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Synthesis and characterization of gold-conjugated Backhousia citriodora nanoparticles and their anticancer activity against MCF-7 breast and HepG2 liver cancer cell lines

  • Biomaterials
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Abstract

Environmentally benign-synthesized gold nanoparticles (Au-NPs) have received substantial attention owing to their biomedical applications, particularly in cancer therapy. In the current study, Backhousia citriodora (B. citriodora) leaf extract was applied as a reducing agent for one-pot synthesis of controlled size Au-NPs. The effect of various parameters such as reaction time, pH, and B. citriodora leaf boiling time on the synthesis of Au-NPs was studied. The characterization of the Au-NPs synthesized at 15.0-min incubation time showed colour change because of the surface plasma resonance band around 530.0 nm. TEM photographs showed spherical morphologies with an average size of 8.40 ± 0.084 nm and zeta potential value was − 29.74 mV, indicating stability of the nanoparticles. The biomedical properties of Au-NPs and B. citriodora leaf extract showed strong DPPH radical scavenging. The in vitro anticancer activity determined using MTT assay exhibited that Au-NPs showed a significant dose-dependent reduction in the viability of the MCF-7 breast cancer cell line and the HepG2 liver cancer cell line with IC50 values of 116.65 and 108.21 µg, respectively.

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Acknowledgements

The authors would like to thank the Charles Darwin University for its financial support (faculty research Grant), the Central Analytical Research Facility (CARF) operated by the Institute for Future Environments, Queensland University of Technology (QUT) for SEM, TEM, and XRD facilities and Dr. Sivanesan Arumugam at the Future Industries Institute (FII), University of South Australia, for assistance with obtaining zeta potential values in this research.

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Authors and Affiliations

  1. School of Psychological and Clinical Sciences, Faculty of Engineering, Health, Science and the Environment, Charles Darwin University, Darwin, 0909, Australia

    Roshanak Khandanlou & Vinuthaa Murthy

  2. Department of Biological Sciences, Sunandan Divatia School of Science, NMIMS (Deemed to be) University, Mumbai, Maharashtra, 400056, India

    Dhananjaya Saranath & Hetal Damani

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  1. Roshanak Khandanlou
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  2. Vinuthaa Murthy
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Correspondence to Roshanak Khandanlou.

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Khandanlou, R., Murthy, V., Saranath, D. et al. Synthesis and characterization of gold-conjugated Backhousia citriodora nanoparticles and their anticancer activity against MCF-7 breast and HepG2 liver cancer cell lines. J Mater Sci 53, 3106–3118 (2018). https://doi.org/10.1007/s10853-017-1756-4

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