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Synthesis of Ag@GQD and their application in photoacoustic imaging and chemical/photothermal combination therapy and bacteriostasis

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Abstract

Bacterial infections are closely associated with tumor growth and metastasis. Bacteriostasis can not only inhibit the growth and metastasis of tumors during their early diagnosis and treatment but also prevent bacterial infections after recovery. In this study, we synthesized Ag@GQD which consisted of nitrogen-doped graphene quantum dots (N-GQD) wrapped around Ag NPs through the photocatalytic reduction method. Multifunctional nanoprobes (Ag/DOX@GQD) were obtained by doxorubicin (DOX) loading. Ag@GQD exhibited high stability, low cytotoxicity, and good photoacoustic imaging performance. The effective photothermal performance of Ag@GQD enabled us to achieve the controlled release of DOX from probes using near-infrared light and pH response during the treatment of tumors using chemo-photothermal combination therapy. Results from cell-based experiments revealed that combination therapy was superior to single-drug therapy. Besides, Ag@GQD could effectively kill Staphylococcus aureus and Escherichia coli and can be expected to inhibit the growth and metastasis of tumors caused by bacteria and in reducing bacterial infections after recovery.

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References

  1. Zhu TH, Bao XX, Chen MY et al (2020) Mechanisms and future of non-small cell lung cancer metastasis. Front Oncol 10:2441

    Google Scholar 

  2. Gu Y, Lai ST, Dong Y et al (2021) AZD9291 resistance reversal activity of a pH-sensitive nanocarrier dual-loaded with chloroquine and FGFR1 inhibitor in NSCLC. Adv Sci 8:2002922

    Article  CAS  Google Scholar 

  3. Ma YF, Bai YA, Mao HL et al (2016) A panel of promoter methylation markers for invasive and noninvasive early detection of NSCLC using a quantum dots-based FRET approach. Biosens Bioelectron 85:641–648

    Article  CAS  Google Scholar 

  4. Li Z, Fu QR, Ye JM et al (2020) Ag+-coupled black phosphorus vesicles with emerging NIR-II photoacoustic imaging performance for cancer immune-dynamic therapy and fast wound healing. Angew Chem Int Edit 59:22202–22209

    Article  CAS  Google Scholar 

  5. Kim T, Zhang QZ, Li J et al (2018) A gold/silver hybrid nanoparticle for treatment and photoacoustic imaging of bacterial infection. ACS Nano 12:5615–5625

    Article  CAS  Google Scholar 

  6. Liu TW, Li X, Wang JL et al (2020) Ag@S-nitrosothiol core-shell nanoparticles for chemo and photothermal synergistic tumor targeted therapy. J Mater Chem B 8:5483–5490

    Article  CAS  Google Scholar 

  7. Attia ABE, Balasundaram G, Moothanchery M et al (2019) A review of clinical photoacoustic imaging: current and future trends. Photoacoustics 16:100144

    Article  Google Scholar 

  8. Rao AP, Bokde N, Sinha S et al (2020) Photoacoustic imaging for management of breast cancer: a literature review and future perspectives. Appl Sci-Basel 10:767

    Article  CAS  Google Scholar 

  9. Liu S, Pan X, Liu H (2020) Two-dimensional nanomaterials for photothermal therapy. Angew Chem Int Edit 59:5890–5900

    Article  CAS  Google Scholar 

  10. Xuan Y, Guan M, Zhang SB (2021) Tumor immunotherapy and multi-mode therapies mediated by medical imaging of nanoprobes. Theranostics 11:7360–7378

    Article  CAS  Google Scholar 

  11. Tang HX, Liu CG, Zhang JT et al (2020) Biodegradable quantum composites for synergistic photothermal therapy and copper-enhanced chemotherapy. ACS Appl Mater Inter 12:47289–47298

    Article  CAS  Google Scholar 

  12. Chow SC, Gowing SD, Cools-Lartigue JJ et al (2015) Gram negative bacteria increase non-small cell lung cancer metastasis via toll-like receptor 4 activation and mitogen-activated protein kinase phosphorylation. Int J Cancer 136:1341–1350

    Article  CAS  Google Scholar 

  13. Sarihan S, Ercan I, Saran A et al (2015) Evaluation of infections in non-small cell lung cancer patients treated with radiotherapy. Cancer Detect Prev 29:181–188

    Article  Google Scholar 

  14. Ye M, Gu X, Han Y et al (2016) Gram-negative bacteria facilitate tumor outgrowth and metastasis by promoting lipid synthesis in lung cancer patients. J Thorac Dis 8:1943–1955

    Article  Google Scholar 

  15. Mann M, Chowdhury B, Sullivan E et al (2003) Serious asthma exacerbations in asthmatics treated with high-dose formoterol. Chest 124:70–74

    Article  CAS  Google Scholar 

  16. An J, Li Z, Dong Y et al (2016) Methicillin-resistant staphylococcus aureus infection exacerbates NSCLC cell metastasis by up-regulating TLR4/MyD88 pathway. Cell Mol Biol 62:1–7

    CAS  Google Scholar 

  17. Wang ZP, Fang YS, Zhou XF et al (2020) Embedding ultrasmall Ag nanoclusters in luria-bertani extract via light irradiation for enhanced antibacterial activity. Nano Res 13:203–208

    Article  CAS  Google Scholar 

  18. Sharma VK, Yngard RA, Lin Y et al (2009) Silver nanoparticles: green synthesis and their antimicrobial activities. Adv Coll Interfac 145:83–96

    Article  CAS  Google Scholar 

  19. Han XW, Meng XZ, Zhang J et al (2016) Ultrafast synthesis of silver nanoparticle decorated graphene oxide by a rotating packed bed reactor. Ind Eng Chem Res 55:11622–11630

