Abstract
Ulcerative colitis is a form of inflammatory bowel disease that is characterized by acute and chronic inflammation. The aim of this work was to evaluate the efficacy of hydroethanolic extract of Maesa lanceolata leaves on acetic acid-induced colitis in rats. Colitis was induced by rectal administration of 1 mL of acetic acid (4%) in 25 male rats except the normal control group which received distilled water after 18 h of fasting followed by Ketamine (50 mg/kg)/Valium (10 mg/kg) anesthesia. Five hours later, the normal control and the negative control received distilled water, the positive control received prednisolone (5 mg/kg) and the three test groups received extract at 100, 200 and 400 mg/kg bw for eight days. During treatment, rectal temperature, the number and quality of the stools, and changes in body weight were assessed. At the end of the treatment, the animals were sacrificed, blood, colon, liver and spleen were collected for evaluation of hematological, inflammatory, antioxidant and histological parameters. Rectal temperature and the number of diarrheal, mucus and bloody stools were significantly reduced (P < 0.01) during treatment in the test and positive control groups with an increase in body weight change. The extract significantly (P < 0.01) reduced myeloperoxidase, TNF-α, interleukin 6, NO and MDA levels and significantly (P < 0.01) increased SOD levels, of GSH and catalase activity in the colon and blood. This extract also increased (P < 0.01) levels of red blood cells, hemoglobin, hematocrit, total white blood cells and blood platelets, prevented leukocyte infiltration in the liver and colon.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Data availability
The data used to support the findings of this study are included within the article.
References
Amaral EP, Vinhaes CL, Oliveira-de-Souza D, Nogueira B, Akrami KM, Andrade BB (2020) The interplay between systemic inflammation, oxidative stress, and tissue remodeling in tuberculosis. Antioxid Redox Signal 00:1–15. https://doi.org/10.1089/ars.2020.8124
Andrade AWL, Guerra GCB, De Souza Araújo DF, De Araújo Júnior RF, De Araújo AA, De Carvalho TG, Fernandes JM, Diez-Echave P, Hidalgo-García L, Rodriguez-Cabezas ME, Gálvez J, Zucolotto SM (2020) Anti-inflammatory and chemopreventive effects of Bryophyllum pinnatum (lamarck) leaf extract in experimental colitis models in rodents. Front Pharmacol 11:18. https://doi.org/10.3389/fphar.2020.00998
Baumgart DC, Sandborn WJ (2012) Crohn’s disease. Lancet 380:1590–1605. https://doi.org/10.1016/S0140-6736(12)60026-9
Bouaziz M, Grayer RJ, Simmonds MSJ, Damak M, Sayadi S (2005) Identification and antioxidant potential of flavonoids and low molecular weight phenols in olive cultivar Chemlali growing in Tunisia. J Agric Food Chem 53:236–241. https://doi.org/10.1021/jf048859d
Cagin YF, Parlakpinar H, Vardi N, Polat A, Atayan Y, Erdogan MA, Tanbek K (2016) Effects of dexpanthenol on acetic acid-induced colitis in rats. Exp Ther Med 12:2958–2964. https://doi.org/10.3892/etm.2016.3728
Cosnes J, Gower-Rousseau C, Seksik P, Cortot A (2011) Epidemiology and natural history of inflammatory bowel diseases. Gastroenterology 140:1785-1794.e4. https://doi.org/10.1053/j.gastro.2011.01.055
Dan A, Boutros M, Nedja H et al (2017) Cost of ulcerative colitis in Quebec, Canada: a retrospective cohort study. Inflamm Bowel Dis 23:1262–1271. https://doi.org/10.1097/MIB.0000000000001147
Dileep R, Usharani M, Gopu B, Reddy AG, Kumar MV, Satish Kumar CSV, Madhuri D (2016) Ameliorative effect of ginger extract in acetic acid induced ulcerative colitis in rats. Asian J Anim Vet Adv 11:130–136. https://doi.org/10.3923/ajava.2016.130.136
Diouf ML, Dia D, Thioubou A, Bassène ML, Mbengue M (2010) Prévalence de la rectocolite hémorragique dans le centre d’endoscopie digestive de l’hôpital Aristide-Le-Dantec de Dakar. J Afr D’hepato-Gastroenterol 4:97–102. https://doi.org/10.1007/s12157-010-0158-x
Duclos B (2017) Physiopathologie des maladies inflammatoires chroniques intestinales. JMV-J Méd Vasc 42:91–92. https://doi.org/10.1016/j.jdmv.2017.01.090
