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Isolation, identification, and algicidal activity of marine bacteria against Cochlodinium polykrikoides

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Abstract

The aim of this study was to isolate and identify algicidal bacteria against the dinoflagellate Cochlodinium polykrikoides, and to determine the algicidal activity and algicidal range. During the declining period of C. polykrikoides blooms, seven algicidal bacteria were isolated. The algicidal bacteria against C. polykrikoides were enumerated using the most probable number (MPN) method. The number of algicidal bacteria was high (3.7 × 103 mL−1). Algicidal bacteria were identified on the basis of biochemical and chemotaxonomic characteristics, and analysis of 16S rDNA sequences. Seven algicidal bacteria isolated in this study belonged to the genera Bacillus, Dietzia, Janibacter, and Micrococcus. The most algicidal bacterium, designated Micrococcus luteus SY-13, is assumed to produce secondary metabolites. When 5% culture filtrate of this strain was applied to C. polykrikoides cultures, over 90% of C. polykrikoides cells were destroyed within 6 h. M. luteus SY-13 showed significant algicidal activities against C. polykrikoides and a wide algicidal range against various harmful algal bloom (HAB) species. Taken together, our results suggest that M. luteus SY-13 could be a candidate for controlling HABs.

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Acknowledgments

We would like to express our appreciation to the Department of Harmful Organisms of NFRDI (National Fisheries Research and Development Institute, Republic of Korea) for providing algal cultures. Thanks are extended to the South Sea Institute of KORDI (Korea Ocean Research & Development Institute, Republic of Korea) for providing additional dinoflagellate cultures.

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Authors and Affiliations

  1. Department of Microbiology, College of Natural Sciences, Pusan National University, Busan, 609-735, South Korea

    Min-Ju Kim & Sang-Joon Lee

  2. Korea Bio-IT Foundry Center, Pusan National University, Busan, 609-735, South Korea

    Seong-Yun Jeong

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  1. Min-Ju Kim
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Correspondence to Sang-Joon Lee.

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Kim, MJ., Jeong, SY. & Lee, SJ. Isolation, identification, and algicidal activity of marine bacteria against Cochlodinium polykrikoides . J Appl Phycol 20, 1069–1078 (2008). https://doi.org/10.1007/s10811-008-9312-x

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  • DOI: https://doi.org/10.1007/s10811-008-9312-x

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