Skip to main content
SpringerLink
Log in

A novel and ultrasensitive non-enzymatic electrochemical glucose sensor in real human blood samples based on facile one-step electrochemical synthesis of nickel hydroxides nanoparticles onto a three-dimensional Inconel 625 foam

  • Research Article
  • Published:
Journal of Applied Electrochemistry Aims and scope Submit manuscript

Abstract

In this contribution, a simple and novel non-enzymatic electrochemical sensor for the detection of glucose was successfully prepared by direct in situ growth of nickel hydroxide nanoparticles (Ni(OH)2NPs) onto a three-dimensional Inconel 625foam (IN625F) substrate through a facile electrochemical route, using cyclic voltammetry (CV) method in alkaline medium without addition of nickel salts. Then, surface characterization of modified Ni(OH)2/IN625F electrodes was carried out through advanced technologies, such as scanning electron microscopy (SEM) and X-ray diffraction (XRD). The electrochemical catalytic behavior of the fabricated electrodes was investigated using CV and amperometric methods. The results revealed that the novel modified sensor, Ni(OH)2/IN625F, showed the highest sensitivity of 5685 μAmM−1 cm−2 over a wide linear concentration range from 1 to10 mM, with lowest detection limit (LOD) of 2 μM (S/N = 3), and short response time within < 2 s. Therefore, the proposed non-enzymatic electrochemical sensor demonstrated high selectivity and stability, good reproducibility, and low cost. In addition, analysis of human blood samples was performed. Hence, the constructed glucose sensor, Ni(OH)2/IN625F, with suitable performance could be used as a promising material in real human blood samples.

Graphical abstract

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  1. Li H, Zhang L, Mao Y, Wen C, Zhao P (2019) A simple electrochemical route to access amorphous Co-Ni hydroxide for nonenzymatic glucose sensing. Nanoscale Res Lett 14:135. https://doi.org/10.1186/s11671-019-2966-2

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Ojani R, Raoof JB, Fathi S (2012) Nickel/poly(o-aminophenol) film prepared in presence of sodium dodecyl sulfate: application for electrocatalytic oxidation of carbohydrates. J Chin Chem Soc 59:788–792. https://doi.org/10.1002/jccs.201100463

    Article  CAS  Google Scholar 

  3. Wang Q, Zhang Y, Ye W, Wang C (2016) Ni(OH)2/MoSx nanocomposite electrodeposited on a flexible CNT/PI membrane as an electrochemical glucose sensor: the synergistic effect of Ni(OH)2 and MoSx. J Solid State Electrochem 20:133–142. https://doi.org/10.1007/s10008-015-3002-9

    Article  CAS  Google Scholar 

  4. King H, Aubert RE, Herman WH (1998) Global burden of diabetes, 1995–2025: prevalence, numerical estimates, and projections. Diabetes Care 21:1414–1431. https://doi.org/10.2337/diacare.21.9.1414

    Article  CAS  PubMed  Google Scholar 

  5. Zhe T, Sun X, Liu Y, Wang Q, Li F, Bu T, Jia P, Lu Q, Wang J, Wang L (2019) An integrated anode based on porous Ni/Cu(OH)2 nanospheres for nonenzymatic glucose sensing. Microchem J 151:104197. https://doi.org/10.1016/j.microc.2019.104197

    Article  CAS  Google Scholar 

  6. Rungsawang T, Punrat E, Adkins J, Henry C, Chailapakul O (2016) Development of electrochemical paper-based glucose sensor using cellulose-4-aminophenylboronic acid-modified screen-printed carbon electrode. Electroanalysis 28:462–468. https://doi.org/10.1002/elan.201500406

    Article  CAS  Google Scholar 

  7. Naik KK, Ratha S, Rout CS (2016) Phase and shape dependent non–enzymatic glucose sensing properties of nickel molybdate. Chemistry Select 1:5187–5195. https://doi.org/10.1002/slct.201600795

