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Enzyme sensor for simultaneous determination of cholesterol, triglyceride and HDL cholesterol in serum

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Abstract

A three-electrode lipid biosensor that simultaneously measures the total cholesterol (CHO), triglyceride (TG), and HDL cholesterol (HDL-C) in standard serum has been developed. The lipid biosensor is designed for clinical use where production cost, low sample requirement, portability, stability, and speed are high priorities. The device design filters out blood cells and lipoproteins from the serum, where the target molecules are catalyzed by enzymes encapsulated in carboxymethyl cellulose (CMC) to produce electron mediators, potassium ferrocyanide. These electron mediators were subsequently detected by amperometric determination. The sensor exhibit high selectivity towards the targets and can measure the target lipids in 4 min with 10 µL of serum. Lastly, the device can be stored up to 18 months with a minimal decrease in catalytic efficiency.

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Data availability statement

The dataset generated during the current study are not publicly available but are available from the corresponding author on reasonable request.

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Acknowledgements

We are grateful to Dr. Shunsuke Fujii from AIST for his support and technical input on our project.

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Authors and Affiliations

  1. Engineering Planning Office, Techno Medica Co., Ltd, Yokohama, Kanagawa, Japan

    Takeshi Uemura & Hiroki Yamazaki

  2. National Institute of Advanced Industrial Science and Technology (AIST), Sendai, Japan

    Tetsuji Itoh

  3. Department of Chemistry, Graduate School of Science, Tohoku University, Sendai, Japan

    Takeshi Uemura & Seiichi Nishizawa

Authors
  1. Takeshi Uemura
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  2. Hiroki Yamazaki
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  3. Tetsuji Itoh
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  4. Seiichi Nishizawa
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Corresponding authors

Correspondence to Takeshi Uemura, Tetsuji Itoh or Seiichi Nishizawa.

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On behalf of all authors, the corresponding author states that there is no conflict of interest.

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Uemura, T., Yamazaki, H., Itoh, T. et al. Enzyme sensor for simultaneous determination of cholesterol, triglyceride and HDL cholesterol in serum. ANAL. SCI. 38, 1189–1197 (2022). https://doi.org/10.1007/s44211-022-00148-w

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  • DOI: https://doi.org/10.1007/s44211-022-00148-w

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