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Oxidative markers in cryopreservation medium from frozen-thawed embryos: a possible tool for improved embryo selection in in vitro fertilization?

  • Embryo Biology
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Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

The present study evaluated the association between oxidative parameters in embryo cryopreservation medium and laboratory and clinical outcomes.

Methods

This prospective laboratory study was conducted in an IVF unit in a university-affiliated hospital with 91 IVF patients undergoing a frozen-thawed embryo transfer cycle. Following thawing, 50 μL of embryo cryopreservation medium was retrieved from each cryotube and tested by the thermochemiluminescence (TCL) assay. TCL amplitudes after 50 (H1), 150 (H2), and 280 s (H3) were recorded in counts per second (CPS) and the TCL ratio determined for comparison with implantation and pregnancy rates.

Results

A total of 194 embryos were transferred in 85 frozen-thaw cycles. Twenty-one pregnancies (24.7 %) occurred. Implantation and overall and clinical pregnancy rates were higher when the median TCL H1 amplitude was <32 CPS compared to ≥32 CPS (14.6 vs. 5.3 %, 37.5 vs. 17 %, 28.1 vs. 9.4 %, respectively). No pregnancies occurred when the H1 amplitude was ≥40 CPS. Logistic regression multivariate analysis found that only the median TCL H1 amplitude was associated with the occurrence of pregnancy (OR = 2.93, 95 % CI 1.065–8.08). The TCL ratio inversely correlated with the duration of embryo cryopreservation (r = −0.37).

Conclusions

The results indicate that thawed embryos may express oxidative processes in the cryopreservation medium, and higher oxidative levels are associated with lower implantation rates. These findings may aid in the improved selection of frozen-thawed embryos for IVF.

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Author information

Authors and Affiliations

  1. Fertility and IVF Unit, Department of Obstetrics and Gynecology, Carmel Medical Center, the Bruce Rappaport Faculty of Medicine, Technion, Israel Institute of Technology, 7 Michal St., Haifa, Israel

    Zofnat Wiener-Megnazi, Shirly Lahav-Baratz, Idit Blais, Sarah Matarasso, Mara Koifman, Sergei Shnizer, David Ishai, Gil Peer, Grace Younes, Ariel Zilberlicht, Ron Auslander & Martha Dirnfeld

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  1. Zofnat Wiener-Megnazi
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  2. Shirly Lahav-Baratz
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  3. Idit Blais
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  4. Sarah Matarasso
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  5. Mara Koifman
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  6. Sergei Shnizer
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  7. David Ishai
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Correspondence to Zofnat Wiener-Megnazi.

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The authors declare that they have no conflict of interest.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

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Capsule

An oxidative parameter in embryo cryopreservation medium as measured by thermochemiluminescence assay was associated with pregnancy in a prospective study. These findings may provide a tool for better embryo selection.

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Wiener-Megnazi, Z., Lahav-Baratz, S., Blais, I. et al. Oxidative markers in cryopreservation medium from frozen-thawed embryos: a possible tool for improved embryo selection in in vitro fertilization?. J Assist Reprod Genet 33, 731–739 (2016). https://doi.org/10.1007/s10815-016-0692-6

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  • DOI: https://doi.org/10.1007/s10815-016-0692-6

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