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Does sperm origin affect embryo morphokinetic parameters?

  • Gamete Biology
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

The purpose of our study was to use time-lapse in order to evaluate the impact of sperm origin (fresh ejaculate or surgically retrieved) on embryo morphokinetic parameters and clinical outcome in intracytoplasmic sperm injection (ICSI) cycles.

Methods

This retrospective monocentric study was conducted in 485 unselected couples undergoing 604 ICSI cycles with embryo culture in the Embryoscope®. Among them, 445 couples underwent ICSI cycle with fresh ejaculated sperm and 40 with surgically retrieved sperm (26 with testicular sperm and 14 with epididymal sperm). Embryo morphokinetic parameters and clinical cycle outcome were compared between fresh ejaculated sperm and surgically retrieved sperm. A subgroup analysis was also conducted between testicular and epididymal sperm ICSI cycles.

Results

Clinical outcome was comparable between groups according to sperm origin. Although most early morphokinetic parameters were comparable between ejaculated and surgical sperm groups, a few parameters were significantly different between both groups, but with a considerable overlap in their distribution. Late cellular events occurred significantly later in the surgical sperm group than in the ejaculated sperm group.

Conclusions

Morphokinetic analysis did not allow us to identify clinically relevant differences between fresh ejaculate and surgically retrieved sperm groups. Further studies are needed, especially concerning the relationship between sperm origin and late morphokinetic parameters, such as blastocyst development.

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References

  1. Steptoe PC, Edwards RG. Birth after the reimplantation of a human embryo. Lancet. 1978;2:366.

    Article  CAS  PubMed  Google Scholar 

  2. Bromage SJ, Falconer DA, Lieberman BA, Sangar V, Payne SR. Sperm retrieval rates in subgroups of primary azoospermic males. Eur Urol. 2007;51:534–9.

    Article  PubMed  Google Scholar 

  3. Ben-Ami I, Raziel A, Strassburger D, Komarovsky D, Ron-El R, Friedler S. Intracytoplasmic sperm injection outcome of ejaculated versus extracted testicular spermatozoa in cryptozoospermic men. Fertil Steril. 2013;99:1867–71.

    Article  PubMed  Google Scholar 

  4. Desai N, AbdelHafez F, Sabanegh E, Goldfarb J. Paternal effect on genomic activation, clinical pregnancy and live birth rate after ICSI with cryopreserved epididymal versus testicular spermatozoa. Reprod Biol Endocrinol. 2009;7:142.

    Article  PubMed Central  PubMed  Google Scholar 

  5. Ishikawa T, Shiotani M, Izumi Y, Hashimoto H, Kokeguchi S, Goto S, et al. Fertilization and pregnancy using cryopreserved testicular sperm for intracytoplasmic sperm injection with azoospermia. Fertil Steril. 2009;92:174–9.

    Article  PubMed  Google Scholar 

  6. Tsai CC, Huang FJ, Wang LJ, Lin YJ, Kung FT, Hsieh CH, et al. Clinical outcomes and development of children born after intracytoplasmic sperm injection (ICSI) using extracted testicular sperm or ejaculated extreme severe oligo-astheno-teratozoospermia sperm: a comparative study. Fertil Steril. 2011;96:567–71.

    Article  PubMed  Google Scholar 

  7. van Loendersloot L, van Wely M, van der Veen F, Bossuyt P, Repping S. Selection of embryos for transfer in IVF: ranking embryos based on their implantation potential using morphological scoring. Reprod Biomed Online. 2014;29:222–30.

    Article  PubMed  Google Scholar 

  8. Paternot G, Wetzels AM, Thonon F, Vansteenbrugge A, Willemen D, Devroe J, et al. Intra- and interobserver analysis in the morphological assessment of early stage embryos during an IVF procedure: a multicentre study. Reprod Biol Endocrinol. 2011;9:127.

    Article  PubMed Central  PubMed  Google Scholar 

  9. Herrero J, Meseguer M. Selection of high potential embryos using time lapse imaging: the era of morphokinetics. Fertil Steril. 2013;99:1030–4.

    Article  PubMed  Google Scholar 

  10. Sundvall L, Ingerslev HJ, Breth Knudsen U, Kirkegaard K. Inter- and intra-observer variability of time-lapse annotations. Hum Reprod. 2013;28:3215–21.

    Article  PubMed  Google Scholar 

  11. WHO. Laboratory manual for the examination and processing of human semen. Fifthth ed. Geneva: World Health Organization; 2010.

    Google Scholar 

  12. Cedrin-Durnerin I, Guivarc'h-Leveque A, Hugues JN. Pretreatment with estrogen does not affect IVF-ICSI cycle outcome compared with no pretreatment in GnRH antagonist protocol: a prospective randomized trial. Fertil Steril. 2012;97:1359–64.

    Article  CAS  PubMed  Google Scholar 

  13. Ciray HN, Campbell A, Agerholm IE, Aguilar J, Chamayou S, Esbert M, et al. Proposed guidelines on the nomenclature and annotation of dynamic human embryo monitoring by a time-lapse user group. Hum Reprod. 2014;29:2650–60.

    Article  PubMed  Google Scholar 

  14. Hickman C, Lennon J, Cook C, Perez MJ, Mania A, Lavery S. Factors affecting morphokinetics: sperm origin, maternal age and polidy. Fertil Steril. 2013;100:S6–7.

