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Electrochemical self-signal identification of Kirsten rat sarcoma virus oncogene based on riboflavin 5′-(trihydrogen diphosphate) functionalized WS2 nanosheets

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Abstract

A novel electrochemical bioidentification system for determination of the Kirsten rat sarcoma virus oncogene (KRAS) gene derived from lung cancer was developed based on riboflavin 5′-(trihydrogen diphosphate) (RHP) functionalized WS2 nanosheets prepared by synchronous ultrasonication. RHP presented excellent performance for scattering the WS2 nanosheets into aqueous phase. The acquired WS2-RHP nanocomposite provided favorable interface for DNA sensing by means of electrochemical self-redox signal variation. The immobilization of the probe single-stranded DNA (ssDNA) on the nanocomposite through the non-covalent π–π interaction between the nucleic acid bases and conjugated nanostructure could bring about the decrease of the self-signal. The self-redox signal would regenerate owing to the release of the obtained double-stranded DNA (dsDNA) from the nanocomposite due to the burying of the base in dsDNA after the nucleic acid hybridization. The determination linear range of the complementary target DNA was achieved from 1.0 × 10−16 to 1.0 × 10−8 mol L−1 accompanied with a detection limit of 2.7 × 10−17 mol L−1. The constructed gene sensing system exhibited splendid specificity and magnificent long-term stableness at the same time. The proposed detection method was deserved to spread and employed to develop diverse sensing systems for biological species and molecules.

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Acknowledgements

This work was financed by the Natural Science Foundation of Shandong Province (No. ZR2021MB131 and ZR2021MB105).

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  1. School of Chemistry and Chemical Engineering, Linyi University, Linyi, 276000, China

    Jimin Yang, Xiaowei Hu & Wei Zhang

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  1. Jimin Yang
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  2. Xiaowei Hu
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  3. Wei Zhang
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Correspondence to Wei Zhang.

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Yang, J., Hu, X. & Zhang, W. Electrochemical self-signal identification of Kirsten rat sarcoma virus oncogene based on riboflavin 5′-(trihydrogen diphosphate) functionalized WS2 nanosheets. J Appl Electrochem 53, 19–27 (2023). https://doi.org/10.1007/s10800-022-01739-1

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  • DOI: https://doi.org/10.1007/s10800-022-01739-1

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