Abstract
Laboratory culture experiments were performed to optimize conditions for scaled-up production of Monostroma sp. The effects of growth determining factors like salinity (15–45 psu), photoperiod (8:16–16:8 L/D), light intensity (2–60 μmol photons m−2 s−1), and temperature (15–35 °C) on the growth of Monostroma sp. were assessed. Culture results indicated high tolerance capacity of Monostroma sp. to all levels in salinity, photoperiod, light intensity and temperature tested. ANOVA results confirmed significant impact of salinity, photoperiod, light intensity and temperature on growth rate (p < 0.05). The maximum growth rate was observed in the range of 5.73 to 14.41% day−1 which obtained in 35 psu salinity, 25 °C temperature, 14:10 (L/D) photoperiod, 60 μmol photons m−2 s−1 light intensity level and 1/4 MP1 medium. The modified 1/4MP1 medium was found to be suitable for enhanced growth of Monostroma sp. Outdoor tank culture with 1/4 MP1 medium showed maximum daily growth rate (14.38 ± 0.32% day−1).
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Acknowledgments
The authors expressed their sincere thanks to Dr. Amitava Das, Director, CSMCRI for his encouragement to pursue this work. The authors expressed their sincere thanks to Dr. C.R.K. Reddy, former Divisional Chair, Discipline of Marine Biotechnology and Ecology, CSMCRI for his keen interest in the work. We also thank Dr. P. K. Agarwal, Divisional Chair, Biotechnology and Phycology Division, CSMCRI for providing facilities. The authors are grateful to Dr. V. A. Mantri Group Head, Division of Biotechnology and Phycology for his valuable suggestions while writing this manuscript. This contribution has CSIR-CSMCRI PRIS registration number (CSIR-CSMCRI-211/2019).
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The financial support by the Council of Scientific and Industrial Research (CSIR), New Delhi under in-house project (MLP022) is gratefully acknowledged.
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Research Highlights
1. Monostroma species is showing augmented growth in natural environmental conditions than controlled laboratory conditions.
2. Monostroma species is an ideal candidate for scaled-up production in tank in India.
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Kavale, M.G., Italiya, B. & Veeragurunathan, V. Scaling the production of Monostroma sp. by optimizing culture conditions. J Appl Phycol 32, 451–457 (2020). https://doi.org/10.1007/s10811-019-01922-6
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DOI: https://doi.org/10.1007/s10811-019-01922-6