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Effects of Coculture With Immune Cells on the Developmental Competence of Mouse Preimplantation Embryos in Vitro and in Utero

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Abstract

The aim of this study was to establish a coculture system using immune cells as well as an in vitro model for inflammatory conditioning using RAW 264.7 mouse macrophages activated by lipopolysaccharide. The direct and indirect coculture systems were applied to evaluate the influence of embryo-to-cell direct or indirect secretory molecules from the cocultured cells. Blastulation rate in vitro (94.6% vs 76.9%, P < .05) and implantation rate in utero (43.3% vs 17.6%, P < .01) were significantly increased in direct coculture with activated RAW 264.7 cells compared to control. We also found the embryotrophic effects in vitro in the indirect coculture system. Our results indicate that coculture of mouse preimplantation embryos with immune cells could improve the developmental competence in vitro and in utero. Taken together, RAW 264.7 cells secret embryotrophic molecules, and it suggests the valuable insights that immune cells could improve in vitro culture conditions of preimplantation embryos.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam, Korea

    Jaewang Lee MS

  2. Seoul National University College of Medicine, Seoul, Korea

    Jaewang Lee MS & Seok Hyun Kim MD, PhD

  3. Department of Senior Healthcare, BK21 plus Program, Graduated School, Eulji University, Korea

    Jihyun Kim MS, Hee-Gyoo Kang PhD & Jin Hyun Jun PhD

  4. Department of Obstetrics and Gynecology, Seoul National University Hospital, Seoul, Korea

    Seok Hyun Kim MD, PhD

  5. Department of Biomedical Laboratory Science, Graduate School of Health Science, Eulji University, Seongnam, Korea

    Hee-Gyoo Kang PhD & Jin Hyun Jun PhD

  6. Eulji Medi-Bio Research Institute (EMBRI), Eulji University, Korea

    Jin Hyun Jun PhD

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  1. Jaewang Lee MS
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Correspondence to Jin Hyun Jun PhD.

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Lee, J., Kim, J., Kim, S.H. et al. Effects of Coculture With Immune Cells on the Developmental Competence of Mouse Preimplantation Embryos in Vitro and in Utero. Reprod. Sci. 22, 1252–1261 (2015). https://doi.org/10.1177/1933719115574342

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