Abstract
This study evaluated the composition and the antinociceptive and anti-inflammatory activity of the crude extracts and two isolated compounds, anamarine (ANA) and 10-epi-olguine (eOL), obtained from the leaves of Cantinoa stricta (Lamiaceae). Crude ethanolic extract (EEt) and dichloromethane extract (DCM), selected based on NMR data, were submitted to pharmacological tests in male Swiss mice. The oral administration of EEt and DCM significantly reduced the second phase of formalin-induced nociception (60%), lipopolysaccharide (LPS)-induced mechanical hyperalgesia (90%), and carrageenan (Cg)-induced edema (25%). ANA and eOL, the major compounds in EEt and DCM extracts, administered orally or locally (in the paw), also reduced the LPS-induced mechanical hyperalgesia (Oral ID50 1.9 and 3.9 mg/kg; Local ID50 93.4 and 677.3 ng, respectively) without changing the thermal acute nociception or the motor performance of the animals. Local administration of ANA and eOL also reduced Cg-induced edema (40 and 23%, respectively). These isolated compounds did not change the mechanical hyperalgesia induced by tumor necrosis factor-α, interleukin-1β, prostaglandin E2, dibutyryl cyclic AMP, or forskolin but reversed the hyperalgesia induced by dopamine, epinephrine, and phorbol 12-myristate 13-acetate. The hyperalgesia induced by epinephrine was reversed in male but not in female mice, in which this response is not dependent on protein kinase C (PKC). These results suggest that C. stricta extracts possess antinociceptive and anti-inflammatory activity which is related to the presence of ANA and eOL. Differently from the known analgesics, these substances seem to exert their action mainly interfering with the sympathetic component of pain, possibly with PKC.
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The datasets generated during the current study are available from the corresponding author on reasonable request.
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Acknowledgements
FLB, DRR and CSO are grateful to Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—CAPES (Finance Code 001). CAE thanks Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for scholarship. MEAS (process 427859/2018-2) and ARZ (process 309829/2020-8) also thanks CNPq for scholarship. Thanks also are due to Elide P. Santos for collection and identification of the pant.
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This work was supported by grant #457938 from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).
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Barbosa, F.L., Ehrenfried, C.A., Radulski, D.R. et al. Antinociceptive and anti-inflammatory activity of extracts and α-pyrones isolated from Cantinoa stricta. Inflammopharmacol 32, 1263–1275 (2024). https://doi.org/10.1007/s10787-024-01444-9
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DOI: https://doi.org/10.1007/s10787-024-01444-9