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Visualization analysis of glucose, lactate and cholesterol based on an electrochemiluminescent biosensor array

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Abstract

In this work, three specialized oxidases for glucose, lactate and cholesterol are absorbed on carbon microspheres (CSs), which can produce hydrogen peroxide during their substrate-specific enzymatic activity. Electrochemiluminescent (ECL) is generated by the electrochemical reaction between luminol and hydrogen peroxide on an ECL biosensor array, whose light signals are collected by a CCD camera. Under optimum conditions, calibration curves are constructed for glucose, lactate, cholesterol concentrations and ECL intensities. The detection limits of the different biosensors are found to be 12 μM for glucose, 3 μM for lactate and 19 μM for cholesterol. Furthermore, the resulting ECL biosensor array is expected to be an alternative analytical tool in clinical analysis because of its good stability, acceptable precision and reproducibility.

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Acknowledgements

This research is supported by Anhui Provincial Natural Science Foundation (Grants No. 2008085QB68), Natural Science Foundation of Anhui Provincial Department of Education (No. KJ2021A0521) and National Undergraduate Innovation and Entrepreneurship Training Program (Grants No. 202010373008, 202010373022 and 202110373006).

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Authors and Affiliations

  1. Key Laboratory of Green and Precise Synthetic Chemistry and Applications, Ministry of Education; School of Chemistry and Materials Science, Huaibei Normal University, Huaibei, 235000, Anhui, People’s Republic of China

    Jiajia Chen, Fengting Dai, Haotian Liu, Zhengguang He, Hui Gao & Gen Liu

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  1. Jiajia Chen
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  2. Fengting Dai
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  3. Haotian Liu
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  4. Zhengguang He
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  5. Hui Gao
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  6. Gen Liu
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Contributions

JC: Methodology, Investigation, Data Curation. FD & ZH: Conceptualization, Methodology, Writing-Original Draft. HL: Investigation. HG: Data Curation, Visualization. GL: Validation, Resources, Supervision.

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Correspondence to Gen Liu.

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Chen, J., Dai, F., Liu, H. et al. Visualization analysis of glucose, lactate and cholesterol based on an electrochemiluminescent biosensor array. J Mater Sci: Mater Electron 34, 415 (2023). https://doi.org/10.1007/s10854-023-09852-3

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