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Improvement of photobiological hydrogen production by suspended and immobilized cells of the N2-fixing cyanobacterium Fischerella muscicola TISTR 8215

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Abstract

To develop H2 photoproduction by using the N2-fixing cyanobacterium Fischerella muscicola TISTR 8215, a novel strain isolated from soil in Thailand, the factors affecting H2 production were investigated in this study. Enhanced H2 production in suspension culture was obtained when adapting the cells under N2-fixing condition (modified AA medium) with continuous illumination of 250 μmol photons m−2 s−1 under aerobic condition for 24 h, followed by further incubation under anaerobic condition for 9 h for production phase. The maximum H2 production rate was 38.5 μmol mg−1 chl a h−1. Low concentration of Fe2+ and Mo6+, essential elements for nitrogenase, enhanced H2 production. The enhanced H2 production was accompanied by the upregulation of nifD. On the other hand, an increased hupL transcript level was observed when there was a decrease of H2 production. In cells immobilization, 1.5% (w/v) agar-immobilized cells had a 23-fold increase in maximum H2 yield compared with that using free cell suspension at the same cell concentration, i.e., 7.48 mmol H2 L−1 by immobilized cells and 0.32 mmol H2 L−1 by suspended cells. Moreover, cell immobilization in agar could prolong H2 production up to 108 h. This study underlines the strategies toward enhanced and sustained H2 production from cyanobacteria. Furthermore, it will pave the way for large-scale production of biohydrogen to be used as an eco-friendly energy resource.

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Funding

This work was financially supported by Chulalongkorn University Dutsadi Phiphat Scholarship and the 90th Anniversary of Chulalongkorn University Fund (Ratchadaphiseksomphote Endowment Fund) to P. Wutthithien and A. Incharoensakdi.

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Authors and Affiliations

  1. Laboratory of Cyanobacterial Biotechnology, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand

    Palaya Wutthithien & Aran Incharoensakdi

  2. Program of Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand

    Palaya Wutthithien

  3. Microbial Chemistry, Department of Chemistry – Angstrom Laboratory, Uppsala University, Box 523, SE-75120, Uppsala, Sweden

    Peter Lindblad

  4. Academy of Science, Royal Society of Thailand, Bangkok 10300, Thailand

    Aran Incharoensakdi

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  1. Palaya Wutthithien
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  2. Peter Lindblad
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  3. Aran Incharoensakdi
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Correspondence to Aran Incharoensakdi.

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Wutthithien, P., Lindblad, P. & Incharoensakdi, A. Improvement of photobiological hydrogen production by suspended and immobilized cells of the N2-fixing cyanobacterium Fischerella muscicola TISTR 8215. J Appl Phycol 31, 3527–3536 (2019). https://doi.org/10.1007/s10811-019-01881-y

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