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Improvement in physical properties and cytocompatibility of zein by incorporation of pea protein isolate

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Abstract

A series of protein-based biocomposites was prepared from glycerol-plasticized zein (ZE) and pea protein isolate (PPI) using a hot press and moulding process. The effects of PPI content (W PPI) on the structure and properties of ZE/PPI films were investigated. With an increase in W PPI from 0 to 100%, the elongation at break of the films increased from 2.4 to 62.6%, and the water contact angle decreased from 31.8° to 5.8°. Cell toxicity and cytocompatibility of ZE/PPI films were evaluated in vitro. The cell viability of L929 cells cultured in extracts from ZE/PPI films containing 10–30% PPI was higher than that from other films and the control group. The L929 cells expanded very well on the surfaces of films containing 10–30% PPI. Incorporation of 10–30% PPI into ZE improved flexibility, surface hydrophilicity, cytocompatibility and its potential as biomaterials in zein-based composites.

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Acknowledgements

This work was financially supported in part by Canadian Biomass Innovation Network (CBIN) TID 824 and Agricultural Bioproducts Innovation Program of Canada via ABIP-140 to the Pulse Research Network (PURENet). Thanks for the support from Opening Laboratory for Overseas Scientists and Research Center of Medicine, Wuhan University, China.

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Authors and Affiliations

  1. Department of Biomedical Engineering, School of Basic Medical Science, Wuhan University, Wuhan, China

    Chaoming Liu, Yun Chen & Xiaomei Wang

  2. Department of Stomatology, General Hospital of Jianghan Oil Field, Qianjiang, China

    Chaoming Liu

  3. College of Chemical Engineering, Wuhan University of Technology, Wuhan, China

    Jin Huang

  4. Bioproducts and Bioprocesses National Science Program, Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, S7N 0X2, SK, Canada

    Yun Chen, Peter R. Chang & Debbie P. Anderson

Authors
  1. Chaoming Liu
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  2. Yun Chen
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  3. Xiaomei Wang
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  4. Jin Huang
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  5. Peter R. Chang
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  6. Debbie P. Anderson
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Correspondence to Yun Chen or Peter R. Chang.

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Liu, C., Chen, Y., Wang, X. et al. Improvement in physical properties and cytocompatibility of zein by incorporation of pea protein isolate. J Mater Sci 45, 6775–6785 (2010). https://doi.org/10.1007/s10853-010-4774-z

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  • DOI: https://doi.org/10.1007/s10853-010-4774-z

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