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DNA cleavage promoted by Cu2+ complex of cyclen containing pyridine subunit

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Abstract

The tetraazamacrocycle crown ether (cyclen) containing two pyridine subunits was prepared by a modified procedure and the interaction of its metal complexes with DNA was studied by agarose gel electrophoresis analysis. The results indicate that the Cu2+ complex as nuclease model can promote the hydrolysis of phosphodiester bond of supercoiled DNA. The rate of degradation of the supercoiled DNA (form I) to nicked DNA (form II) obtained at physiological condition in the presence of 2.14 mM Cu2+ complex is 2.31 × 10–3 min−1. The dependence of the rate of supercoiled DNA cleavage from the complex concentration shows an unusual profile and a hydrolytic cleaving mechanism of two monometallic complexes through cooperation from two-point binding to DNA is proposed.

Graphical abstract

DNA cleavage promoted by metal complex of cyclen containing pyridine subunit

Ying Li, Xiao-Min Lu, Xin Sheng, Guo-Yuan Lu*, Ying Shao and Qiang Xu*

The copper complex of tetraazamacrocycle crown ether (cyclen) containing two pyridine subunits can promote the hydrolysis of phosphodiester bond of supercoiled DNA and a hydrolytic mechanism of two monometallic complexes through cooperation from two-point binding to DNA is proposed.

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Acknowledgements

Financial supports from the National Science Foundation (Grant. No. 20372032) of China and the Analytical Foundation of Nanjing University are deeply acknowledged.

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Authors and Affiliations

  1. Department of Chemistry, State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing, 210093, P.R. China

    Ying Li, Xin Sheng, Guo-Yuan Lu & Ying Shao

  2. State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210093, P.R. China

    Xiao-Min Lu & Qiang Xu

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  1. Ying Li
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  2. Xiao-Min Lu
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  4. Guo-Yuan Lu
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Li, Y., Lu, XM., Sheng, X. et al. DNA cleavage promoted by Cu2+ complex of cyclen containing pyridine subunit. J Incl Phenom Macrocycl Chem 59, 91–98 (2007). https://doi.org/10.1007/s10847-007-9299-y

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