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Synthesis of water-compatible surface-imprinted composite microspheres with core–shell structure for selective recognition of thymopentin from aqueous solution

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Abstract

A novel water-compatible surface-imprinted core–shell microsphere, which had multiple non–covalent interactions with template molecule, was successfully prepared by the surface grafting polymerization method in acetonitrile–water systems with thymopentin as template through ionic liquid-functionalized polyethyleneglycolmethacrylate-co-vinylimidazole microsphere as the matrix. The average diameter of matrix was 1 μm ± 20 nm and the thickness of imprinted layer was about 50 nm. The results of static adsorption experiments indicated that ionic liquid-functionalized molecularly imprinted microspheres showed the good adsorption capacity and specific recognition for template peptide. The binding-isotherm analysis showed that Langmuir isotherm models gave a good fit in the range of concentrations, suggesting that there was only one kind of binding site in imprinted layer. Measurements of the binding kinetics revealed that surface-imprinted composite microspheres reached peptide-adsorption equilibrium in 60 min and the maximum adsorption capacity for TP5 was 38.4 mg g−1. The effects of pH, salt concentration, and temperature on the adsorption capacities were investigated. The microspheres were found to have a high specificity for TP5 with little affinity for BSA and Hb. Finally, the core–shell microspheres can be reused with only 15.6 % decrease in TP5 adsorption capacity after six times.

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Acknowledgements

The authors were grateful to the support of the National Natural Science Foundation of China (Grant Nos. 21174111).

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Authors and Affiliations

  1. Key Laboratory of Applied Physics and Chemistry in Space of Ministry of Education, School of Science, Northwestern Polytechnical University, Xi’an, 710072, China

    Chunbao Du, Xiaoling Hu, Ping Guan, Longxia Guo, Liwei Qian, Ji Li, Renyuan Song & Yimei Tang

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  1. Chunbao Du
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Correspondence to Chunbao Du.

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Du, C., Hu, X., Guan, P. et al. Synthesis of water-compatible surface-imprinted composite microspheres with core–shell structure for selective recognition of thymopentin from aqueous solution. J Mater Sci 50, 427–438 (2015). https://doi.org/10.1007/s10853-014-8602-8

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