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Curcumin ameliorates palmitate-induced inflammation in skeletal muscle cells by regulating JNK/NF-kB pathway and ROS production

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Abstract

Curcumin, a natural polyphenol compound, has the beneficial effects on several diseases such as metabolic syndrome, cancer, and diabetes. The anti-inflammatory property of curcumin has been demonstrated in different cells; however, its role in prevention of palmitate-induced inflammation in skeletal muscle C2C12 cells is not known. In this study, we examined the effect of curcumin on the inflammatory responses stimulated by palmitate in C2C2 cells. The results showed that palmitate upregulated the mRNA expression and protein release of IL-6 and TNF-α cytokines in C2C12 cells, while pretreatment with curcumin was able to attenuate the effect of palmitate on inflammatory cytokines. The anti-inflammatory effect of curcumin was associated with the repression of phosphorylation of IKKα-IKKβ, and JNK. Palmitate also caused an increase in reactive oxygen species (ROS) level that curcumin abrogated it. Collectively, these findings suggest that curcumin may represent a promising therapy for prevention of inflammation in skeletal muscle cells.

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Abbreviations

FFA:

Free fatty acids

TNF-α:

Tumor necrosis factor alpha

IL-6:

Interleukin 6

CRP:

C-reactive protein

IL-1β:

Interleukin 1β

LPS:

Lipopolysaccharide

NF-κB:

Nuclear factor kappa-light-chain-enhancer of activated B cells

IKK:

IκB kinase

MAPK:

Mitogen-activated protein kinase

JNK:

c-Jun N-terminal kinase

ERK:

Extracellular signal-regulated kinase

ROS:

Reactive oxygen species

T2D:

Type 2 diabetes

IκB:

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor

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Acknowledgements

This work was financially supported by a Grant (96-04-30-36707) from the Deputy of Research, Tehran University of Medical Sciences.

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Authors and Affiliations

  1. Department of Biochemistry, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Islamic Republic of Iran

    Asie Sadeghi

  2. Department of Clinical Biochemistry, Faculty of Medicine Sciences, Tarbiat Modares University, Tehran, Islamic Republic of Iran

    Atefeh Rostamirad

  3. Department of Clinical Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran

    Shadisadat Seyyedebrahimi & Reza Meshkani

  4. Department of Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran

    Reza Meshkani

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  1. Asie Sadeghi
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  2. Atefeh Rostamirad
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  4. Reza Meshkani
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Correspondence to Reza Meshkani.

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Sadeghi, A., Rostamirad, A., Seyyedebrahimi, S. et al. Curcumin ameliorates palmitate-induced inflammation in skeletal muscle cells by regulating JNK/NF-kB pathway and ROS production. Inflammopharmacol 26, 1265–1272 (2018). https://doi.org/10.1007/s10787-018-0466-0

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