Skip to main content
SpringerLink
Log in

Synthesis and characterization of poly(L-lactic acid-co-ethylene oxide-co-aspartic acid) and its interaction with cells

  • Published:
Journal of Materials Science: Materials in Medicine Aims and scope Submit manuscript

Abstract

Multiblock terpolymer of poly(L-lactic acid)/ poly(ethylene oxide)/poly(L-aspartic acid), (PLLA/PEO/ PAsp) was synthesized by ring opening polymerization of β -benzyl L-aspartate N-carboxyanhydride, Asp(OBzl)-NCA with α-ω -hydroxy terminated triblock PLLA/PEO/PLLA copolymer.

The resulting multiblock terpolymer was characterized by several techniques including Fourier transform infrared spectroscopy and differential scanning calorimetry.1H nuclear magnetic resonance spectra indicated the molar ratio of PLLA/PEO/PAsp (OBzl) to be 86/10/4.

Thermal gravimetric analysis and environmental scanning electron microscopy data showed that PLLA/PEO/PAsp had crystalline and brittle structure. In order to improve its mechanical and physical properties, the terpolymer was blended with high molecular weight poly(L-lactic-co-glycolic acid) copolymer, PLGA(85/15) (Mw: 95000 gmol− 1) in 25/75 and 50/50 mole ratios.

The hydrolytical degradation properties of these polymers were studied. Degradation experiments were performed during a 48-day period in pH:7.4 phosphate-buffered saline (PBS) at 37∘C. The observed molecular weight losses were 91% and 67% for the 25/75 and 50/50 mixtures, respectively. In vitro attachment and growth of L929 mouse fibroblasts on these biopolymers were also investigated. Cell growth experiments indicated that the copolymer blend allowed the attachment and growth of cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. D. K. GILDING and A. M. REED, Polymer, 20 (1979), 1459.

    CAS  Google Scholar 

  2. F. E. KOHN, J. W. A. VAN DEN BERG, G. V. RIDDER, J. Appl. Polym. Sci. 29, 1984, 4265.

    Article  CAS  Google Scholar 

  3. D. J. MOONEY, G. ORGAN, J. P. VACANTI and R. LANGER, Cell Transplant. 3, 1994, 203.

    CAS  Google Scholar 

  4. B. S. KIM and D. J. MOONEY, Trends Biotechnol. 16, 1998, 224.

    Article  CAS  Google Scholar 

  5. D. J. MOONEY, S. PARK, P. M. KAUFMANN, K. SANO, K. MCNAMARA, J. P. VACANTI, R. LANGER, J. Biomed. Mater. Res. 29, 1995, 959.

    Article  CAS  Google Scholar 

  6. P. J. A. VELD, Z. R. SHEN, G. A. J. TAKENS, P. J. DIJKSTRA and J. FEIJEN, J. Polym. Sci. Part A: Polym. Chem. 32, 1994, 1063.

    Google Scholar 

  7. D. A. BARRERA, E. ZYLSTRA, P. T. LANSBURY, R. LANGER, Macromolecules 28, 1995, 425.

    Article  CAS  Google Scholar 

  8. J. S. HRKACH, J. OU, N. LOTAN, R. LANGER, Macromolecules 28, 1995, 4736.

    Article  CAS  Google Scholar 

  9. G. JOHN, S. TSUDA, M. MORITA, J. Polym. Sci. Part A: Polym. Chem. 35, 1997, 1901.

    CAS  Google Scholar 

  10. T. OUCHI, T. NOZAKI, A. ISHIKAWA, I. FUJIMOTO and Y. OHYA, J. Polym. Sci. Part A: Polym. Chem. 35, 1997, 377.

    Article  CAS  Google Scholar 

  11. E. B. LAVIK, J. S. HRKACH, N. LOTAN, R. NAZAROV and R. LANGER, J. Biomed. Mater. Res. 58, 2001, 291.

    Article  CAS  Google Scholar 

  12. S. JO, P. S. ENGEL and A. G. MIKOS, Polymer 41, 2000, 7595.

    Article  CAS  Google Scholar 

  13. H. SHINODA, Y. ASOU, A. SUETSUGU and K. TANAKA, Macromolecular Bioscience. 3, 2003, 34.

    Article  CAS  Google Scholar 

  14. W. H. DALY and D. POCHE, Tetrahedron Letters 29, 1988, 5859.

    Article  CAS  Google Scholar 

  15. J. S. HRKACH, J. OU, N. LOTAN and R. LANGER, Mat. Res. Soc. Symp. Proc. 394, 1995, 77.

  16. J. S. HRKACH, J. OU, N. LOTAN and R. LANGER, ACS Symposium Series 627, 1996, 93.

  17. N. KAYAMAN, O. KARAL-YILMAZ, K. BAYSAL and B. M. BAYSAL, Polymer 42, 2001, 4109.

    Google Scholar 

  18. Y. GUO, K. BAYSAL, B. KANG, L. J. YANG and J. R. WILLIAMSON, J. Biol. Chem. 273, 1998, 4027.

    Google Scholar 

  19. A. F. THUNEMANN, J. BEYERMANN and H. KUKULA, Macromolecules 33, 2000, 5906.

    Google Scholar 

  20. L. LU, S. J. PETER, M. D. LYMAN, H. L. LAI, S. M. LEITE, J. A. TAMADA, S. UYAMA, J. P. VACANTI, R. LANGER and A. G. MIKOS, Biomaterials. 21, 2000, 1837.

    CAS  Google Scholar 

  21. K. CAI, K. YAO, X. HOU, Y. WANG, Y. HOU, Z. YANG, X. LI and H. XIE, J. Biomed Mater Res. 62, 2002, 283.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. TUBITAK, Research Institute for Genetic Engineering and Biotechnology, P.O.Box 21, 41470, Gebze-Kocaeli, Turkey

    Okşan Karal-Yilmaz, Kemal Baysal & Bahattin M. Baysal

  2. Department of Chemistry, Marmara University, 81040, Göztepe-Istanbul, Turkey

    Nilhan Kayaman-Apohan

  3. Advanced Technologies Research and Development Center, Bogazici University, 34342, Bebek-Istanbul, Turkey

    Zülal Misirli

  4. Department of Chemical Engineering, Bogazici University, 34342, Bebek-Istanbul, Turkey

    Bahattin M. Baysal

Authors
  1. Okşan Karal-Yilmaz
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Nilhan Kayaman-Apohan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zülal Misirli
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kemal Baysal
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Bahattin M. Baysal
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Karal-Yilmaz, O., Kayaman-Apohan, N., Misirli, Z. et al. Synthesis and characterization of poly(L-lactic acid-co-ethylene oxide-co-aspartic acid) and its interaction with cells. J Mater Sci: Mater Med 17, 213–227 (2006). https://doi.org/10.1007/s10856-006-7307-1

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10856-006-7307-1

Keywords

Search

Navigation