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Bacterial adhesion to bisphosphonate coated hydroxyapatite


Staphylococcus aureus (S. aureus) is commonly associated with microbial infection of orthopaedic implants. Such infections often lead to osteomyelitis, which may result in failure of the implant due to localised bone destruction. Bacterial adhesion and subsequent colonisation of the device may occur as a consequence of contamination during surgery, or by seeding from a distant site through the blood circulation. Coating of the hydroxyapatite (HA) ceramic component of artificial hip joints with the bisphosphonates clodronate (C) and pamidronate (P) has been proposed as a means to minimise osteolysis and thereby prevent loosening of the implant. However, the effect of the bisphosphonate coating on bacterial adhesion to the HA materials must be determined before this approach can be implemented. In this study coated HA materials were incubated with the S. aureus and the number of adherent bacteria determined using the Modified Vortex Device (MVD) method. The number of bacteria adherent to the P coated HA material was significantly greater than that adherent to uncoated HA (60-fold increase) or to the C coated HA (90-fold increase). Therefore, even though earlier studies suggested that P bound to HA may improve osseointegration, the results presented would suggest that the use of this coating may be limited by the potential increased susceptibility of the coated device to infection.

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  1. 1.

    R. G. FLEMMING, C. C. CAPELI, S. L. COOPER and R. A. PROCTOR, Biomaterials 21 (2000) 273.

    Google Scholar 

  2. 2.

    P. VAUDAUX, H. YASUDA, M. I. VELAZCO, E. HUGGLER, I. RATTI, F. A. WALDVOGEL and D. P. LEW, J. Biomater. Appl. 5 (1990) 134.

    Google Scholar 

  3. 3.

    G. GEYER and A. SCHWARZKOPF, HNO 47 (1999) 25.

    Google Scholar 

  4. 4.

    C. L. SCHULTZ, M. R. PEZZUTTI, D. SILOR and R. WHITE, J. Ind. Microbiol. 15 (1995) 243.

    Google Scholar 

  5. 5.

    M. DELMI, P. VAUDAUX, D. P. LEW and H. VASEY, J. Orthop. Res. 12 (1994) 432.

    Google Scholar 

  6. 6.

    L. MONTANARO, C. R. ARCIOLA, L. BALDASSARRI and E. BORSETTI, Biomaterials. 20 (1999) 1945.

    Google Scholar 

  7. 7.

    S. P. NAIR, S. MEGHJI, M. WILSON, I. NUGENT, A. ROSS, A. ISMAEL, N. K. BHUDIA, M. HARRIS and B. HENDERSON, Br. J. Rheum. 36 (1997) 328.

    Google Scholar 

  8. 8.

    E. INGHAM and J FISHER, Proc. Instn. Mech. Engrs. 21 (2000) 214.

    Google Scholar 

  9. 9.

    P. M. BOONEKAMP, L. J. A. VAN DER WEE-PALS, M. M. L. VAN WIJK-VAN-LENNEP, C. W. THESING and O. L. M. BIJVOET, Bone and Mineral. 1 (1986) 27.

    Google Scholar 

  10. 10.

    A. CARANO, S. L. TEITELBAUM, J. D. KONSEK, P. H. SCHLESINGER and H. C. BLAIR, J. Clin. Invest. 85 (1990) 456.

    Google Scholar 

  11. 11.

    M. G. CECCHINI, R. FELIX, H. FLEISCH and P. H. COOPER, J. Bone Min. Res. 2 (1987) 135.

    Google Scholar 

  12. 12.

    D. E. HUGHES, M. MIAN, D. F. GUILLAND-CUMMING and R. G. G. RUSSELL, Drugs Exptl. Clin. Res. 2 (1991) 109.

    Google Scholar 

  13. 13.

    S. M. HOROWITZ, S. A. ALGAN and M. A. PURDON, J. Biomed. Mater. Res. 31 (1996) 91.

    Google Scholar 

  14. 14.

    C. W. G. M. LOWIK, G. VAN DER PLUIJM, L. J. A. VAN DER WEE-PALS, H. BLOYS VAN TRESLONG-DE GROOT and O. L. M. BIJVOET, J. Bone Min. Res. 3 (1988) 185.

    Google Scholar 

  15. 15.

    M. J. ROGERS, D. J. WATTS, R. GRAHAM and R. RUSSELL, Amer. Cancer Soc. 80 (1997) 1652.

    Google Scholar 

  16. 16.

    G. A. RODAN, Ann. Rev. Pharmacol. Toxicol. 38 (1998) 375.

    Google Scholar 

  17. 17.

    S. ADAMI and N. ZAMBERLAN, Drug Safety 14 (1996) 158.

    Google Scholar 

  18. 18.

    A. GANGULI, C. HENDERSON, S. T. MEIKLE, A. W. LLOYD, I. GOLDIE and M. H. GRANT, J. Mater. Sci.: Mater. Med. 13 (2002) 923.

    Google Scholar 

  19. 19.

    M. PAULSSON, M. KOBER, C. FREIJ-LARSSON, M. STOLLENWERK, B. WESSLEN and A. LJUNGH, Biomaterials 14 (1993) 845.

    Google Scholar 

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Correspondence to M. H. Grant.

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Ganguli, A., Steward, C., Butler, S.L. et al. Bacterial adhesion to bisphosphonate coated hydroxyapatite. J Mater Sci: Mater Med 16, 283–287 (2005).

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  • Staphylococcus Aureus
  • Hydroxyapatite
  • Bisphosphonate
  • Staphylococcus
  • Osteomyelitis