    Article  CAS  Google Scholar 

  8. Klonoff DC (2012) Overview of fluorescence glucose sensing: a technology with a bright future. J Diabetes Sci Technol 6:1242–1250. https://doi.org/10.1177/193229681200600602

    Article  PubMed  PubMed Central  Google Scholar 

  9. Filip M, Vlassa M, Coman V, Halmagyi A (2016) Simultaneous determination of glucose, fructose, sucrose and sorbitol in the leaf and fruit peel of different apple cultivars by the HPLC–RI optimized method. Food Chem 199:653–659. https://doi.org/10.1016/j.foodchem.2015.12.060

    Article  CAS  PubMed  Google Scholar 

  10. Kremeskotter J, Wilson R (1995) Detection of glucose via electrochemiluminescence in a thin-layer cell with a planar optical waveguide. Meas Sci Technol 6:1325–1328. https://doi.org/10.1088/0957-0233/6/9/012

    Article  Google Scholar 

  11. Morikawa MA, Kimizuka N (2002) New colorimetric detection of glucose by means of electron-accepting indicators: ligand substitution of [Fe(acac)3-n(phen)n]n+ complexes triggered by electron transfer from glucose oxidase. Chem Eur J 8:5580–5584. https://doi.org/10.1002/1521-3765(20021216)8:24%3c5580:AID-CHEM5580%3e3.0.CO;2-V

    Article  CAS  PubMed  Google Scholar 

  12. Li HB, Zhao P (2019) Amorphous Ni-Co-Fe hydroxide nanospheres for the highly sensitive and selective non-enzymatic glucose sensor applications. J Alloys Compd 800:261–271. https://doi.org/10.1016/j.jallcom.2019.05.264

    Article  CAS  Google Scholar 

  13. Liu B, Li Z (2019) Electrochemical treating of a smooth Cu-Ni-Zn surface into layered micro-chips of rice grain-like Cu/Ni(OH)2 nanocomposites as a highly sensitive enzyme-free glucose sensor. J Electroanal Chem 855:113493. https://doi.org/10.1016/j.jelechem.2019.113493

    Article  CAS  Google Scholar 

  14. Wang L, Nie F, Zheng J (2013) Nickel hydroxide and intercalated graphene with ionic liquid nanocomposite modified electrode for sensing of glucose. J Chin Chem Soc 60:1062–1069. https://doi.org/10.1002/jccs.201200587

    Article  CAS  Google Scholar 

  15. Chelaghmia ML, Nacef M, Affoune AM, Pontie M, Derabla T (2018) Facile synthesis of Ni(OH)2 modified disposable pencil graphite electrode and its application for highly sensitive non-enzymatic glucose sensor. Electroanalysis 30:1117–1124. https://doi.org/10.1002/elan.201800002

    Article  CAS  Google Scholar 

  16. Wang L, Lu X, Ye Y, Sun L, Song Y (2013) Nickel-cobalt nanostructures coated reduced graphene oxide nanocomposite electrode for nonenzymatic glucose biosensing. Electrochimi Acta 114:484–493. https://doi.org/10.1016/j.electacta.2013.10.125

    Article  CAS  Google Scholar 

  17. Sim H, Kim JH, Lee SK, Song MJ, Yoon DH, Lim DS, Hong SI (2012) High-sensitivity non-enzymatic glucose biosensor based on Cu(OH)2 nanoflower electrode covered with boron-doped nanocrystalline diamond layer. Thin Solid Films 520:7219–7223. https://doi.org/10.1016/j.tsf.2012.08.011

    Article  CAS  Google Scholar 

  18. Nacef M, Chelaghmia ML, Affoune AM, Pontié M (2019) Electrochemical investigation of glucose on a highly sensitive nickel-copper modified pencil graphite electrode. Electroanalysis 31:113–120. https://doi.org/10.1002/elan.201800622

