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Preimplantation genetic testing for aneuploidies does not increase success rates in fresh oocyte donation cycles: a paired cohort study

  • Assisted Reproduction Technologies
  • Published:
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Abstract

Purpose

To determine whether in vitro fertilization cycles using fresh oocyte donations benefit from preimplantation genetic testing for aneuploidies.

Methods

A paired cohort study compared 44 fresh oocyte donation cycles with or without preimplantation genetic testing for aneuploidy (PGT-A). The sibling oocyte study analyzed fertilized oocytes, blastocyst development, and euploidy rate. Only frozen embryo transfers were performed. Pregnancy, implantation, biochemical pregnancy, miscarriage, stillbirth, live birth, and twin pregnancy rates were analyzed between groups.

Results

Fresh oocyte donation cycles between PGT-A and non-PGT-A groups were similar in all laboratory and clinical outcomes. A euploidy rate of 74.2% was observed in the PGT-A group. Although a slight trend was observed for implantation rate in the PGT-A group, it was not statistically significant. No difference was observed for live birth between groups.

Conclusion

PGT-A associated with fresh oocyte donation cycles does not improve clinical outcomes and can be seen as over-treatment for patients.

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References

  1. Ubaldi FM, Cimadomo D, Vaiarelli A, Fabozzi G, Venturella R, Maggiulli R, et al. Advanced maternal age in IVF: still a challenge? The present and the future of its treatment. Front Endocrinol (Lausanne) [Internet]. 2019. 10. Available from: https://www.frontiersin.org/article/, https://doi.org/10.3389/fendo.2019.00094/full

  2. Franasiak JM, Forman EJ, Hong KH, Werner MD, Upham KM, Treff NR, et al. The nature of aneuploidy with increasing age of the female partner: a review of 15,169 consecutive trophectoderm biopsies evaluated with comprehensive chromosomal screening. Fertil Steril [Internet]. 2014. 101:656–663.e1. Available from: https://linkinghub.elsevier.com/retrieve/pii/S0015028213032573

  3. Viotti M. Preimplantation genetic testing for chromosomal abnormalities: aneuploidy, mosaicism, and structural rearrangements. Genes (Basel) [Internet]. 2020. 11:602. Available from: https://www.mdpi.com/2073-4425/11/6/602

  4. Crawford NM, Steiner AZ. Age-related Infertility. Obstet Gynecol Clin North Am. 2015;42:15–25.

    Article  Google Scholar 

  5. Daar J, Benward J, Collins L, Davis J, Francis L, Gates E, et al. Oocyte or embryo donation to women of advanced reproductive age: an Ethics Committee opinion. Fertil Steril [Internet]. 2016. 106:e3–7. Available from: https://linkinghub.elsevier.com/retrieve/pii/S001502821661405X

  6. Kushnir VA, Gleicher N. Fresh versus cryopreserved oocyte donation. Curr Opin Endocrinol Diabetes Obes. 2016;23:451–7.

    Article  CAS  Google Scholar 

  7. Barad DH, Darmon SK, Kushnir VA, Albertini DF, Gleicher N. Impact of preimplantation genetic screening on donor oocyte-recipient cycles in the United States. Am J Obstet Gynecol [Internet]. 2017. 217:576.e1–576.e8. Available from: https://linkinghub.elsevier.com/retrieve/pii/S0002937817308657

  8. Doyle N, Gainty M, Eubanks A, Doyle J, Hayes H, Tucker M, et al. Donor oocyte recipients do not benefit from preimplantation genetic testing for aneuploidy to improve pregnancy outcomes. Hum Reprod [Internet]. 2020. 35:2548–55. Available from: https://academic.oup.com/humrep/article/35/11/2548/5923786

  9. Masbou AK, Friedenthal JB, McCulloh DH, McCaffrey C, Fino ME, Grifo JA, et al. A comparison of pregnancy outcomes in patients undergoing donor egg single embryo transfers with and without preimplantation genetic testing. Reprod Sci [Internet]. 2019. 26:1661–5. Available from: http://journals.sagepub.com/doi/, https://doi.org/10.1177/1933719118820474

