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Supramolecular encapsulation of doxorubicin with β-cyclodextrin dendrimer: in vitro evaluation of controlled release and cytotoxicity

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Abstract

In this study, the formation of supramolecular inclusion complex of doxorubicin (DOX), a high loading and pH-dependent delivery of DOX on β-CD dendrimer was studied. β-cyclodextrin (β-CD) dendrimer having β-CD in both periphery and core was prepared with entrapment efficiency using click reaction. The encapsulation property of the β-CD-dendrimer was investigated by DOX as model drug. The chemical construction of β-CD-dendrimer was described by 1H NMR, 13C NMR and FTIR and its inclusion complex construction was studied by FTIR, DSC, SEM, and DLS techniques. It was confirmed that β-CD dendrimer able to encapsulate DOX in solution; as a result, the designed complex revealed pH-dependent sustained release of DOX, in vitro. Also, the in vitro outcomes on T47D cells displayed that complexation of DOX with β-CD dendrimer involved an improvement of in vitro cytotoxicity and anticancer activity and this data appeared to be as a result of the developed solubility of the DOX.

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Acknowledgements

Authors gratefully acknowledge the financial support from the University of Tabriz and RCPN of Tabriz University of Medical Science.

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Authors and Affiliations

  1. Laboratory of Dendrimers and Nano-Biopolymers, Faculty of Chemistry, University of Tabriz, Tabriz, Iran

    Toomari Yousef & Namazi Hassan

  2. Research Center for Pharmaceutical Nanotechnology (RCPN), Tabriz University of Medical Science, Tabriz, Iran

    Toomari Yousef & Namazi Hassan

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  1. Toomari Yousef
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Correspondence to Namazi Hassan.

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Yousef, T., Hassan, N. Supramolecular encapsulation of doxorubicin with β-cyclodextrin dendrimer: in vitro evaluation of controlled release and cytotoxicity. J Incl Phenom Macrocycl Chem 87, 105–115 (2017). https://doi.org/10.1007/s10847-016-0682-4

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