    Article  Google Scholar 

  15. Chen AA, Tan L, Suraj V, Reijo Pera R, Shen S. Biomarkers identified with time-lapse imaging: discovery, validation, and practical application. Fertil Steril. 2013;99:1035–43.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  16. Kaser DJ, Racowsky C. Clinical outcomes following selection of human preimplantation embryos with time-lapse monitoring: a systematic review. Hum Reprod Update. 2014;20:617–31.

    Article  PubMed  Google Scholar 

  17. Meseguer M, Herrero J, Tejera A, Hilligsoe KM, Ramsing NB, Remohi J. The use of morphokinetics as a predictor of embryo implantation. Hum Reprod. 2011;26:2658–71.

    Article  PubMed  Google Scholar 

  18. Dal Canto M, Coticchio G, Mignini Renzini M, De Ponti E, Novara PV, Brambillasca F, et al. Cleavage kinetics analysis of human embryos predicts development to blastocyst and implantation. Reprod Biomed Online. 2012;25:474–80.

    Article  PubMed  Google Scholar 

  19. Fréour T, Le Fleuter N, Lammers J, Splingart C, Reignier A, Barrière P. External validation of a time-lapse prediction model. Fertil Steril. 2015;103:917–22.

    Article  PubMed  Google Scholar 

  20. Nilsson S, Waldenstrom U, Engstrom AB, Hellberg D. Single blastocyst transfer after ICSI from ejaculate spermatozoa, percutaneous epididymal sperm aspiration (PESA) or testicular sperm extraction (TESE). J Assist Reprod Genet. 2007;24:167–71.

    Article  PubMed Central  PubMed  Google Scholar 

  21. Braga DP, Setti AS, Vingris L, Figueira RC, Iaconelli A, Borges E. The male factor of infertility should not be an issue for the selection of patients for extended embryo culture programmes. Andrology. 2013;1:758–63.

    Article  CAS  PubMed  Google Scholar 

  22. Meseguer M, Rubio I, Cruz M, Basile N, Marcos J, Requena A. Embryo incubation and selection in a time-lapse monitoring system improves pregnancy outcome compared with a standard incubator: a retrospective cohort study. Fertil Steril. 2012;98:1481–9.

    Article  PubMed  Google Scholar 

  23. Oron G, Fisch B, Sapir O, Wertheimer A, Garor R, Feldberg D, et al. Pregnancy outcome after ICSI with thawed testicular sperm from men with non-obstructive azoospermia compared to ICSI with ejaculated sperm from men with severe oligoasthenoteratozoospermia and IVF with normal ejaculated sperm. Gynecol Endocrinol. 2014;30:103–6.

    Article  PubMed  Google Scholar 

  24. Xie D, Qiu Z, Luo C, Chu Q, Quan S. Effect of spermatozoa from different sources on normal fertilization of oocytes and embryo quality and development in intracytoplasmic sperm injection cycles. Nan Fang Yi Ke Da Xue Xue Bao. 2014;34:857–61.

    PubMed  Google Scholar 

  25. Amirjannati N, Heidari-Vala H, Akhondi MA, Hosseini Jadda SH, Kamali K, Sadeghi MR. Comparison of intracytoplasmic sperm injection outcomes between spermatozoa retrieved from testicular biopsy and from ejaculation in cryptozoospermic men. Andrologia. 2012;44 Suppl 1:704–9.

    Article  PubMed  Google Scholar 

  26. Bendikson KA, Neri QV, Takeuchi T, Toschi M, Schlegel PN, Rosenwaks Z, et al. The outcome of intracytoplasmic sperm injection using occasional spermatozoa in the ejaculate of men with spermatogenic failure. J Urol. 2008;180:1060–4.

    Article  PubMed  Google Scholar 

  27. Fedder J, Loft A, Parner ET, Rasmussen S, Pinborg A. Neonatal outcome and congenital malformations in children born after ICSI with testicular or epididymal sperm: a controlled national cohort study. Hum Reprod. 2013;28:230–40.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Service de Médecine et Biologie de la Reproduction, CHU de Nantes, Nantes, 44093, France

    Jenna Lammers, Arnaud Reignier, Carole Splingart, Aurore Catteau, Paul Barriere & Thomas Freour

  2. INSERM UMR 1064, ITERT, Nantes, France

    Jenna Lammers, Arnaud Reignier, Carole Splingart, Laurent David, Paul Barriere & Thomas Freour

  3. INSERM UMS 016, SFR Santé, iPSC Core Facility, Nantes, France

    Laurent David

  4. CNRS UMS 3556, SFR Santé, iPS Core Facility, Nantes, France

    Laurent David

  5. UFR Médecine, Université de Nantes, Nantes, France

    Arnaud Reignier, Laurent David, Paul Barriere & Thomas Freour

  6. ITUN, CHU Nantes, Nantes, France

    Jenna Lammers, Arnaud Reignier, Carole Splingart, Laurent David, Paul Barriere & Thomas Freour

  7. Clínica EUGIN, 08029, Barcelona, Spain

    Thomas Freour

Authors
  1. Jenna Lammers
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  2. Arnaud Reignier
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  3. Carole Splingart
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  4. Aurore Catteau
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  5. Laurent David
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  6. Paul Barriere
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  7. Thomas Freour
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Corresponding author

Correspondence to Thomas Freour.

Additional information

Capsule

Time-lapse parameters do not significantly differ according to sperm origin

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Lammers, J., Reignier, A., Splingart, C. et al. Does sperm origin affect embryo morphokinetic parameters?. J Assist Reprod Genet 32, 1325–1332 (2015). https://doi.org/10.1007/s10815-015-0517-z

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  • DOI: https://doi.org/10.1007/s10815-015-0517-z

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