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Anti-Müllerian hormone gene polymorphism is associated with androgen levels in Chinese polycystic ovary syndrome patients with insulin resistance

  • Genetics
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

The objective of the study was to investigate whether genetic polymorphisms of the anti-Müllerian hormone (AMH) and its specific receptor anti-Müllerian hormone type II receptor (AMHRII) were associated with the hormone disorder and phenotype of polycystic ovary syndrome (PCOS).

Methods

This case-control study included 141 PCOS patients and 123 normal women. Two polymorphisms of AMH and AMHRII and the clinical characteristics of participants such as body mass index (BMI), serum luteinizing hormone (LH), estradiol levels (E2), total testosterone levels (T), and homeostasis model assessment of insulin resistance (HOMA-IR) were analyzed with the case-control sample. Gene–gene interactions of AMH and AMHRII genes were analyzed based multifactor-dimensionality reduction method.

Results

A significant difference of AMH gene polymorphisms were observed in IR-PCOS women and controls. The AMH and AMHRII gene polymorphisms were not found a significant difference in non-IR-PCOS and normal groups. To IR-PCOS women, genotypes of AMH were closely related to the serum levels of LH (P = 0.000), testosterone (P = 0.000) and HOMA-IR (P = 0.038), while in the non-IR-PCOS and normal groups, no relationship was found. No impact of AMH and AMHRII gene–gene interactions was demonstrated.

Conclusions

Our research suggests that the diversity of AMH genotypes in the AMH signal pathway may be connected with the susceptibility and phenotype of PCOS with insulin resistance.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 81260109/H0425), the Program for Special talents of Ningxia medical university (XT201421), the program of Ningxia Medical University (220–2001090105), the project on science and social development of Jiangsu (BE2012706), the Top Six Talent Peaks Program of Jiangsu (2014-WSW-080), and the National Science Foundation of Yangzhou (YZ2014050).

Authors’ contributions

Meng-Xue Zheng and Yan Li participated in study design, performed the experiments, and drafted the manuscript. Rong Hu participated in study design, directed the execution of experiments, and revised the article critically for intellectual content. Fei-Miao Wang participated in sample collection and performed statistical analysis. Xiao-Mei Zhang and Bing Guan participated in mentoring and critical discussion. All authors approved the final version of the manuscript.

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Author notes

    Authors and Affiliations

    1. Ningxia Medical University, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, 750004, China

      Meng-Xue Zheng

    2. Department of Gynecology, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, 750004, China

      Yan Li

    3. Reproductive Medicine Center, Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Ningxia Medical University, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, 750004, China

      Rong Hu & Fei-Miao Wang

    4. Reproductive Medicine Center, Department of Obstetrics and Gynecology, Northern Jiangsu People’s Hospital, Yangzhou University, Yangzhou, 225001, China

      Xiao-Mei Zhang & Bing Guan

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    1. Meng-Xue Zheng
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    Correspondence to Rong Hu.

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    Capsule

    AMH genetic polymorphism in the AMH signal pathway is related to serum androgen levels in Chinese PCOS with insulin resistance.

    Meng-Xue Zheng and Yan Li contributed equally to this work.

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    Zheng, MX., Li, Y., Hu, R. et al. Anti-Müllerian hormone gene polymorphism is associated with androgen levels in Chinese polycystic ovary syndrome patients with insulin resistance. J Assist Reprod Genet 33, 199–205 (2016). https://doi.org/10.1007/s10815-015-0641-9

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    • DOI: https://doi.org/10.1007/s10815-015-0641-9

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