Abstract
Purpose
To assess the ability of fibroblasts isolated from normal peritoneum and adhesion tissues to express various hormone receptors when cultured with exogenous estradiol.
Methods
Primary cultures of fibroblasts from normal human peritoneum and adhesion tissue were treated with zero (control), 10−10, 10−8, and 10−6 M concentrations of 17β-estradiol. We performed real time reverse transcriptase polymerase chain reaction to determine mRNA levels of estradiol-α receptor (ER-α) and estradiol-β receptor (ER-β), progesterone receptor (P-R), androgen receptor (A-R), and prolactin receptor (PRL-R) in the two types of fibroblast cultures.
Results
In the control groups, P-R and A-R were higher in normal than in adhesion fibroblasts. In adhesion cells, ER-α were higher at 10−8 estradiol; ER-β were higher at 10−6 M estradiol; P-R remained constant; A-R showed a higher expression at 10−10 and 10−8 M estradiol; and PRL-R showed an exponential increase at 10−10 M estradiol.
Conclusions
The inflammatory-like changes manifested by adhesion fibroblasts enhance the anabolic hormones receptor expression (ER-α, ER-β, PRL, and A-R), when exposed to estradiol.
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References
Wiczyk HP, Grow DR, Adams LA, O’Shea DL, Reece MT. Pelvic adhesions contain sex steroid receptors and produce angiogenesis growth factors. Fertil Steril 1998;69:511–6.
Diamond MP, DeCherney AH. Pathogenesis of adhesion formation/reformation: application to reproductive pelvic surgery. Microsurgery 1987;8:103–7.
Saed GM, Zhang W, Diamond MP. Molecular characterization of fibroblasts isolated from human peritoneum and adhesions. Fertil Steril 2001;75:763–8.
Henson MC, Shi W, Greene SJ, Reggio BC. Effects of pregnant human, non-pregnant human, and fetal bovine sera on human chorionic gonadotropin, estradiol, and progesterone release by cultured human trophoblast cells. Endocrinology 1996;137:2067–74.
Saed GM, Zhang W, Chegini N, Holmdahl L, Diamond MP. Alteration of type I and III collagen expression in human peritoneal mesothelial cells in response to hypoxia and transforming growth factor-beta1. Wound Repair Regen 1999;7:504–10.
Maurer JH, Bonaventura LM. The effect of aqueous progesterone on operative adhesion formation. Fertil Steril 1983;39:485–9.
Montanino-Oliva M, Metzger DA, Luciano AA. Use of medroxyprogesterone acetate in the prevention of postoperative adhesions. Fertil Steril 1996;65:650–4.
Frazier-Jessen MR, Mott FJ, Witte PL, Kovacs EJ. Estrogen suppression of connective tissue deposition in a murine model of peritoneal adhesion formation. J Immunol 1996;156:3036–42.
Wright JA, Sharpe-Timms KL. Gonadotropin-releasing hormone agonist therapy reduces postoperative adhesion formation and reformation after adhesiolysis in rat models for adhesion formation and endometriosis. Fertil Steril 1995;63:1094–100.
Grow DR, Coddington CC, Hsiu JG, Mikich Y, Hodgen GD. Role of hypoestrogenism or sex steroid antagonism in adhesion formation after myometrial surgery in primates. Fertil Steril 1996;66:140–7.
Sharpe-Timms KL, Zimmer RL, Jolliff WJ, Wright JA, Nothnick WB, Curry TE. Gonadotropin-releasing hormone agonist (GnRH-a) therapy alters activity of plasminogen activators, matrix metalloproteinases, and their inhibitors in rat models for adhesion formation and endometriosis: potential GnRH-a-regulated mechanisms reducing adhesion formation. Fertil Steril 1998;69:916–23.
Ergeneli MH, Kuscu E, Zeyneloglu HB, Haberal A, Erdogan M. GnRHa for adhesion formation after uterine serosal injury in an euestrogenic milieu. J Reprod Med 2000;45:35–8.
Freeman ML, Saed GM, Diamond MP. Hormone-independent ovarian influence on adhesion development. Fertil Steril 2002;78:340–6.
Henzl MR, Corson SL, Moghissi K, Buttram VC, Berqvist C, Jacobson J. Administration of nasal nafarelin as compared with oral danazol for endometriosis. A multicenter double-blind comparative clinical trial. N Engl J Med 1988;318:485–9.
Kuhlmann M, Gartner A, Schindler EM, Regidor PA, Buhler K, Schindler AE. Uterine leiomyomata and sterility: therapy with gonadotropin-releasing hormone agonists and leiomyomectomy. Gynecol Endocrinol 1997;11:169–74.
Canbaz MA, Ustun C, Kocak I, Yanik FF. The comparison of gonadotropin-releasing hormone agonist therapy and intraperitoneal Ringer’s lactate solution in prevention of postoperative adhesion formation in rat models. Eur J Obstet Gynecol Reprod Biol 1999;82:219–22.
Roberts JE, Watkin KL, Tulandi T. Effects of leuprolide acetate on adhesion formation and wound healing in the rat model. J Am Assoc Gynecol Laparosc 2001;8:124–8.
Ben-Jonathan N, Mershon JL, Allen DL, Steinmetz RW. Extrapituitary prolactin: distribution, regulation, functions, and clinical aspects. Endocr Rev 1996;17:639–69.
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When adhesion, but not normal, fibroblasts are exposed to increasing estradiol concentrations, they show enhanced expression of anabolic hormone receptors (ER-α, ER-β, PRL, and A-R).
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Detti, L., Saed, G.M., Jiang, Z.L. et al. The effect of estradiol on the expression of estrogen, progesterone, androgen, and prolactin receptors in human peritoneal fibroblasts. J Assist Reprod Genet 25, 245–250 (2008). https://doi.org/10.1007/s10815-008-9230-5
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DOI: https://doi.org/10.1007/s10815-008-9230-5