    Article  CAS  Google Scholar 

  19. Boukharouba C, Nacef M, Chelaghmia ML, Kihal R, Drissi W, Fisli H, Affoune AM, Pontié M (2022) Dendritic Cu(OH)2 nanostructures decorated pencil graphite electrode as a highly sensitive and selective impedimetric non-enzymatic glucose sensor in real human serum blood samples. Monatsh Chemie 153:171–181. https://doi.org/10.1007/s00706-021-02883-8

    Article  CAS  Google Scholar 

  20. Rinaldi AL, Sobral S, Carballo R (2017) Nickel hydroxide nanoparticles on screen printed electrodes as an impedimetric non-enzymatic glucose sensor. Electroanalysis 29:1961–1967. https://doi.org/10.1002/elan.201700187

    Article  CAS  Google Scholar 

  21. Marini S, Ben Mansour N, Hjiri M, Dhahri R, Mir El, Espro LC, Bonavita A, Galvagno S, Neri G, Leonardi SG (2018) Non-enzymatic glucose sensor based on nickel/carbon composite. Electroanalysis 30:727–733. https://doi.org/10.1002/elan.201700687

    Article  CAS  Google Scholar 

  22. Chelaghmia ML, Nacef M, Fisli H, Affoune AM, Pontié M, Makhlouf A, Derabla T, Khelifi O, Aissat F (2020) Electrocatalytic performance of Pt-Ni nanoparticles supported on an activated graphite electrode for ethanol and 2-propanol oxidation. RSC Adv 10:36941–36948. https://doi.org/10.1039/D0RA07331H

    Article  Google Scholar 

  23. Wu X, Li F, Zhao C, Qian X (2018) One-step construction of hierarchical Ni(OH)2/ RGO/Cu2O on Cu foil for ultra-sensitive non-enzymatic glucose and hydrogen peroxide detection. Sens Actuators B Chem 274:163–171. https://doi.org/10.1016/j.snb.2018.07.141

    Article  CAS  Google Scholar 

  24. Yang D, Gao L, Yang JH (2017) Facile synthesis of ultrathin Ni(OH)2-Cu2S hexagonal nanosheets hybrid for oxygen evolution reaction. J Power Sources 359:52–56. https://doi.org/10.1016/j.jpowsour.2017.05.034

    Article  CAS  Google Scholar 

  25. Liu P, Qin K, Wen S, Wang L, He F, Liu E, He C, Li CSJ, Li Q, Ma L, Zhao N (2018) In situ fabrication of Ni(OH)2/Cu2O nanosheets on nanoporous NiCu alloy for high performance supercapacitor. Electrochimi Acta 283:970–978. https://doi.org/10.1016/j.electacta.2018.07.007

    Article  CAS  Google Scholar 

  26. Kung CW, Cheng YH, Ho KC (2014) Single layer of nickel hydroxide nanoparticles covered on a porous Ni foam and its application for highly sensitive non-enzymatic glucose, sensor. Sens Actuators B 204:159–166. https://doi.org/10.1016/j.snb.2014.07.102

    Article  CAS  Google Scholar 

  27. Chelaghmia ML, Nacef M, Affoune AM (2012) Ethanol electrooxidation on activated graphite supported platinum-nickel in alkaline medium. J Appl Electrochem 42:819–826. https://doi.org/10.1007/s10800-012-0440-2

    Article  CAS  Google Scholar 

  28. Mao W, He H, Ye Z, Huang J (2019) Three-dimensional graphene foam integrated with Ni(OH)2 nanosheets as a hierarchical structure for non-enzymatic glucose sensing. J Electroanal Chem 832:275–283. https://doi.org/10.1016/j.jelechem.2018.11.016

    Article  CAS  Google Scholar 

  29. Jia L, Wei X, Lv L, Zhang X, Duan X, Xu Y, Liu K, Wang J (2018) Electrodeposition of hydroxyapatite on nickel foam and further modification with conductive polyaniline for non-enzymatic glucose sensing. Electrochim Acta 280:315–322. https://doi.org/10.1016/j.electacta.2018.05.130

