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Single and mixed species of microalgae as larval food for the tropical sea cucumber Holothuria scabra

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Abstract

Different microalgae as larval food for Holothuria scabra, a high-value sea cucumber species with hatchery technology continually being refined to improve production, were studied. Two experiments were conducted to determine the performance of the larvae—first, the use of single-algal diet of either Chaetoceros calcitrans, Rhodomonas sp., or Tetraselmis tetrathele as feed; and second, the use of a combination of two algal species, C. calcitrans + Rhodomonas sp., C. calcitrans + T. tetrathele, or Rhodomonas sp. + T. tetrathele as feed. For both experiments, hatched larvae at 2 days post-fertilization (DPF) were used for initial stocking at a density of 200 larvae L−1. Growth, development, and survival were monitored until more than 50% of the larval population were considered as competent larvae (late auricularia). In the first experiment, during the early stage of development (early aricularia), larvae fed Rhodomonas sp. were larger compared to other treatments. However, when the larvae reached middle and late auricularia, they performed better when fed with C. calcitrans. Those larvae fed with C. calcitrans were significantly larger and showed faster development to competent larvae. In the second experiment, larvae fed mixed C. calcitrans + Rhodomonas sp. showed better performance in growth, development, and survival. The present study showed that C. calcitrans, when used solely as feed or in combination with Rhodomonas sp., promoted better growth, development, and survival of H. scabra larvae.

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The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

The authors would like to thank the Southeast Asian Fisheries Development Center, Aquaculture Department, for the use of facilities and for providing the microalgae and H. scabra larvae during the conduct of the experiments; Mr. Neil Tibubos, Mr. Jesus Rodriguez Jr., and Mr. Romeo Tibudan for their kind assistance during the conduct of the experiments; and the anonymous journal reviewers for patiently reviewing the manuscript.

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Author notes

  1. Fiona L. Pedroso

    Present address: Mindanao State University-Naawan, 9023, Naawan, Misamis Oriental, Philippines

Authors and Affiliations

  1. Institute of Aquaculture, College of Fisheries and Ocean Sciences, University of the Philippines Visayas, 5023, Miagao, Iloilo, Philippines

    Rema C. Sibonga, Liberato V. Laureta & Fiona L. Pedroso

  2. Aquaculture Department, Southeast Asian Fisheries Development Center, 5021, Tigbauan, Iloilo, Philippines

    Rema C. Sibonga & Ma. Junemie Hazel Lebata-Ramos

  3. Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas, 5023, Miagao, Iloilo, Philippines

    Marie Frances J. Nievales

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  1. Rema C. Sibonga
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Correspondence to Rema C. Sibonga.

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Sibonga, R.C., Laureta, L.V., Lebata-Ramos, M.J.H. et al. Single and mixed species of microalgae as larval food for the tropical sea cucumber Holothuria scabra. J Appl Phycol 33, 3103–3112 (2021). https://doi.org/10.1007/s10811-021-02512-1

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