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Immobilizing enzymes in regular-sized gelatin microspheres through a membrane emulsification method

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Abstract

Enzyme immobilization is a promising biotechnology which not only ensures reusability of enzymes and permits easy biocatalyst-product separation, but also improves the storage stability of enzymes. It overcomes many drawbacks of free enzyme, and hence presents significant applications in bioscience and bioengineering. Microspheres composed of biopolymer hydrogel are widely used as carriers for enzyme immobilization. In this study, the regular-sized gelatin microspheres were prepared using the membrane emulsification method, and were subsequently utilized for the encapsulation of yeast alcohol dehydrogenase (YADH). The results indicated that the recycling stability, pH tolerance, and storage stability of the immobilized enzyme are all significantly improved. The immobilized YADH maintained higher relative activity than its free form under extreme acidic conditions down to pH 4.0 (63 vs. 16 %) and alkaline conditions up to pH 10.0 (42 vs. 0 %). Additionally, microspheres of different sizes can be prepared using membranes of different pore sizes. The further experiment established a linear correlation between the average diameter of microspheres and the pore size of membranes. The effects of preparation conditions on the diameter and uniformity of microspheres were also investigated systematically.

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Acknowledgements

The authors thank the financial support from the National Natural Science Foundation of China (21306139, 21276060), Natural Science Foundation of Tianjin (No. 14JCYBJC20800), the Project (2015LG07) from Tianjin University of Science &Technology and National Undergraduate Training Programs for Innovation and Entrepreneurship (201510057135).

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    Authors and Affiliations

    1. School of Material Science and Chemical Engineering, Tianjin University of Science and Technology, 300457, Tianjin, People’s Republic of China

      Jian Li, Jun Ma, Tao Jiang, Yanhuan Wang, Yanhui Chen & Shuo Liu

    2. School of Chemical Engineering and Technology, Hebei University of Technology, 300100, Tianjin, People’s Republic of China

      Yanjun Jiang

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    1. Jian Li
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    2. Jun Ma
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    Correspondence to Jian Li or Tao Jiang.

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    Jian Li and Jun Ma have contributed equally.

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    Li, J., Ma, J., Jiang, Y. et al. Immobilizing enzymes in regular-sized gelatin microspheres through a membrane emulsification method. J Mater Sci 51, 6357–6369 (2016). https://doi.org/10.1007/s10853-016-9932-5

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