    Article  CAS  Google Scholar 

  30. Zhang Y, Zhao D, Zhu W, Zhang W, Yue Z, Wang J, Wang R, Zhang D, Wang J, Zhang G (2018) Engineering multistage nickel oxide rod-on-sheet nanoarrays on Ni foam: A superior catalytic electrode for ultrahigh-performance electrochemical sensing of glucose. Sens Actuators B Chem 255:416–423. https://doi.org/10.1016/j.snb.2017.08.078

    Article  CAS  Google Scholar 

  31. Ramkumar KD, Abraham WS, Viyash V, Arivazhagan N, Rabel AM (2017) Investigations on the microstructure, tensile strength and high temperature corrosion behaviour of Inconel 625 and Inconel 718 dissimilar joints. J Manuf Process 25:306–322. https://doi.org/10.1016/j.jmapro.2016.12.018

    Article  Google Scholar 

  32. Shakil M, Ahmad M, Tariq NH, Hasan BA, Akhter JI, Ahmed E, Mehmood M, Choudhry MA, Iqbal M (2014) Microstructure and hardness studies of electron beam welded Inconel 625 and stainless steel 304L. Vacuum 110:121–126. https://doi.org/10.1016/j.vacuum.2014.08.016

    Article  CAS  Google Scholar 

  33. Carroll EB, Otis AR, Borgonia JP, Suh JO, Dillon PR, Shapiro AA, Hofmann DC, Liu ZK, Beese AM (2016) Functionally graded material of 304L stainless steel and inconel 625 fabricated by directed energy deposition: characterization and thermodynamic modeling. Acta Mater 108:46–54. https://doi.org/10.1016/j.actamat.2016.02.019

    Article  CAS  Google Scholar 

  34. Babu MS, Kiruba M, Dharuman N, Sundaravignesh S, Sankarapandian S, Prabu V, Berchmans LJ, Sreedhar G (2019) High-temperature oxidation and hot corrosion behavior of Er2Sn2O7+Inconel 625 composite. Ceram Int 45:17620–17629. https://doi.org/10.1016/j.ceramint.2019.05.327

    Article  CAS  Google Scholar 

  35. Urso M, Torrisi G, Boninelli S, Bongiorno C, Priolo F, Mirabella S (2019) Ni(OH)2@Ni core-shell nanochains as low-cost high-rate performance electrode for energy storage applications. Sci Rep 9:7736. https://doi.org/10.1038/s41598-019-44285-1

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  36. Wu MS, Sie YJ, Yang SB (2019) Hollow mesoporous nickel dendrites grown on porous nickel foam for electrochemical oxidation of urea. Electrochim Acta 304:131–137. https://doi.org/10.1016/j.electacta.2019.02.100

    Article  CAS  Google Scholar 

  37. Nacef M, Chelaghmia ML, Khelifi O, Pontié M, Djelaibia M, Guerfa R, Bertagna V, Vautrin-Ul C, Aisset F, Affoune AM (2021) Electrodeposited Ni on pencil graphite electrode for glycerol electrooxidation in alkaline media. Int J Hydrog Energy 46:37670–37678. https://doi.org/10.1016/j.ijhydene.2020.07.104

    Article  CAS  Google Scholar 

  38. Veerasubramani GK, Krishnamoorthy K, Kim SJ (2016) Improved electrochemical performances of binder-free CoMoO4 nanoplate arrays@Ni foam electrode using redox additive electrolyte. J Power Sources 306:378–386. https://doi.org/10.1016/j.jpowsour.2015.12.034

    Article  CAS  Google Scholar 

  39. Ferrari AGM, Foster CW, Kelly PJ, Brownson DAC, Banks CE (2018) Determination of the electrochemical area of screen-printed electrochemical sensing platforms. Biosensors 8:53. https://doi.org/10.3390/bios8020053

    Article  CAS  Google Scholar 

  40. Jafarian M, Forouzandeh F, Danaee I, Gobal F, Mahjani MG (2009) Electrocatalytic oxidation of glucose on Ni and NiCu alloy modified glassy carbon electrode. J Solid State Electrochem 13:1171–1179. https://doi.org/10.1007/s10008-008-0632-1