  10. Peyser A, Brownridge S, Rausch M, Noyes N. The evolving landscape of donor egg treatment: success, women’s choice, and anonymity. J Assist Reprod Genet [Internet]. 2021. Available from: https://link.springer.com/, https://doi.org/10.1007/s10815-021-02262-6

  11. Almodin CG, Minguetti-Camara VC, Paixao CL, Pereira PC. Embryo development and gestation using fresh and vitrified oocytes. Hum Reprod [Internet]. 2010. 25:1192–8. Available from: https://academic.oup.com/humrep/article-lookup/doi/, https://doi.org/10.1093/humrep/deq042

  12. Gardner DK, Lane M, Stevens J, Schlenker T, Schoolcraft WB. Blastocyst score affects implantation and pregnancy outcome: towards a single blastocyst transfer. Fertil Steril [Internet]. 2000. 73:1155–8. Available from: https://linkinghub.elsevier.com/retrieve/pii/S0015028200005185

  13. Dahdouh EM, Balayla J, García-Velasco JA. Comprehensive chromosome screening improves embryo selection: a meta-analysis. Fertil Steril [Internet]. 2015. 104:1503–12. Available from: https://linkinghub.elsevier.com/retrieve/pii/S0015028215018841

  14. Rubio C, Rodrigo L, Garcia-Pascual C, Peinado V, Campos-Galindo I, Garcia-Herrero S, et al. Clinical application of embryo aneuploidy testing by next-generation sequencing. Gardner DK, editor. Biol Reprod [Internet]. 2019. 101:1083–90. Available from: https://academic.oup.com/biolreprod/article/101/6/1083/5306439

  15. Sills ES, Li X, Frederick JL, Khoury CD, Potter DA. Determining parental origin of embryo aneuploidy: analysis of genetic error observed in 305 embryos derived from anonymous donor oocyte IVF cycles. Mol Cytogenet [Internet]. 2014. 7:68. Available from: http://molecularcytogenetics.biomedcentral.com/articles/, https://doi.org/10.1186/s13039-014-0068-5

  16. Hoyos LR, Cheng CY, Brennan K, Hubert G, Wang B, Buyalos RP, et al. Euploid rates among oocyte donors: is there an optimal age for donation? J Assist Reprod Genet [Internet]. 2020. 37:589–94. Available from: http://link.springer.com/, https://doi.org/10.1007/s10815-020-01694-w

  17. Ozgur K, Berkkanoglu M, Bulut H, Yoruk GDA, Candurmaz NN, Coetzee K. Single best euploid versus single best unknown-ploidy blastocyst frozen embryo transfers: a randomized controlled trial. J Assist Reprod Genet [Internet]. 2019. 36:629–36. Available from: http://link.springer.com/, https://doi.org/10.1007/s10815-018-01399-1

  18. Haddad G, Deng M, Wang CT, Witz C, Williams D, Griffith J, et al. Assessment of aneuploidy formation in human blastocysts resulting from donated eggs and the necessity of the embryos for aneuploidy screening. J Assist Reprod Genet [Internet]. 2015. 32:999–1006. Available from: http://link.springer.com/, https://doi.org/10.1007/s10815-015-0492-4

  19. García-Ferreyra J, Luna D, Villegas L, Romero R, Zavala P, Hilario R, et al. High aneuploidy rates observed in embryos derived from donated oocytes are related to male aging and high percentages of sperm DNA fragmentation. Clin Med Insights Reprod Heal [Internet]. 2015. 9:CMRH.S32769. Available from: http://journals.sagepub.com/doi/, https://doi.org/10.4137/CMRH.S32769

  20. García-Ferreyra J, Hilario R, Dueñas J. High percentages of embryos with 21, 18 or 13 trisomy are related to advanced paternal age in donor egg cycles. JBRA Assist Reprod [Internet]. 2018. Available from: http://www.gnresearch.org/doi/, https://doi.org/10.5935/1518-0557.20180004

  21. Carrasquillo RJ, Kohn TP, Cinnioglu C, Rubio C, Simon C, Ramasamy R, et al. Advanced paternal age does not affect embryo aneuploidy following blastocyst biopsy in egg donor cycles. J Assist Reprod Genet [Internet]. 2019. 36:2039–45. Available from: http://link.springer.com/, https://doi.org/10.1007/s10815-019-01549-z

