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Developmental potential of isolated blastomeres from early mouse embryos in the presence and absence of LIF and GM-CSF

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Abstract

Purpose

The aim of this study was to investigate the developmental potential of isolated blastomeres in the presence and absence of leukemia inhibitory factor (LIF) and granulocyte–macrophage colony stimulating factor (GM-CSF).

Methods

The blastomeres of two (1/2) and eight cells (1/8) embryos were isolated and cultured in T6 medium in the presence and absence of LIF (1,000 IU/ml) and or GM-CSF (2 ng/ml) up to 120 h. The diameter and cell number of blastocysts were measured.

Results

The developmental rates of 1/2 isolated blastomeres developed to blastocysts stages in the presence and absence of LIF and GM-CSF were 45.80, 35.10 and 48.66, 41.66, respectively. The diameter of blastocysts was higher in GM-CSF group and total cell number of blastocyst in both treated groups was higher than control (P < 0.05). No 1/8 blastomeres developed to morula and blastocyst stages.

Conclusions

LIF and GM-CSF could improve the development of 1/2 isolated blastomeres.

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Acknowledgment

The authors would like to thank Miss Ebrahimi for photographic art work and Mr Pourbyranvand for technical assistance.

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Authors and Affiliations

  1. Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, P.O. Box: 14115-111, Tehran, Iran

    Behnaz Sheikholslami, Mojdeh Salehnia, Mojtaba Rezazadeh Valojerdi & Mehdi Ramezanzadeh

Authors
  1. Behnaz Sheikholslami
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  2. Mojdeh Salehnia
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  3. Mojtaba Rezazadeh Valojerdi
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  4. Mehdi Ramezanzadeh
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Corresponding author

Correspondence to Mojdeh Salehnia.

Additional information

Capsule GM-CSF improved the developmental potential and the quality of isolated blastomeres derived from mouse two cells embryo.

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Sheikholslami, B., Salehnia, M., Valojerdi, M.R. et al. Developmental potential of isolated blastomeres from early mouse embryos in the presence and absence of LIF and GM-CSF. J Assist Reprod Genet 25, 7–12 (2008). https://doi.org/10.1007/s10815-007-9191-0

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  • DOI: https://doi.org/10.1007/s10815-007-9191-0

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