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A label-free electrochemical immunosensor based on AuNPs/Zn/Ni-ZIF-8-800@graphene for the detection of wheat gliadin

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Abstract

Wheat gliadin is thought to be the main cause of wheat allergy leading to celiac disease. In this study, we reported an electrochemical immunosensor based on AuNPs/Zn/Ni-ZIF-8-800@graphene for simple, efficient, and sensitive detection of gliadin. A monoclonal antibody against gliadin were prepared by hybridoma technique. Zn/Ni-ZIF-8-800 with high stability was prepared by using zinc–nickel bimetallic organic framework MOFs, and graphene and gold nanoparticles with large surface area and excellent conductivity were introduced. Chitosan was used as a binder to fixed the composite on the surface of glassy carbon electrode. The fabricated materials and the corresponding sensors were comprehensively characterized by X-ray diffractometer, scanning electron microscope, transmission electron microscopy, Fourier transform infrared spectroscopy, Brunauer–Emmett–Teller, and cyclic voltammetry analyses. Under the optimal conditions, the linear range of the electrochemical immunosensor was 0.1–100 μg mL−1, and the detection limit was 0.950 μg mL−1. In addition, the fabricated sensor exhibited high selectivity and long-term stability. The recovery test showed that the prepared sensor was suitable for the detection of gliadin in real food and feed, exhibiting great promising of the developed sensor in practical application.

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Funding

This research was funded by Open Project of Key Laboratory of Animal Immunology of the Ministry of Agriculture (PKLA20170606).

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  1. Ke Zhan and Yongkun Du have contributed equally to this paper.

Authors and Affiliations

  1. College of Food Science and Technology, Henan Agricultural University, Zhengzhou, 450002, Henan, China

    Ke Zhan, Qingqing Liu, Chenxu Cui, Hongtao Ren, Fan Wang, Chao Xu & Na Wang

  2. College of Veterinary Medicine International Joint Research Center for Animal Immunology, Zhengzhou, 450046, Henan, China

    Yongkun Du, Qingqing Liu, Shanshan Li & Na Wang

  3. Longhu Laboratory of Advanced Immunology, Zhengzhou, 450046, China

    Yongkun Du & Na Wang

  4. Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, Henan, China

    Ke Zhan, Qingqing Liu, Chenxu Cui, Guangxu Xing, Shanshan Li, Hongtao Ren, Fan Wang & Na Wang

  5. Key Laboratory of Nutrition and Healthy Food of Zhengzhou, Zhengzhou, 450002, Henan, China

    Ke Zhan, Yongkun Du, Qingqing Liu, Chenxu Cui, Guangxu Xing, Shanshan Li, Hongtao Ren, Fan Wang, Chao Xu & Na Wang

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  1. Ke Zhan
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Contributions

KZ, NW and QL conceived and designed the experiments. KZ, YD, CC and SL performed the experiments. YD, GX, HR and CX contributed data analysis. KZ wrote the manuscript. KZ, NW, FW and CX revised the manuscript content. All authors reviewed the manuscript.

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Correspondence to Chao Xu or Na Wang.

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Zhan, K., Du, Y., Liu, Q. et al. A label-free electrochemical immunosensor based on AuNPs/Zn/Ni-ZIF-8-800@graphene for the detection of wheat gliadin. J Appl Electrochem 54, 669–685 (2024). https://doi.org/10.1007/s10800-023-01978-w

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