Abstract
Gelidium floridanum is a red seaweed of economic importance as it yields high-quality agar which is extracted exclusively from material harvested from natural beds. Phytoregulators have not been explored in macroalgal culture as a probable alternative for large-scale farming of seaweed. Therefore, this study aimed to analyze the direct regeneration of G. floridanum explants using indole-3-acetic acid (IAA), jasmonic acid (JA), and gibberellic acid (GA). Explants (5 mm) were treated in liquid medium supplemented with IAA (0.57, 5.7, and 57 μM), JA (2, 4, and 8 mM), and GA (29, 43, and 58 mM) for 48 h and then further cultivated for 20 days. To observe the morphology and formation of new axes, micrographs were taken on the 5th day of culture using stereoscopic microscopy, and explants were processed for light and scanning electron microscopy. On the 20th day micrographs were obtained through stereoscopic microscopy for a final count of formed axes. After 5 days the formation of upright axes was greater and with a greater average size in the 2 and 4 mM JA treatments and floridean starch grains accumulated at the base, not being observed in the apical region. After 20 days the formation of new explants was higher in all treatments with IAA. We conclude that the direct regeneration of G. floridanum explants occurs from the medullary cells and starts in the first week of culture. Among the phytoregulators tested, JA contributed to the earlier formation of the upright axes, but after 20 days, IAA proved to be more efficient in the formation and size of these axes.
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Batista, D., Pereira, D.T., Carvalho, M.F. et al. Effects of indole-3-acetic acid (IAA), jasmonic acid (JA), and gibberellic acid (GA3) on the direct regeneration of Gelidium floridanum explants. J Appl Phycol 33, 1089–1099 (2021). https://doi.org/10.1007/s10811-020-02344-5
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DOI: https://doi.org/10.1007/s10811-020-02344-5