  22. Munné S, Alikani M, Ribustello L, Colls P, Martínez-Ortiz PA, McCulloh DH. Euploidy rates in donor egg cycles significantly differ between fertility centers. Hum Reprod [Internet]. 2017. 32:743–9. Available from: https://academic.oup.com/humrep/article/32/4/743/3059568

  23. Coates A, Bankowski BJ, Kung A, Griffin DK, Munne S. Differences in pregnancy outcomes in donor egg frozen embryo transfer (FET) cycles following preimplantation genetic screening (PGS): a single center retrospective study. J Assist Reprod Genet [Internet]. 2017. 34:71–8. Available from: http://link.springer.com/https://doi.org/10.1007/s10815-016-0832-z

  24. Scott RT, Upham KM, Forman EJ, Zhao T, Treff NR. Cleavage-stage biopsy significantly impairs human embryonic implantation potential while blastocyst biopsy does not: a randomized and paired clinical trial. FertilSteril [Internet]. 2013. 100:624–30. Available from: https://linkinghub.elsevier.com/retrieve/pii/S0015028213005530

  25. Racca A, Drakopoulos P, Van Landuyt L, Willem C, Santos-Ribeiro S, Tournaye H, et al. Single and double embryo transfer provide similar live birth rates in frozen cycles. Gynecol Endocrinol [Internet]. 2020. 36:824–8. Available from: https://www.tandfonline.com/doi/full/, https://doi.org/10.1080/09513590.2020.1712697

  26. Neal SA, Morin SJ, Franasiak JM, Goodman LR, Juneau CR, Forman EJ, et al. Preimplantation genetic testing for aneuploidy is cost-effective, shortens treatment time, and reduces the risk of failed embryo transfer and clinical miscarriage. Fertil Steril [Internet]. 2018. 110:896–904. Available from: https://linkinghub.elsevier.com/retrieve/pii/S0015028218304977

  27. Facadio Antero M, Singh B, Pradhan A, Gornet M, Kearns WG, Baker V, et al. Cost-effectiveness of preimplantation genetic testing for aneuploidy for fresh donor oocyte cycles. F&S Reports [Internet]. 2021. 2:36–42. Available from: https://linkinghub.elsevier.com/retrieve/pii/S2666334120301240

  28. Scriven PN. Towards a better understanding of preimplantation genetic screening for aneuploidy: insights from a virtual trial for women under the age of 40 when transferring embryos one at a time. Reprod Biol Endocrinol [Internet]. 2017. 15:49. Available from: http://rbej.biomedcentral.com/articles/, https://doi.org/10.1186/s12958-017-0269-y

  29. Lai H-H, Chuang T-H, Wong L-K, Lee M-J, Hsieh C-L, Wang H-L, et al. Identification of mosaic and segmental aneuploidies by next-generation sequencing in preimplantation genetic screening can improve clinical outcomes compared to array-comparative genomic hybridization. Mol Cytogenet [Internet]. 2017. 10:14. Available from: https://molecularcytogenetics.biomedcentral.com/articles/, https://doi.org/10.1186/s13039-017-0315-7

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Authors and Affiliations

  1. Nilo Frantz Reproductive Medicine, Porto Alegre, RS, Brazil

    Carolina Lumertz Martello, Marcos Iuri Roos Kulmann, Luiza Mezzomo Donatti & Nilo Frantz

  2. Basic Health Sciences Institute, Federal University of Rio Grande Do Sul, Porto Alegre, RS, Brazil

    Adriana Bos-Mikich

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  1. Carolina Lumertz Martello
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  2. Marcos Iuri Roos Kulmann
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  3. Luiza Mezzomo Donatti
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Correspondence to Carolina Lumertz Martello.

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All patients had previously given informed consent for the use of their data.

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Martello, C.L., Kulmann, M.I.R., Donatti, L.M. et al. Preimplantation genetic testing for aneuploidies does not increase success rates in fresh oocyte donation cycles: a paired cohort study. J Assist Reprod Genet 38, 2909–2914 (2021). https://doi.org/10.1007/s10815-021-02339-2

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