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The evaluation effect of nanoliposome-loaded Mito-Tempo on sperm parameters during human sperm cryopreservation

  • Gamete Biology
  • Published:
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Abstract

Aim

The aim of this study is the evaluation effect of nanoliposome-loaded Mito-Tempo on sperm parameters during human sperm cryopreservation.

Methods

Semen samples of 50 Asthenoteratozoospermia men (random) were collected. Sperm parameters were analyzed based on World Health Organization (WHO, 2010) criteria (2021) and each sample was divided into 5 groups (E1–E5). E1 (control group): the sperm was cryopreserved without nanoliposome, and Mito-Tempo. E2: sperm cryopreservation with Mito-Tempo-loaded nanoliposome (Mito-Tempo 0.1 mM) + freezing medium. E3: sperm cryopreservation with Mito-Tempo-loaded nanoliposome (Mito-Tempo 0.2 mM) + freezing medium. E4: in this group, the cryopreservation sperm with Mito-Tempo 0.3 mM + freezing medium. E5: the cryopreservation sperm with Mito-Tempo 0.2 mM + freezing medium.

Results

The result of this study indicated that sperm parameters and total antioxidant capacity (TAC) significantly increase in E3 and E4 groups, compared to E1, E2, and E5 groups respectively (P < 0.05). The percentage of abnormal morphology, DNA fragmentation index (DFI), malondialdehyde (MDA), and the levels of ROS significantly decrease in E3 and E4 groups, compared to E1, E2, and E5 groups (P < 0.05). In addition, the sperm parameters and stress oxidative factors significantly improve in E3 group compared to other groups (P < 0.05).

Conclusions

In conclusion, the combination of Mito-Tempo with nanoliposome due to its ability to cooperate with lipid layers may lead to significant performance in reducing oxidative stress damage and increasing the quality of sperm parameters.

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Data availability

The primary data for this study is available from the authors upon direct request.

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Acknowledgements

This study was supported by IVF Unit of Infertility, and Research Center of the ACECR, Qom.

Author information

Authors and Affiliations

  1. Department of Reproductive Biology, Academic Center for Education, Culture, and Research (ACECR), Qom, Iran

    Rahil Jannatifar, Seyedeh Saeideh Sahraei, Atefeh Verdi & Elham Asa

  2. Department of Biology, Faculty of Science, Arak University, Arak, Iran

    Hamid Piroozmanesh

  3. Infertility Treatment Center of ACECR, Bonyad Street, P.O. box: 3713746611, Qom, Iran

    Rahil Jannatifar

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  1. Rahil Jannatifar
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Correspondence to Rahil Jannatifar.

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Jannatifar, R., Piroozmanesh, H., Sahraei, S.S. et al. The evaluation effect of nanoliposome-loaded Mito-Tempo on sperm parameters during human sperm cryopreservation. J Assist Reprod Genet (2024). https://doi.org/10.1007/s10815-024-03132-7

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