    Article  CAS  Google Scholar 

  41. Mathew M, Sandhyarani N (2013) A highly sensitive electrochemical glucose sensor structuring with nickel hydroxide and enzyme glucose oxidase. Electrochim Acta 108:274–280. https://doi.org/10.1016/j.electacta.2013.07.010

    Article  CAS  Google Scholar 

  42. Laviron E (1979) General expression of the linear potential sweep voltammogram in the case of diffusionnless electrochemical systems. J Electroanal Chem 101:19–28. https://doi.org/10.1016/S0022-0728(79)80075-3

    Article  CAS  Google Scholar 

  43. Mao H, Cao Z, Guo X, Liu M, Sun D, Sun Z, Ge H, Zhang Y, Song XM (2019) Enhanced electrocatalytic performance for the oxidation of methanol by hierarchical NiS/Ni(OH)2@polypyrrole/graphene oxide nanosheets. Appl Surf Sci 471:355–367. https://doi.org/10.1016/j.apsusc.2018.11.188

    Article  CAS  Google Scholar 

  44. Chelaghmia ML, Fisli H, Nacef M, Brownson DAC, Affoune AM, Satha H, Banks CE (2021) Disposable non-enzymatic electrochemical glucose sensors based upon screen-printed graphite macroelectrodes modified via a facile methodology with Ni, Cu, and Ni/Cu hydroxides are shown to accurately determine glucose in real human serum blood samples. Anal Methods 13:2812–2822. https://doi.org/10.1039/D1AY00056J

    Article  CAS  PubMed  Google Scholar 

  45. Dong M, Hu H, Ding S, Wang C (2021) High-performance non-enzymatic glucose-sensing electrode fabricated by a-nickel hydroxide-reduced graphene oxide nanocomposite on nickel foam substrate. J Mater Sci Mater Electron 32:19327–19338. https://doi.org/10.1007/s10854-021-06451-y

    Article  CAS  Google Scholar 

  46. Wang L, Xie Y, Wei C, Lu X, Li X, Song Y (2015) Hierarchical NiO superstructures/foam Ni electrode derived from Ni metal-organic framework flakes on foam Ni for glucose sensing. Electrochim Acta 174:846–852. https://doi.org/10.1016/j.electacta.2015.06.086

    Article  CAS  Google Scholar 

  47. Zhaoa Y, Gub G, Youa S, Jic R, Suota H, Zhaota C, Liu F (2015) Preparation of Ni(OH)2 nanosheets on Ni foam via direct precipitation method for highly sensitive non-enzymatic glucose sensor. RSC Adv 5:53665–53670. https://doi.org/10.1039/C5RA06664F

    Article  CAS  Google Scholar 

  48. Xiao Q, Wang X, Huang S (2017) Facile synthesis of Ni(OH)2 nanowires on nickel foam via one step low-temperature hydrothermal route for non-enzymatic glucose sensor. Mater Lett 198:19–22. https://doi.org/10.1016/j.matlet.2017.03.172

    Article  CAS  Google Scholar 

  49. Joa HJ, Shita A, Jhonc HS, Park SY (2020) Highly sensitive non-enzymatic wireless glucose sensor based on Ni–Co oxide nanoneedle-anchored polymer dots. J Ind Eng Chem 89:485–493. https://doi.org/10.1016/j.jiec.2020.06.028

    Article  CAS  Google Scholar 

  50. Lu W, Qin X, Asiri AM, Al-Youbi AO, Sun X (2013) Ni foam: a novel three-dimensional porous sensing platform for sensitive and selective nonenzymatic glucose detection. Analyst 138:417–420. https://doi.org/10.1039/C2AN36138H

    Article  CAS  PubMed  Google Scholar 

  51. Huo H, Zhao Y, Xu C (2014) 3D Ni3S2 nanosheet arrays supported on Ni foam for high-performance supercapacitor and nonenzymatic glucose detection. J Mater Chem A 2:15111–15117. https://doi.org/10.1039/C4TA02857K

