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A temperature-sensitive hydrogel loaded with taxifolin promotes skin repair by modulating MAPK-mediated autophagic pathway

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Abstract

Today, skin wound healing is still a huge challenge, and how to effectively repair wounds has become a pressing issue. Hydrogel is a very promising treatment. In this study, we prepared a temperature-sensitive hydrogel by loading the active factor taxifolin (TAX) into poloxamer 407, chitosan (CS), and hyaluronic acid (HA). Scanning electron microscopy, X diffraction, and infrared spectroscopy revealed that taxifolin was successfully loaded in the temperature-sensitive hydrogel (TAX-gel). The cross-linked hydrogels exhibited better thermal stability, antioxidant activity, slow release, and safety. In addition, taxifolin composite hydrogel could accelerate the repair of skin damage in mice, and the analysis of the mechanism may be related to the activation of MAPK-mediated autophagy and the increase in VEGF, Pan-Keratin, and CD31 protein expression levels. In this experiment, by constructing a taxifolin temperature-sensitive hydrogel and revealing its mechanism of accelerating skin repair, we fill the gap of taxifolin temperature-sensitive hydrogel and lay the foundation for the development of clinical medical dressing products.

Graphical abstract

TAX-gel accelerates skin wound repair in mice.

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Data availability

The data that support the findings of this study are not openly available and are available from the corresponding author upon reasonable request.

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Acknowledgements

This study was supported by grants from the Jilin Province Science and Technology Development Program (Grant No. 20220508062RC) and the Jilin Agricultural Science and Technology College Doctoral starting fund [Grant No. (2022) 738].

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Author notes

  1. Chuanbo Ding and Ting Zhao have contributed equally to this work.

Authors and Affiliations

  1. College of Traditional Chinese Medicine, Jilin Agriculture Science and Technology College, Jilin, 132101, China

    Chuanbo Ding, Ziyang Liu, Ting Zhao, Xinglong Liu, Lina Ma & Min Yang

  2. Jilin Aodong Pharmaceutical Group Co. Ltd, Dunhua, China

    Chuanbo Ding

  3. College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, 130118, China

    Shuwen Sun & Jinping Zhang

Authors
  1. Chuanbo Ding
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Contributions

All authors made equal contributions to researching data, discussion, and analysis of the content of the manuscript. CD contributed to writing—original draft, visualization, data curation, investigation, funding acquisition. ZL contributed to visualization. TZ contributed to visualization, methodology, investigation, data curation, formal analysis. XL contributed to writing—review & editing. LM contributed to supervision. JZ contributed to supervision. MY contributed to data curation, investigation. SS contributed to conceptualization, funding acquisition, supervision, writing—review & editing.

Corresponding author

Correspondence to Shuwen Sun.

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Conflict of interest

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Ethical approval

This study was approved by the animal research ethics committee of Jilin Agricultural University, ethics approval No.: 2022-08-28-002.

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Ding, C., Liu, Z., Zhao, T. et al. A temperature-sensitive hydrogel loaded with taxifolin promotes skin repair by modulating MAPK-mediated autophagic pathway. J Mater Sci 58, 14831–14845 (2023). https://doi.org/10.1007/s10853-023-08951-0

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  • DOI: https://doi.org/10.1007/s10853-023-08951-0

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