Skip to main content
SpringerLink
Log in

Controlled release of insulin from PLGA nanoparticles embedded within PVA hydrogels

  • Published:
Journal of Materials Science: Materials in Medicine Aims and scope Submit manuscript

Abstract

A simple and versatile delivery platform for peptide and protein based on physically cross-linked poly (vinyl alcohol) (PVA) hydrogels containing insulin-loaded poly (lactic-co-glycolic acid) (PLGA) nanoparticles was successfully fabricated. The particle morphology and size were characterized by SEM and laser light scattering method, respectively. Results showed that these particles had a mean diameter of 615 nm with a narrow size distribution and homogeneous particle production. The protein encapsulation efficiency was 72.6%. When insulin-loaded PLGA nanoparticles were administered intraperitoneally as a single dose (20 U/kg) to streptozotocin-induced diabetic mouse, blood glucose levels of these mice decreased and it could be sustained at such levels over 24 h. In vitro release further indicated that entrapment of the nanoparticles into the PVA hydrogels causes a reduction in both the release rate and the total amount of insulin released, which suggesting that PLGA nanoparticles entrapped into the PVA hydrogels showed more suitable controlled release kinetics for protein delivery.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig 1
Fig 2
Fig 3
Fig 4
Fig 5
Fig 6

Similar content being viewed by others

References

  1. G. DE ROSA, R. IOMMELLI and M. I. LA ROTONDA, J. Control. Release. 69 (2000) 283

    Article  CAS  Google Scholar 

  2. Y. P. LI, Y. Y. PEI, X. Y. ZHANG, et al J. Control. Release. 71(2001) 203

    Article  CAS  Google Scholar 

  3. V. R. SINHA and A. TREHAN, J. Control. Release. 90(2003) 261

    Article  CAS  Google Scholar 

  4. T. L. LOWE, H. TENHU and H. TYLLI, J. Appl. Polym. Sci. 73 (1999) 1031

    Article  CAS  Google Scholar 

  5. M. GRAZIACASCONE, Z. H. ZHU and F. BORSELLI, J. Mater. Sci: Mater. Electron. 13 (2002) 29

    Google Scholar 

  6. J. H. JUANG, W. S. BONNER, Y. J. OGAWA, ET AL., Transplantation 61 (1996) 1557

    Google Scholar 

  7. D. H. CHEN, J. C. LEU and T. C. HUANG, J. Chem. Technol. Biotechnol. 61 (1994) 351

    Article  CAS  Google Scholar 

  8. M. KAZUHIRO, N. ATSUHI, F. SHINICHI, ET AL., Pharmaceut. Res. 6 (1989) 338

    Google Scholar 

  9. F. CASTELLI, G. PITARRESI and G. GIAMMONA, Biomaterials 21 (2000) 821

    Article  CAS  Google Scholar 

  10. M. J. D. NUGENT, A. HANLEY, P. T. TOMKINS, ET AL., J. Mater. Sci.: Mater. Med. 16 (2005) 1149

  11. C. M. HASSAN, J. E. STEWART and N. A. PEPPAS, Eur. J. Pharmaceut. Biopharmaceut. 49 (2000) 161

    Article  CAS  Google Scholar 

  12. R. R. CAO, S. M. ZHANG, Q. M. LUO, ET AL., Key Eng. Mater. 288–289 (2005) 151

    Google Scholar 

  13. F. YOKOYAMA, I. MASADA, K. SHIMAMURA, ET AL., Colloid Polym. Sci. 264 (1986) 595

    Google Scholar 

  14. V. I. LOZINSKY, A. L. ZUBOV and E. F. TITOVA, Enzym. Microbial Technol. 18 (1996) 561

    Article  CAS  Google Scholar 

  15. J. K. LI, N. WANG and X. S. WU, J. Control. Release. 56 (1998) 117

    Article  CAS  Google Scholar 

  16. C. M. HASSAN, J. E. STEWART and N. A. PEPPAS, Eur. J. Pharmaceut. 49 (2000) 161

    Article  CAS  Google Scholar 

  17. M. TAKENAGA, Y. YAMAGUCHI, A. KITAGAWA, ET AL., J. Control. Release. 79 (2002) 81

    Google Scholar 

  18. H. MURAKAMI, M. KOBAYASHI, H. TAKEUCHI, ET AL., J. Control. Release. 67 (2000) 29

  19. R. LIN, L. S. NG and C. -H. WANG, Biomaterials 26 (2005) 4476

    Article  CAS  Google Scholar 

  20. C. FONSECA, S. SIMÕES and R. GASPAR, J. Control. Release. 83 (2002) 273

    Article  CAS  Google Scholar 

  21. T. MUSUMECI, C. A. VENTURA, I. GIANNONE, ET AL., Int. J. Pharmaceut. 325 (2006) 172

    Google Scholar 

  22. M. LIU, J. DONG, Y. YANG, ET AL., Eur. Polym. J. 41 (2005) 375

  23. I. GALESKA, T. -K. KIM, S. D. PATIL, ET AL., The AAPS J. 7 (2005) E231

Download references

Acknowledgements

The financial supports from the 863 item 2006AA032439 and the NSFC under the contract numbers 30570493, 30200284 and 30411240531 are gratefully acknowledged.

Author information

Authors and Affiliations

  1. Advanced Biomaterials and Tissue Engineering Center, Huazhong University of Science and Technology, Wuhan, 430074, China

    J. Liu, S. M. Zhang, P. P. Chen, L. Cheng, W. Zhou, W. X. Tang, Z. W. Chen & C. M. Ke

Authors
  1. J. Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. M. Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. P. P. Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. L. Cheng
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. W. Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. W. X. Tang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Z. W. Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. C. M. Ke
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to S. M. Zhang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Liu, J., Zhang, S.M., Chen, P.P. et al. Controlled release of insulin from PLGA nanoparticles embedded within PVA hydrogels. J Mater Sci: Mater Med 18, 2205–2210 (2007). https://doi.org/10.1007/s10856-007-3010-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10856-007-3010-0

Keywords

Search

Navigation