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Well-oriented bioarchitecture for immobilization of chloroperoxidase on graphene oxide nanosheets by site-specific interactions and its catalytic performance

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Abstract

Chloroperoxidase (CPO) was immobilized on graphene oxide (GO) nanosheets via site-specific interactions of concanavalin (ConA) with saccharide groups on the CPO surface to make GO-ConA-CPO nano-architectonics. Enzymatic oxidative decolorization of malachite green was used to investigate the catalytic performance of GO-ConA-CPO. The GO-ConA-CPO showed high activity (based on the decolorization efficiency), i.e., 93.68% in 15 min. Moreover, GO-ConA-CPO showed better thermostability and remained higher activity against pH change and high concentrations of oxidant H2O2 compared with GO-CPO and the free enzyme. When incubated at 60 °C for 1.5 h, 63.02% of the activity of GO-ConA-CPO and 35.75% of GO-CPO remained compared with that at 25 °C, while free CPO remained only 8.46% in the same condition. The tolerance of GO-ConA-CPO to H2O2 improved from 2.5 to 6 mmol L−1, and the suitable pH range enlarged from 2.5–3.0 to 2.0–4.5. After 8 cycles, the GO-ConA-CPO can keep 52% activity of that in the first run. The enzymatic kinetic constants indicated that introducing CPO into GO-ConA-CPO bioarchitecture improved the diffusion of reactants and products.

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Acknowledgements

This work is supported by the National Natural Science Foundation of China (21176150) the Fundamental Research Funds for the Central Universities (GK201701003).

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, Shaanxi Normal University, No. 620 West Chang’an Road, Chang’an District, Xi’an, 710119, People’s Republic of China

    Yu Ding, Ru Cui, Mancheng Hu, Shuni Li, Quanguo Zhai & Yucheng Jiang

  2. Key Laboratory of Macromolecular Science of Shaanxi Province, Shaanxi Normal University, Xi’an, 710062, People’s Republic of China

    Mancheng Hu, Shuni Li, Quanguo Zhai & Yucheng Jiang

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  1. Yu Ding
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  2. Ru Cui
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  3. Mancheng Hu
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Correspondence to Yucheng Jiang.

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Ding, Y., Cui, R., Hu, M. et al. Well-oriented bioarchitecture for immobilization of chloroperoxidase on graphene oxide nanosheets by site-specific interactions and its catalytic performance. J Mater Sci 52, 10001–10012 (2017). https://doi.org/10.1007/s10853-017-1202-7

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