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Supplementation with low concentrations of melatonin improves nuclear maturation of human oocytes in vitro

  • Gamete Biology
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

An Erratum to this article was published on 15 March 2014

Abstract

Purpose

Studies in bovine and porcine have indicated that melatonin (MT) could induce meiotic maturation of immature oocytes in vitro. The object of the current study was to investigate if MT could ameliorate human oocytes maturation during rescue in vitro maturation (IVM).

Methods

Two hundred seventy eight germinal vesicle (GV) oocytes and 451 (MI) metaphase I oocytes were vitrified, thawed and then matured in vitro. All the oocytes were randomly allocated into six groups in which the oocytes were cultured in medium supplemented with different concentrations of MT (0, 10−2, 1, 102, 104, 106 nM) and nuclear maturation was evaluated at 6 h, 12 h, 18 h, 24 h and 48 h of culture.

Results

The optimal MT concentration for both GV and MI oocytes was 1 nM. At 24 h of culture, nuclear maturation rate of MI oocytes cultured in 1 nM MT medium was significantly higher than other groups (P < 0.05); Nuclear maturation rate of GV oocytes cultured in 1 nM MT medium was also significantly higher than the control group (P < 0.05). On the other hand, decreased nuclear maturation rate was observed in the high MT concentration group (106 nM).

Conclusions

The current study demonstrated that low concentration of exogenous MT could ameliorate nuclear maturation of human oocyte during rescue IVM, while high concentration of MT presented negative effects.

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References

  1. Balakier H, Sojecki A, Motamedi G, Librach C. Time-dependent capability of human oocytes for activation and pronuclear formation during metaphase II arrest. Hum Reprod. 2004;19(4):982–7.

    Article  PubMed  Google Scholar 

  2. Chan W, Ng T. Development of pre-implantation, mouse embryos under the influence of pineal indoles. J Neural Transm Gen Sect JNT. 1994;96(1):19–29.

    Article  CAS  Google Scholar 

  3. Chian R-C, Lim J-H, Tan S-L. State of the art in in-vitro oocyte maturation. Curr Opin Obstet Gynecol. 2004;16(3):211.

    Article  PubMed  Google Scholar 

  4. Combelles CM, Gupta S, Agarwal A. Could oxidative stress influence the in-vitro maturation of oocytes? Reprod BioMed Online. 2009;18(6):864–80.

    Article  PubMed Central  PubMed  Google Scholar 

  5. Edwards RG. Maturation in vitro of human ovarian oöcytes. Lancet. 1965;2(7419):926.

    Article  CAS  PubMed  Google Scholar 

  6. Gardner DK. Textbook of assisted reproductive techniques: laboratory and clinical perspectives. Taylor & Francis; 2001.

  7. Guerin P, El Mouatassim S, Menezo Y. Oxidative stress and protection against reactive oxygen species in the pre-implantation embryo and its surroundings. Hum Reprod Updat. 2001;7(2):175–89.

    Article  CAS  Google Scholar 

  8. Hardeland R. Atioxidative protection by melatonin. Endocrine. 2005;27(2):119–30.

    Article  CAS  PubMed  Google Scholar 

  9. Harvey AJ, Kind KL, Thompson JG. REDOX regulation of early embryo development. Reproduction. 2002;123(4):479–86.

    Article  CAS  PubMed  Google Scholar 

  10. Ishizuka B, Kuribayashi Y, Murai K, Amemiya A, Itoh MT. The effect of melatonin on in vitro fertilization and embryo development in mice. J Pineal Res. 2000;28(1):48–51.

    Article  CAS  PubMed  Google Scholar 

  11. Jahnke G, Marr M, Myers C, Wilson R, Travlos G, Price C. Maternal and developmental toxicity evaluation of melatonin administered orally to pregnant Sprague–Dawley rats. Toxicol Sci. 1999;50(2):271–9.

    Article  CAS  PubMed  Google Scholar 

  12. Legge M, Sellens MH. Free radical scavengers ameliorate the 2-cell block in mouse embryo culture. Hum Reprod. 1991;6(6):867–71.

    CAS  PubMed  Google Scholar 

  13. Leon J, Acuña-Castroviejo D, Escames G, Tan DX, Reiter RJ. Melatonin mitigates mitochondrial malfunction. J Pineal Res. 2005;38(1):1–9.

    Article  CAS  PubMed  Google Scholar 

  14. McElhinny AS, Davis FC, Warner CM. The effect of melatonin on cleavage rate of C57BL/6 and CB A/Ca preimplantation embryos cultured in vitro. J Pineal Res. 1996;21(1):44–8.

    Article  CAS  PubMed  Google Scholar 

  15. Nogueira D, Sadeu JC, Montagut J. In vitro oocyte maturation: current status. In: Seminars in reproductive medicine. Thieme Medical Publishers; 2012. p. 199–213.

  16. Pang S, Li L, Ayre E, Pang C, Lee P, Xu R, et al. Neuroendocrinology of melatonin in reproduction: recent developments. J Chem Neuroanat. 1998;14(3):157–66.

    Article  CAS  PubMed  Google Scholar 

  17. Papis K, Poleszczuk O, Wenta-Muchalska E, Modlinski JA. Melatonin effect on bovine embryo development in vitro in relation to oxygen concentration. J Pineal Res. 2007;43(4):321–6.

