Abstract
The application of mesoporous silica and silica–titania-mixed metal oxide films prepared via sol–gel processing as substrates for cell growth was investigated. A deliberate tailoring of the chemical composition of the porous substrates with different Si:Ti ratios was achieved by using a single-source precursor based on a titanium-coordinated alkoxysilane, resulting in mesoporous silica–titania films with hydrophilic surfaces. The different coatings were investigated with respect to their applicability in the cultivation of human cells such as human fibroblasts and multipotent mesenchymal stromal cells. It was found that they promoted cell adhesion and proliferation of human fibroblasts up to a period of 14 days. After 2 weeks only single apoptotic cells could be detected on silica–titania mixed oxide films in contrast to a somewhat higher amount on silica coatings. Furthermore, none of the films inhibited osteogenic differentiation of multipotent mesenchymal stromal cells.
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Kresge CT, Leonowicz ME et al (1992) Nature 359:710
Carturan G, Campostrini et al (1989) J Mol Catal 57:L13
Avnir D, Coradin T, Lev O, Livage J (2006) J Mater Chem 16:1013
Rooke JC, Léonard A et al (2008) J Mater Chem 18:2833
Yang XY, Li ZQ et al (2006) Adv Mater 18:410
Vallet-Regi M (2006) Chem Europ J 12:5934
Coffer JL, Whitehead MA et al (2005) Phys Stat Sol A Appl Mater Sci 202:1451
Yan X, Yu C et al (2004) Angew Chem Int Ed 43:5980
Balas F, Manzano M et al (2008) Acta Biomater 4:514
Baca HK, Carnes E et al (2006) Science 313:337
Puleo DA, Nanci A (1999) Biomaterials 20:2311
Vallet-Regi M (2006) Dalton Trans 44:5211
Viitala R, Jokinen M et al (2002) Biomaterials 23:3073
Ääritalo V, Areva S et al (2007) J Mater Sci Mater Med 18:1863
Areva S, Ääritalo V et al (2007) J Mater Sci Mater Med 18:1633
Brinker CJ, Lu Y et al (1999) Adv Mater 11:579
Doshi D, Hüsing N et al (2000) Science 290:107
Hozumi A, Kimura T (2008) Langmuir 24:11141
Spatz JP (2004) Nanobiotechnology 53
Puchberger M, Rupp W et al (2004) New J Chem 28:1289
Torma V, Peterlik H et al (2005) Chem Mater 17:3146
Brinker CJ, Raman NK et al (1995) J Sol-Gel Sci Technol 4:117
Fiedler J, Brill C et al (2006) Biochem Biophys Res Commun 345:1177
Lu Y, Ganguli R et al (1997) Nature 389:364
Garcia C, Zhang Y et al (2003) Angew Chem Int Ed 42:1526
Matsura V, Guari Y et al (2004) J Mater Chem 14:3026
Tura C, Coombs N et al (2005) Chem Mater 17:573
Supplit R, Hüsing N et al (2006) J Mater Chem 16:4443
Hüsing N, Launay B (2003) Appl Catal 254:297
Supplit R, Hüsing N et al (2007) Europ J Inorg Chem 18:2797
Koehler J, Cai J et al (2007) Mater Res Soc Symp Proc 1007:S04–S12
Andrianainarivelo M, Corriu R et al (1996) J Mater Chem 6:1665
Kanta A, Sedev R et al (2005) Langmuir 21:2400
Lebaron RG, Athanasiou KA (2000) Tissue Eng 6:85
Hench LL (2000) Key Eng Mater 192–195:575
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Böcking, D., Fiedler, J., Brenner, R.E. et al. Cultivation of human fibroblasts and multipotent mesenchymal stromal cells on mesoporous silica and mixed metal oxide films. J Mater Sci 44, 6786–6794 (2009). https://doi.org/10.1007/s10853-009-3565-x
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DOI: https://doi.org/10.1007/s10853-009-3565-x