Ellman GL (1959) Tissue sulfhydryl groups. Arch Biochem Biophys 82:70–77. https://doi.org/10.1016/0003-9861(59)90090-6
Essafi-Benkhadir K, Refai A, Riahi I, Fattouch S, Karoui H, Essafi M (2012) Quince (Cydonia oblonga Miller) peel polyphenols modulate LPS-induced inflammation in human THP-1-derived macrophages through NF-κB, p38MAPK and Akt inhibition. Biochem Biophys Res Commun 418:180–185. https://doi.org/10.1016/j.bbrc.2012.01.003
Faubion WA, Loftus EV, Harmsen WS, Zinsmeister AR, Sandborn WJ (2001) The natural history of corticosteroid therapy for inflammatory bowel disease: a population-based study. Gastroenterology 121:255–260. https://doi.org/10.1053/gast.2001.26279
Fermor B, Haribabu B, Brice Weinberg J, Pisetsky DS, Guilak F (2001) Mechanical stress and nitric oxide influence leukotriene production in cartilage. Biochem Biophys Res Commun 285:806–810. https://doi.org/10.1006/bbrc.2001.5237
Fokam Tagne MA, Noubissi PA, Gaffo EF, Fankem GO, Ngakou Mukam J, Kamgang R, Essame Oyono J-L (2021) Effects of aqueous extract of Anogeissus leiocarpus (DC) guill. Et Perr. (Combretaceae) leaves on acetic acid-induced ulcerative colitis in rats. Adv Tradit Med. https://doi.org/10.1007/s13596-021-00572-9
Foubert K, Breynaert A, Theunis M, Van Den Bossche R, De Meyer GRY, Van Daele A, Faizal A, Goossens A, Geelen D, Conway EM, Vlietinck A, Pieters L, Apers S (2012) Evaluation of the anti-angiogenic activity of saponins from Maesa lanceolata by different assays. Nat Prod Commun 7:1149–1154. https://doi.org/10.1177/1934578x1200700910
Hanauer SB (2006) Inflammatory bowel disease: epidemiology, pathogenesis, and therapeutic opportunities. Inflamm Bowel Dis 12:S3–S9
Hur H, Sung H, Bessell MS (2012) Distance and the initial mass function of young open clusters in the η Carina Nebula: Tr14 and Tr16. Astron J 143:10. https://doi.org/10.1088/0004-6256/143/2/41
Jagtap AG, Shirke SS, Phadke AS (2004) Effect of polyherbal formulation on experimental models of inflammatory bowel diseases. J Ethnopharmacol 90:195–204. https://doi.org/10.1016/j.jep.2003.09.042
Jess T, Gamborg M, Matzen P (2005) Increased risk of intestinal cancer in Crohn’s disease: a meta-analysis of population-based cohort studies. Am J Gastroenterol 100:2724–2729. https://doi.org/10.1111/j.1572-0241.2005.00287.x
Lenoir L, Joubert-zakeyh J, Texier O, Lamaison J-L, Vasson M, Felgines C (2012) Aloysia triphylla infusion protects rats against dextran sulfate sodium-induced colonic damage. J Sci Food Agric 92:1570–1572. https://doi.org/10.1002/jsfa.5544
Liang XT, Fang WS (2006) Medicinal chemistry of bioactive natural products. Wiley
Marck V (2010) Anatomie et cytologie pathologiques. In: Marck V (ed) Manuel de techniques d’anatomo-cytopathologie, Masson SAS. Elsevier, Paris, pp 35–132
Mastorakos G, Ilias I (2003) Maternal and fetal hypothalamic-pituitary-adrenal axes during pregnancy and postpartum. Ann NY Acad Sci 997:136–149. https://doi.org/10.1196/annals.1290.016
Milton AS (1989) Thermoregulatory actions of eicosanoids in the central nervous system with particular regard to the pathogenesis of fever. Ann NY Acad Sci 559:392–410. https://doi.org/10.1111/j.1749-6632.1989.tb22625.x
Misra HP, Fridovich I (1972) The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem 247:3170–3175
Muthu C, Ayyanar M, Raja N, Ignacimuthu S (2006) Medicinal plants used by traditional healers in Kancheepuram district of Tamil Nadu. India J Ethnobiol Ethnomed 2:43. https://doi.org/10.1186/1746-4269-2-43
Nikkhah-Bodaghi M, Darabi Z, Agah S, Hekmatdoost A (2019) The effects of Nigella sativa on quality of life, disease activity index, and some of inflammatory and oxidative stress factors in patients with ulcerative colitis. Phyther Res 33:1027–1032. https://doi.org/10.1002/ptr.6296
Otari KV, Gaikwad PS, Shete RV, Upasani CD (2012) Protective effect of aqueous extract of Spinacia oleracea leaves in experimental paradigms of inflammatory bowel disease. Inflammopharmacology 20:277–287. https://doi.org/10.1007/s10787-011-0114-4
