Center of Bioreactor, Ministry of Education, JiLin Agricultural University, Changchun, 130118, P.R. China

    Bin Liu & Xiao-Kun Li

  4. China–USA International Collaborative Lab, Chongqing University and UCSD, Chongqing, 400044, P.R. China

    Zhi-Ling Xu, Li Yang & KL. Paul Sung

  5. Department of Burn, The First Affiliated Hospital of Wenzhou Medical College, Wenzhou, 325000, P.R. China

    Cai Lin

  6. Wound Healing and Cell Biology Laboratory, Institute of Basic Medical Science, PLA General Hospital, Postgraduate Medical College, Beijing, 100853, P.R. China

    Xiao-Bing Fu

  7. Department of Orthopaedics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093-0412, USA

    KL. Paul Sung

  8. Department of Bioengineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093-0412, USA

    KL. Paul Sung

  9. School of Pharmacy, Wenzhou Medical College, Wenzhou, 325027, P.R. China

    Xiao-Kun Li

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  1. Bin Liu
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  2. Shao-Xi Cai
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  7. Cai Lin
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  9. KL. Paul Sung
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  10. Xiao-Kun Li
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Correspondence to Xiao-Kun Li.

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Liu, B., Cai, SX., Ma, KW. et al. Fabrication of a PLGA-collagen peripheral nerve scaffold and investigation of its sustained release property in vitro. J Mater Sci: Mater Med 19, 1127–1132 (2008). https://doi.org/10.1007/s10856-007-3224-1

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  • DOI: https://doi.org/10.1007/s10856-007-3224-1

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