    Article  CAS  PubMed  Google Scholar 

  18. Rao GD, Chian R, Son W, Gilbert L, Tan S. Fertility preservation in women undergoing cancer treatment. Lancet. 2004;363(9423):1829–9.

    Article  PubMed  Google Scholar 

  19. Reiter RJ. Melatonin and human reproduction. Ann Med. 1998;30(1):103–8.

    Article  CAS  PubMed  Google Scholar 

  20. Reiter RJ. Melatonin: clinical relevance. Best Pract Res Clin Endocrinol Metab. 2003;17(2):273–85.

    Article  CAS  PubMed  Google Scholar 

  21. Reiter RJ, Tan D-X, Osuna C, Gitto E. Actions of melatonin in the reduction of oxidative stress. J Biomed Sci. 2000;7(6):444–58.

    Article  CAS  PubMed  Google Scholar 

  22. Shu Y, Gebhardt J, Watt J, Lyon J, Dasig D, Behr B. Fertilization, embryo development, and clinical outcome of immature oocytes from stimulated intracytoplasmic sperm injection cycles. Fertil Steril. 2007;87(5):1022–7.

    Article  PubMed  Google Scholar 

  23. Son W-Y, Chung J-T, Demirtas E, Holzer H, Sylvestre C, Buckett W, et al. Comparison of in-vitro maturation cycles with and without in-vivo matured oocytes retrieved. Reprod BioMed Online. 2008;17(1):59–67.

    Article  PubMed  Google Scholar 

  24. Son W-Y, Lee S-Y, Lim J-H. Fertilization, cleavage and blastocyst development according to the maturation timing of oocytes in in vitro maturation cycles. Hum Reprod. 2005;20(11):3204–7.

    Article  CAS  PubMed  Google Scholar 

  25. Takami M, Preston S, Toyloy V, Behrman HR. Antioxidants reversibly inhibit the spontaneous resumption of meiosis. Am J Physiol Endocrinol Metab. 1999;276(4):E684–8.

    CAS  Google Scholar 

  26. Tamura H, Takasaki A, Taketani T, Tanabe M, Kizuka F, Lee L, et al. The role of melatonin as an antioxidant in the follicle. J Ovarian Res. 2012;5(5):5.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  27. Tan D-X, Manchester LC, Reiter RJ, Plummer BF, Limson J, Weintraub ST, et al. Melatonin directly scavenges hydrogen peroxide: a potentially new metabolic pathway of melatonin biotransformation. Free Radic Biol Med. 2000;29(11):1177–85.

    Article  CAS  PubMed  Google Scholar 

  28. Tan D, Reiter RJ, Manchester LC, Yan M, El-Sawi M, Sainz RM, et al. Chemical and physical properties and potential mechanisms: melatonin as a broad spectrum antioxidant and free radical scavenger. Curr Top Med Chem. 2002;2(2):181–97.

    Article  CAS  PubMed  Google Scholar 

  29. Tomás-Zapico C, Coto-Montes A. A proposed mechanism to explain the stimulatory effect of melatonin on antioxidative enzymes. J Pineal Res. 2005;39(2):99–104.

    Article  PubMed  Google Scholar 

  30. Veeck L, Wortham Jr J, Witmyer J, Sandow B, Acosta A, Garcia J, et al. Maturation and fertilization of morphologically immature human oocytes in a program of in vitro fertilization. Fertil Steril. 1983;39(5):594.

    CAS  PubMed  Google Scholar 

  31. Wei D, Zhang C, Yin B, Wang P, Xie J, Song X, et al. Early cumulus cell removal could reduce the available embryo rate in human IVF. J Assist Reprod Genet. 2011;28(12):1213–6.

    Article  PubMed Central  PubMed  Google Scholar 

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Authors and Affiliations

  1. Reproductive Medicine Center, the People’s Hospital of Zhengzhou University; Henan Provincial People’s Hospital, Zhengzhou, Henan, China

    Duo Wei, Culian Zhang, Juanke Xie, Xiaobing Song, Baoli Yin, Qi Liu, Lin Hu, Haoying Hao & Jiaxuan Geng

  2. Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, Jinan, China

    Peng Wang

Authors
  1. Duo Wei
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  2. Culian Zhang
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  3. Juanke Xie
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  4. Xiaobing Song
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  5. Baoli Yin
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  6. Qi Liu
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  7. Lin Hu
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  8. Haoying Hao
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  9. Jiaxuan Geng
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  10. Peng Wang
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Corresponding author

Correspondence to Culian Zhang.

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Funding information

The current study is funded by Major Program of Key Medical Science Project of Henan Province (Funding Number:201001010).

Capsule Low concentration of exogenous melatonin could ameliorate nuclear maturation of human oocyte during rescue IVM, while high concentration of melatonin presented negative effects.

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Wei, D., Zhang, C., Xie, J. et al. Supplementation with low concentrations of melatonin improves nuclear maturation of human oocytes in vitro. J Assist Reprod Genet 30, 933–938 (2013). https://doi.org/10.1007/s10815-013-0021-2

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  • DOI: https://doi.org/10.1007/s10815-013-0021-2

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