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Simple molecular identification and free amino acid contents of Neoporphyra kitoi (Bangiales, Rhodophyta) with potential use as a new species for nori cultivation

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Abstract

We conducted preliminary experimental cultivation using the OHN-1 strain of Neoporphyra kitoi to confirm the growth of the gametophytic blades in the nori farm. The mature and immature long linear blades grew in the nori farm and were confirmed to be N. kitoi based on the nucleotide sequences of the chloroplast RUBISCO spacer region. Furthermore, we developed PCR–RFLP analyses using chloroplast and nuclear DNA to discriminate simply between N. kitoi and Neopyropia yezoensis, the species cultivated in Japan, for identifying many samples for growth comparisons between the two species during future experimental cultivation. In addition, four major FAA contents [alanine (Ala), aspartic acid (Asp), glutamic acid (Glu) and taurine (Tau)] were compared between the blades of N. kitoi OHN-1 and cultivated Neopy. yezoensis (Neopyropia yezoensis f. narawaensis) HG-4 under identical laboratory conditions. As a result, the contents of the four major FAAs, especially Ala, were significantly higher in OHN-1 than in HG-4. The results suggested that the OHN-1 strain of N. kitoi has potential as a new cultivated species with high growth and a better flavor.

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Data availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

Notes

  1. A recent paper by Zuccarello et al (2022) has proposed that the genera Neopyropia and Neoporphyra (Yang et al 2020) are synonyms of Pyropia sensu Sutherland et al (2011).

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Acknowledgements

We thank the members of our laboratory for helping with the laboratory work. This work was supported by JSPS KAKENHI Grant Number 22H02418 to K. N. and by the Japan Fisheries Research and Education Agency.

Funding

This study was funded by the Japan Society for the Promotion of Science (JSPS), KAKENHI Grant Number 22H02418 (Kyosuke Niwa).

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Authors and Affiliations

  1. Department of Marine Biosciences, Tokyo University of Marine Science and Technology, Tokyo, Minato, 108‐8477, Japan

    Kyosuke Niwa & Hiroshi Iga

  2. Tokyo Bay Fisheries Laboratory, Chiba Prefecture Fisheries Research Center, Chiba, 293-0042, Japan

    Yushi Shimada

  3. Nansei Field Station, Fisheries Technology Institute, Japan Fisheries Research and Education Agency, 422-1 Nakatsuhamaura, Minami-Ise, Mie, 516-0193, Japan

    Hirofumi Furuita

  4. Tamaki Field Station, Fisheries Technology Institute, Japan Fisheries Research and Education Agency, 224-1 Hiruda, Tamaki, Mie, 519-0423, Japan

    Takeshi Yamamoto

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  1. Kyosuke Niwa
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  2. Hiroshi Iga
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  3. Yushi Shimada
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  4. Hirofumi Furuita
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  5. Takeshi Yamamoto
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Contributions

KN; Conceptualization, Methodology, Investigation, Writing-Original draft and Review & Editing. HI; Investigation; Formal analysis. YS; Investigation. HF; Formal analysis. TY; Investigation, Formal analysis.

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Correspondence to Kyosuke Niwa.

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Niwa, K., Iga, H., Shimada, Y. et al. Simple molecular identification and free amino acid contents of Neoporphyra kitoi (Bangiales, Rhodophyta) with potential use as a new species for nori cultivation. J Appl Phycol 35, 331–338 (2023). https://doi.org/10.1007/s10811-022-02896-8

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  • DOI: https://doi.org/10.1007/s10811-022-02896-8

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