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A novel method for electrochemical determination of creatinine in human urine based on its reaction with 2-nitrobenzaldehyde using a glassy carbon electrode

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Abstract

In this work, an electrochemical method for sensitive determination of creatinine has been reported based on its reaction with 2-nitrobenzaldehyde and using differential pulse voltammetry technique. A plausible mechanism of the reaction has been proposed and successful application of this method for creatinine detection in a real sample (human urine) has also been demonstrated. The mechanism is indicative of the formation of multiple electroactive species in the process. The linear range of detection being 1–25 mM, with LOD of 0.50 mM and an excellent R2 value of 0.99, was suitable enough for the detection of creatinine in human urine. Interference studies of urea, uric acid, glucose, ascorbic acid and dopamine were done and found to be within the acceptable limit. All the components except uric acid showed an interference of less than 3.2%, while uric acid showed maximum interference of 8.4%. Its robustness, high selectivity, good sensitivity, and low detection limit have projected it as a promising new tool for a point-of-care testing device.

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Acknowledgements

Mr. Nayab Hussain (DST-INSPIRE fellow, IF180956) would like to thank DST-INSPIRE for funding.

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  1. Department of Chemical Sciences, Tezpur University, Tezpur, Assam, 784028, India

    Nayab Hussain & Panchanan Puzari

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NH conceived the original idea, carried out the experiments, prepared the figures and wrote the first draft of the manuscript. Both NH and PP carried out the analyses and interpretations. PP supervised the work, provided critical feedback and helped to shape the final manuscript.

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Correspondence to Panchanan Puzari.

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Hussain, N., Puzari, P. A novel method for electrochemical determination of creatinine in human urine based on its reaction with 2-nitrobenzaldehyde using a glassy carbon electrode. J Appl Electrochem 54, 175–187 (2024). https://doi.org/10.1007/s10800-023-01938-4

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