    Article  CAS  Google Scholar 

  20. Han XW, Bi SD, Zhang WQ et al (2019) One-step fabrication of highly dispersed ag nanoparticles decorated n-doped reduced graphene oxide heterogeneous nanostructure for the catalytic reduction of 4-nitrophenol. Coll Surf A 574:69–77

    Article  CAS  Google Scholar 

  21. Xiang LA, Sl B, Qiang BA et al (2020) Gold nanoclusters decorated amine-functionalized graphene oxide nanosheets for capture, oxidative stress, and photothermal destruction of bacteria. Coll Surf B: Biointerfaces 196:111313

    Article  Google Scholar 

  22. Xuan Y, Zhang RY, Zhang XS et al (2018) Targeting N-doped graphene quantum dot with high photothermal conversion efficiency for dual-mode imaging and therapy in Vitro. Nanotechnology 29:355101

    Article  Google Scholar 

  23. Wu C, Yuan YL, He Q et al (2016) Two-step synthesis of Ag@GQD hybrid with enhanced photothermal effect and catalytic performance. Nanotechnology. https://doi.org/10.1088/0957-4484/27/48/48LT02

    Article  Google Scholar 

  24. Guo T, Tang Q, Guo Y et al (2020) Boron quantum dots for photoacoustic imaging-guided photothermal therapy. ACS Appl Mater Inter 13:306–311

    Article  Google Scholar 

  25. Das R, Parveen S, Bora A et al (2020) Origin of high photoluminescence yield and high sers sensitivity of nitrogen-doped graphene quantum dots. Carbon 160:273–286

    Article  CAS  Google Scholar 

  26. Zhen L, Ling C, Qin P et al (2018) Nitrogen and oxygen co-doped graphene quantum dots with high capacitance performance for micro-supercapacitors. Carbon 139:67–75

    Article  Google Scholar 

  27. Sheng ZH, Lin S, Chen JJ et al (2011) Catalyst-free synthesis of nitrogen-doped graphene via thermal annealing graphite oxide with melamine and its excellent electrocatalysis. ACS Nano 5:4350–4385

    Article  CAS  Google Scholar 

  28. Zhang Z, Huang J, Zhang M et al (2015) Ultrathin hexagonal SnS2 nanosheets coupled with g-C3N4 nanosheets as 2D/2D heterojunction photocatalysts toward high photocatalytic activity. Appl Catal B-Environ 163:298–305

    Article  CAS  Google Scholar 

  29. Tao Y, Tang Y, Ling L et al (2017) Size-dependent Ag2S nanodots for second near-infrared fluorescence/photoacoustics imaging and simultaneous photothermal therapy. ACS Nano 11:1848–1857

    Article  Google Scholar 

  30. Zhang QH, Guo QB, Chen Q et al (2020) Highly efficient 2D NIR-II photothermal agent with fenton catalytic activity for cancer synergistic photothermal-chemodynamic therapy. Adv Sci 7:1902576

    Article  CAS  Google Scholar 

  31. Huang L, Li Y, Du Y et al (2019) Mild photothermal therapy potentiates anti-PD-L1 treatment for immunologically cold tumors via an all-in-one and all-in-control strategy. Nat Commun 10:1–15

    Article  Google Scholar 

  32. Hu J, Wang J, Tang W et al (2020) PEGylated polypyrrole-gold nanocomplex as enhanced photothermal agents against tumor cells. J Mater Sci 55:5587–5599. https://doi.org/10.1007/s10853-020-04384-1

    Article  CAS  Google Scholar 

  33. Zhang Z, Shi J, Song Z et al (2017) A synergistically enhanced photothermal transition effect from mesoporous silica nanoparticles with gold nanorods wrapped in reduced graphene oxide. J Mater Sci 53:1810–1823. https://doi.org/10.1007/s10853-017-1628-y

    Article  CAS  Google Scholar 

  34. Xuan Y, Zhang RY, Zhao DH et al (2019) Ultrafast synthesis of gold nanosphere cluster coated by graphene quantum dot for active targeting PA/CT imaging and near-infrared laser/pH-triggered chemo-photothermal synergistic tumor therapy. Chem Eng J 369:87–99

    Article  CAS  Google Scholar 

  35. Zhang L, Xia J, Zhao Q et al (2010) Functional graphene oxide as a nanocarrier for controlled loading and targeted delivery of mixed anticancer drugs. Small 6:537–544

    Article  CAS  Google Scholar 

  36. Liu Z, Sun X, Nakayama-Ratchford N et al (2007) Supramolecular chemistry on water-soluble carbon nanotubes for drug loading and delivery. ACS Nano 1:50–56

    Article  Google Scholar 

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Acknowledgements

This study was financially supported by the National Natural Science Foundation of China (82001894/21606041/21503035/21776044) and Joint Research Fund Liaoning-Shenyang National Laboratory for Materials Science (2019JH3/30100011).

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Author notes

  1. Meng Guan and Yang Xuan contributed equally to this work.

Authors and Affiliations

  1. Key Lab of Biotechnology and Bioresources Utilization of Ministry of Education, College of Life Science, Dalian Minzu University, Dalian, 116600, Liaoning, China

    Meng Guan, Yang Xuan, Yating Gao, Yining Liu & Shubiao Zhang

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  1. Meng Guan
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  2. Yang Xuan
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  3. Yating Gao
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  4. Yining Liu
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Correspondence to Shubiao Zhang.

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Guan, M., Xuan, Y., Gao, Y. et al. Synthesis of Ag@GQD and their application in photoacoustic imaging and chemical/photothermal combination therapy and bacteriostasis. J Mater Sci 57, 7056–7067 (2022). https://doi.org/10.1007/s10853-022-06968-5

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  • DOI: https://doi.org/10.1007/s10853-022-06968-5

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