We propose a fast method for determining the surface area and volume of erythrocytes in the biconcave-discoid shape, based on establishing regression relations between the parameters to be determined and the angular polarization coefficients P(Θ) and scattering indicatrix σ(Θ). We have shown that using σ(Θ) for angles 6° and 17°, P(Θ) for angles 81° and 119° lets us determine the surface area of the erythrocytes within ≈1% and the volume of the erythrocytes within ≈2%.
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Translated from Zhurnal Prikladnoi Spektroskopii, Vol. 82, No. 6, pp. 909–915, November–December, 2015.
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Kugeiko, M.M., Smunev, D.A. Method for Determining Erythrocyte Surface Area by Polarization and Nephelometric Measurements. J Appl Spectrosc 82, 985–992 (2016). https://doi.org/10.1007/s10812-016-0216-2
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DOI: https://doi.org/10.1007/s10812-016-0216-2