Abstract
A type of growth inhibitor was successfully isolated and purified from cell-free filtrates of cultural medium at the death phase of Isochrysis galbana, and its chemical structure was confirmed by the methods of FABMS, UV, 1H-NMR, 13C-NMR and 2D NMR, which was 1-[hydroxyl-diethyl malonate]-isopropyl dodecenoic acid, C22H38O7. The results showed that the growth-inhibitor strongly inhibited the growth of Isochrysis galbana, and the growth of the eight species of microalgae (Dunaliella salina, Platymonas elliptica, Chlorella vugralis, Nitzschia closterium, Chaetoceros muelleri, Chaetoceros gracilis, Nitzschia closterium minutissima, Phaeodactylum tricornutum) also could be regulated by the growth-inhibitor in a concentration-dependent manner. The further investigation found that the synthesis process of chlorophyll and protein in the cells of all test microalgae could be inhibited by the growth inhibitor, and the content of chlorophyll and protein significantly decreased.
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References
Arzul G, Seguel M, Guzman L, Erard-Le Denn E (1999) Comparison of allelopathic properties in three toxic Alexandrium species. J Exp Marine Bio Ecol 232:285–295
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Doan NT, Rickards RW, Rothschild JM, Smith GD (2000) Allelopathicactions of the alkaloid 12-epi-hapalindole E isonitrile and calothrixin A from cyanobacteria of the genera Fischerella and Calothrix. J Appl Phycol 12:409–416
Ervin GN, Wetzel RG (2000) Allelochemical autotoxicity in the emergent wetland macrophyte Juncus effusus (Juncathe crude ethyl acetate extract). Am J Bot 87:853–860
Fogg GE (1966) The extracellular products of algae. Oceanogr Mar Biol Ann Rev 4:195–212
Guillard RRL, Ryther JH (1962) Studies of marine planktonic diatom. I. Cyclotella nana Hustedt and Detonula confervacea (Cleve) Gran. Can J Microbiol 17:309–314
Imada N, Kobayashi K, Tahara K, Oshima Y (1991) Production of an Autoinhibitor by Skeletonema costatum and its effect on the growth of other phytoplankton. Nippon Suisan Gakkaishi 57:2285–2290
Imada N, Kobayashi K, Isomura K, Saito H, Kimura S, Tahara K, Oshima Y (1992) Isolation and identification of an autoinhibitor produced by Skeletonema costatum. Nippon Suisan Gakkaishi 58:1687–1692
Jensen A (1978) Handbook of physiological methods. Cambridge University Press, New York
Kaplan D, Richmond AE, Dubinsky Z, Aaronson S (1986) Algal nutrition. In: Richmond A (ed) Handbook of microalgal mass culture. CRC, Boca Raton, FL, pp 147–160
Kearns KD, Hunter MD (2000) Green algal extracellular production in cyanobacterium. Environ Microbiol 2:291–297
Hellebust JA (1965) Excretion of some organic compounds by marine phytoplankton. Limnol Oceanogr 10:192–206
Hellebust JA (1974) Extracellular products. In: Stewart WDP (ed) Algal physiology and biochemistry. University of California Press, Oxford, pp 838–863
Perez E, Martin DE, Padilla M (1999) Rate of production of APONINs by Nannochloris oculata. Biomed Lett 59:88–91
Pflugmacher S (2002) Possible allelopathic effects of cyanotoxins, with reference to microcystin-LR, in aquatic ecosystems. Environ Toxicol 17:407–413
Pratt R (1942) Studies on Chlorella vulgaris. V some properties of the growth-inhibitor formed by Chlorella cells. Am J Bot 29:142–148
Regan DL, Ivancic N (1984) Mixed population of marine microalgae in continuous culture: factors affecting species dominance and biomass productivity. Biotechnol Bioeng 26:1265–1271
Sukenik A, Wahnon R (1991) Biochemical quality of marine unicellular algae with special emphasis on lipid composition: I. Isochrysis galbana. Aquaculture 97:61–72
Sun YN, Yin MY, Liu JG (2001) Auto-signals in Haematococcus pluvialis. Transactions of Oceanology and Limnology 3:22–28
Wang CH, Sun YY (2006) Initial researches on the growth inhibitor in culture of Isochrysis galbana. Marine Sci Bull 25(3):29–33
Wu TT, Kuo-Huang LL, Lee J (1998) Algicidal effect of Peridinium bipes on Microcystis aeruginosa. Current Micobio 37:256–261
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This work was supported by the key project of the National 10th Five-Year Plan Program (2001BA707B03).
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Yingying, S., Changhai, W. & Jing, C. Growth inhibition of the eight species of microalgae by growth inhibitor from the culture of Isochrysis galbana and its isolation and identification. J Appl Phycol 20, 315–321 (2008). https://doi.org/10.1007/s10811-007-9255-7
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DOI: https://doi.org/10.1007/s10811-007-9255-7