Palla AR, Kennedy D, Mosharraf H, Doll D (2016) Autoimmune hemolytic anemia as a complication of nivolumab therapy. Case Rep Oncol 9:691–697. https://doi.org/10.1002/hon.2338
Pradeep PM, Sreerama YN (2015) Impact of processing on the phenolic profiles of small millets: evaluation of their antioxidant and enzyme inhibitory properties associated with hyperglycemia. Food Chem 169:455–463. https://doi.org/10.1016/j.foodchem.2014.08.010
Rufo PA, Bousvaros A (2006) Current therapy of inflammatory bowel disease in children. Pediatr Drugs 8:279–302. https://doi.org/10.2165/00148581-200608050-00002
Siegel S (2011) The four-loko effect. Perspect Psychol Sci 6:357–362. https://doi.org/10.1177/1745691611409243
Sindambiwe JB, Calomme M, Geerts S, Pieters L, Vlietinck AJ, Vanden Berghe DA (1998) Evaluation of biological activities of triterpenoid saponins from Maesa lanceolata. J Nat Prod 61:585–590. https://doi.org/10.1021/np9705165
Sinha AK (1972) Colorimetric assay of catalase. Anal Biochem 47:389–394. https://doi.org/10.1016/0003-2697(72)90132-7
Smaga I, Niedzielska E, Gawlik M, Moniczewski A, Krzek J, Przegaliński E, Pera J, Filip M (2015) Oxidative stress as an etiological factor and a potential treatment target of psychiatric disorders. Part 2. Depression, anxiety, schizophrenia and autism. Pharmacol Rep 67:569–580. https://doi.org/10.1016/j.pharep.2014.12.015
Smith J, van den Broek F, Jc M, Hackbarth H, Ruksenas O, Zeller W (2007) FELASA Working Grp, and “principles and practice in ethical review of animal experiments across europe: summary of the report of a felasa working group on ethical evaluation of animal experiments.” Lab Anim 41:143–160
Soro TY, Traoré F, Datte JY, Nene-Bi AS (2009) Activité antipyrétique de l’extrait aqueux de Ximenia americana. Phytothérapierapie 7:297–303. https://doi.org/10.1007/s10298-009-0507-3
Tanideh N, Bahrani M, Khoshnood-Mansoorkhani MJ, Mehrabani D, Firoozi D, Koohi-Hosseinabadi O, Iraji A (2016) Evaluating the effect of melillotus officinalis l aqueous extracts on healing of acetic acid-induced ulcerative colitis in male rats. Ann Color Res 4:e42856
Timothy C, Lizzy M, Richard K, Angela M, Christine B (2018) Antimicrobial activity and safety of Maesa lanceolata for the treatment and Management of selected bacterial pathogens. J Adv Microbiol 8:1–8. https://doi.org/10.9734/jamb/2018/39450
Wilbur KM, Bernheim F, Shapiro OW (1949) The thiobarbituric acid reagent as a test for the oxidation of unsaturated fatty acids by various agents. Arch Biochem 24:305–313
Young S, Aman S, Worku D, Akalu D, Young T, Lee H, Lim Y, Yong Y (2014) Identification of an anticancer compound contained in seeds of Maesa lanceolata, a medicinal plant in Ethiopia. J Korean Soc Appl Biol Chem 57:519–522. https://doi.org/10.1007/s13765-014-4177-y
Acknowledgements
The authors thank the Faculty of Science, University of Ngaoundere, Cameroon, for providing the necessary support for this study.
Funding
This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.
Author information
Authors and Affiliations
Contributions
Conceptualization: RK, MAFT, SSD. Formal analysis: MAFT, BK. Investigation: MAFT, PAN, AT, AGM. Methodology: RK, MAFT, AT, AGM, BK, JNM. Project administration: RK, MAFT, SSD. Resources: RK, MAFT. Supervision: RK, SSD. Validation: RK, SSD. Writing—original draft preparation: MAFT. Writing—review & editing: MAFT, PAN, BK, JNM, SSD, RK.
Corresponding author
Ethics declarations
Conflict of interest
The authors of this work declare no conflict of interest.
Ethics approval
The research was approved by the institutional committee of the Cameroonian Ministry of Scientific Research and Innovation according to the guidelines of the European Union on Animal Care. The ethical reference number of our article is “CEE Council 86/60”.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Fokam Tagne, M.A., Tchoffo, A., Noubissi, P.A. et al. Effects of hydro-ethanolic extract of leaves of Maesa lanceolata (Mursinaceae) on acetic acid-induced ulcerative colitis in rats. Inflammopharmacol 29, 1211–1223 (2021). https://doi.org/10.1007/s10787-021-00825-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10787-021-00825-8