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Cultivation of Osmundea pinnatifida (Hudson) Stackhouse in the Algem® photobioreactor system

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Abstract

Cultivation of Osmundea pinnatifida (Hudson) Stackhouse in flasks and/or tanks has yet to be developed beyond the lab scale. However, establishment of a methodology for the supply of this red seaweed has great potential, considering that it can be exploited either as food or as a source of biologically active compounds with potential nutraceutical, pharmaceutical and cosmetic applications. This study investigates the possibility of growing O. pinnatifida in the Algem® photo bioreactor (PBR) system, normally utilised for microalgae cultivation, and examines the antioxidant content of the biomass. The cultures were incubated in the Algem® PBRs under a set of environmental parameters established in a previous seasonality study at a single location on the west coast of Scotland. The growth of the cultures in the PBR was monitored, the biomass assessed for antioxidant content, and results compared with seasonal samples, to assess how the PBR system affects the biochemistry of this species. Analyses centred on antioxidant activity and included Total Phenolic Content (TPC) and Ferric Iron Reducing Antioxidant Power (FRAP). A significant increase in the antioxidant content, two to five-time higher compared to wild samples, was achieved by cultivating the biomass in the PBR. This study highlights that the production of antioxidant compounds in O. pinnatifida can be improved by increasing photoperiod and light intensity and manipulating the wavelength. This information provides important insights into how cultivation conditions for this species can be tailored to increase production and improve the composition of the product of interest.

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Acknowledgements

This research was part of a Ph.D. project awarded by the University of Aberdeen, with The Scottish Association for Marine Science (SAMS), the University of the Highlands and Islands (UHI), and the James Hutton Institute as academic partners.

Funding

This research was part of a Ph.D. project with the financial support from the Industrial Biotechnology Innovation Centre (IBioIC), Highlands and Islands Enterprise (HIE) and Marine Alliance for Science and Technology for Scotland (MASTS).

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Author notes

  1. Cecilia Biancacci

    Present address: School of Life and Environmental Sciences, Centre for Integrative Ecology, Deakin University, Warrnambool Campus, P.O. Box 423, Warrnambool, VIC, 3280, Australia

Authors and Affiliations

  1. Scottish Association for Marine Sciences, Oban, Scotland, UK

    Cecilia Biancacci, J. G. Day & M. S. Stanley

  2. The James Hutton Institute (Plant Biochemistry and Food Quality Group, Environmental and Biochemical Sciences Department), Invergowrie, Scotland, UK

    G. J. McDougall

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  1. Cecilia Biancacci
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  2. G. J. McDougall
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  3. J. G. Day
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  4. M. S. Stanley
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Contributions

Cecilia Biancacci: Conceptualization, Methodology, Validation, Formal analyses, Investigation, Writing—Original Draft, Writing—Review & Editing, Visualization Gordon J. McDougall: Writing—Review & Editing, Supervision, Resources John G. Day: Writing—Review & Editing, Supervision, Resources Michele S. Stanley: Conceptualization, Writing—Review & Editing, Supervision, Resources, Project Administration, Funding acquisition.

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Correspondence to Cecilia Biancacci.

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Biancacci, C., McDougall, G.J., Day, J.G. et al. Cultivation of Osmundea pinnatifida (Hudson) Stackhouse in the Algem® photobioreactor system. J Appl Phycol 34, 3095–3105 (2022). https://doi.org/10.1007/s10811-022-02837-5

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