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Short gamete co-incubation during in vitro fertilization decreases the fertilization rate and does not improve embryo quality: a prospective auto controlled study

  • Assisted Reproduction
  • Published:
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Abstract

Purpose

Evaluate the effect of short gamete incubation on fertilization rate and embryo quality.

Methods

A prospective study has been performed. Two thousand five hundred and forty seven sibling oocytes from 240 couples undergoing IVF attempts were allocated to a short (1 h) or a standard (18 h) insemination procedure. Diploid fertilization rate (two pronuclei, 2PN), polyspermy (>2PN) and embryo quality were compared.

Results

The fertilization rate was statistically lower in the short insemination group compared to the standard insemination one (64.9% and 70.1%; P = 0.039), with a similar polyspermy rate observed between the two groups. A slight, but non significant, increase was observed concerning good embryo quality rate in the short insemination group when compared to the standard insemination, both at day 2 (60.1 vs. 58.1%; P = 0.06) and day 3 (53.2 vs. 48.5%; P = 0.22).

Conclusion

This new study highlights that a 1 h gamete exposure decreases the fertilization rate and does not improve embryo quality compared with a standard 18 h insemination procedure.

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Authors and Affiliations

  1. Service d’Histologie Embryologie Cytogénétique, Hôpital Jean Verdier, Avenue du 14 Juillet, 93140, Bondy, France

    V. Barraud-Lange, C. Sifer, K. Pocaté, A. Ziyyat, B. Martin-Pont & J. P. Wolf

  2. Service de Biostatistique et d’Informatique Médicale, CHU Saint-Louis, 75475, Paris cedex 10, France

    R. Porcher

  3. Service de Médecine de la Reproduction, Hôpital Jean Verdier, 93140, Bondy, France

    J. N. Hugues

Authors
  1. V. Barraud-Lange
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  2. C. Sifer
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  3. K. Pocaté
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  4. A. Ziyyat
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  5. B. Martin-Pont
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  6. R. Porcher
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  7. J. N. Hugues
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  8. J. P. Wolf
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Corresponding author

Correspondence to V. Barraud-Lange.

Additional information

Capsule This study highlights that a 1 h gamete exposure decreases the fertilization rate and does not improve embryo quality compared with a standard 18 h insemination procedure.

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Barraud-Lange, V., Sifer, C., Pocaté, K. et al. Short gamete co-incubation during in vitro fertilization decreases the fertilization rate and does not improve embryo quality: a prospective auto controlled study. J Assist Reprod Genet 25, 305–310 (2008). https://doi.org/10.1007/s10815-008-9240-3

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  • DOI: https://doi.org/10.1007/s10815-008-9240-3

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