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Effect of acteoside on the re-localization and abnormal morphology of mitochondria in porcine oocytes during in vitro maturation

  • Gamete Biology
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Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

The aim of this study is to investigate the effect of acteoside, an antioxidant, on in vitro maturation (IVM) of oocytes to improve early parthenogenetic embryonic developmental competence.

Methods

Porcine immature oocytes (total 770) were cultured in IVM medium with acteoside at various concentrations, 0 (control), 10, 30, and 50 μM. Each group was assessed for maturation and subsequent development rates, reactive oxygen species (ROS) level (15 oocytes per group and four independent experiments performed), ultrastructure observation (15 oocytes per group), mitochondrial activity (30 oocytes per groups and three independent experiments performed), and expression patterns of apoptosis-related genes (100 expended parthenogenetic embryos per group and three independent experiment performed). Main outcome measures were the rates of IVM, blastocyst formation, ROS, mitochondria, and expression of apoptosis-related genes in oocytes treated with acteoside.

Result(s)

Addition of acteoside during IVM did not change the maturation efficiency of oocytes but improved the rate of blastocyst formation with significantly decreased ROS level. Moreover, in acteoside-treated oocytes, cytoplasmic maturation was improved with morphologically uniform distribution of mitochondria and lipid droplets in cytoplasm. Acteoside supplementation also increased the mRNA expression levels of antiapoptotic genes and reduced those of pro-apoptotic genes.

Conclusion(s)

Acteoside supplementation in IVM medium improves the oocyte quality and subsequent development of pre-implantation embryos that would eventually contribute to produce embryos with high embryonic development competence.

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Acknowledgments

This study was supported by a grant from the Next-Generation BioGreen 21 program (Grant No. PJ011359) funded by the Rural Development Administration, Republic of Korea. We are thankful to Dr. John Hammond in USDA-ARS for his scientific comments and writing support on the manuscript.

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    Authors and Affiliations

    1. Department of Animal Science and Biotechnology, College of Agriculture and Life Science, Chungnam National University, Daejeon, 305-764, Republic of Korea

      Keun Jung Kim, Ju Lan Chun, Ji Hye Lee, Kang-Sun Park, Kil Woo Han, Bo Myeong Lee, Eun Young Kim & Min Kyu Kim

    2. Department of Biology Education, College of Education, Chonnam National University, Gwangju, 500-757, Republic of Korea

      Kyung-Bon Lee

    3. Department of Pathology, School of Medicine, Chungnam National University, Daejeon, 301-747, Republic of Korea

      Jin Man Kim

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    1. Keun Jung Kim
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    Correspondence to Min Kyu Kim.

    Additional information

    Capsule Acteoside supplementation in IVM medium improves the oocyte quality and subsequent development of pre-implantation embryos that would eventually contribute to produce embryos with high embryonic development competence.

    Keun Jung Kim and Ju Lan Chun contributed equally to this work.

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    Supplement Figure 1

    Active mitochondrial distribution in porcine oocytes matured in vitro with a 0 μM (control) or b 10 μM acteoside. Oocytes were stained with MitoTracker Red FM. Bar = 20 μm (30 oocytes per groups were used, and three independent experiments were performed) (GIF 115 kb)

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    Kim, K.J., Chun, J.L., Lee, KB. et al. Effect of acteoside on the re-localization and abnormal morphology of mitochondria in porcine oocytes during in vitro maturation. J Assist Reprod Genet 33, 939–948 (2016). https://doi.org/10.1007/s10815-016-0729-x

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