    Article  CAS  Google Scholar 

  52. Kumar S, Fu YP (2021) PANI/g-C3N4 composite over ZnCo2O4/Ni-foam, a bi-functional electrode as a supercapacitor and electrochemical glucose sensor. Sustain Energy Fuels 5:3987–4001. https://doi.org/10.1039/D1SE00491C

    Article  CAS  Google Scholar 

  53. Iwu KO, Lombardo A, Sanz R, Scire S, Mirabella S (2016) Facile synthesis of Ni nanofoam for flexible and low-cost non-enzymatic glucose sensing. Sens Actuators B: Chem 224:764–771. https://doi.org/10.1016/j.snb.2015.10.109

    Article  CAS  Google Scholar 

  54. Wang X, Wang M, Feng S, He D, Jiang P (2020) Controlled synthesis of flower-like cobalt phosphate microsheet arrays supported on Ni foam as a highly efficient 3D integrated anode for non-enzymatic glucose sensing. Inorg Chem Front 7:108–116. https://doi.org/10.1039/C9QI00948E

    Article  CAS  Google Scholar 

  55. Xia K, Yang C, Chenb Y, Tianb L, Sub Y, Wang J, Li L (2017) In situ fabrication of Ni(OH)2 flakes on Ni foam through electrochemical corrosion as high sensitive and stable binder-free electrode for glucose sensing. Sens Actuators B 240:979–987. https://doi.org/10.1016/j.snb.2016.09.077

    Article  CAS  Google Scholar 

  56. Guo Q, Zeng W, Li Y (2019) Highly sensitive non-enzymatic glucose sensor based on porous NiCo2O4 nanowires grown on nickel foam. Mater Lett 256:126603. https://doi.org/10.1016/j.matlet.2019.126603

    Article  CAS  Google Scholar 

  57. Chandrasekaran NI, Matheswaran M (2019) A sensitive and selective non-enzymatic glucose sensor with hollow Ni-AlMn layered triple hydroxide nanocomposites modified Ni foam. Sens Actuators B: Chem 288:188–194. https://doi.org/10.1016/j.snb.2019.02.102

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The financial support within the General Direction of Scientific Research and Technology Development of the Algerian Ministry of Higher Education and Scientific Research is greatly acknowledged.

Author information

Authors and Affiliations

  1. Laboratoire d’Analyses Industrielles et Génie Des Matériaux, Université 8 Mai 1945 Guelma 24000, BP 401, Guelma, Algeria

    Rafiaa Kihal, Mohamed Lyamine Chelaghmia, Widad Drissi, Chahira Boukharouba, Sara Abdi, Mouna Nacef & Abed Mohamed Affoune

  2. Université Abbes Laghrour Khenchela, BP 1252 Route de Batna, Khenchela 40004, Algeria

    Rafiaa Kihal

  3. Laboratoire de Chimie Appliquée, Université 8 Mai 1945 Guelma 24000, BP 401, Guelma, Algeria

    Hassina Fisli

  4. Univ. Angers, Group Analysis and Processes, 49045, Angers 01, France

    Maxime Pontié

Authors
  1. Rafiaa Kihal
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hassina Fisli
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mohamed Lyamine Chelaghmia
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Widad Drissi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Chahira Boukharouba
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Sara Abdi
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Mouna Nacef
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Abed Mohamed Affoune
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Maxime Pontié
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mohamed Lyamine Chelaghmia.

Ethics declarations

Conflict of interest

The authors declare no competing financial interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kihal, R., Fisli, H., Chelaghmia, M.L. et al. A novel and ultrasensitive non-enzymatic electrochemical glucose sensor in real human blood samples based on facile one-step electrochemical synthesis of nickel hydroxides nanoparticles onto a three-dimensional Inconel 625 foam. J Appl Electrochem 53, 315–329 (2023). https://doi.org/10.1007/s10800-022-01757-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10800-022-01757-z

Keywords

Search

Navigation