, Volume 26, Issue 5, pp 1265–1272 | Cite as

Curcumin ameliorates palmitate-induced inflammation in skeletal muscle cells by regulating JNK/NF-kB pathway and ROS production

  • Asie Sadeghi
  • Atefeh Rostamirad
  • Shadisadat Seyyedebrahimi
  • Reza Meshkani
Original Article


Curcumin, a natural polyphenol compound, has the beneficial effects on several diseases such as metabolic syndrome, cancer, and diabetes. The anti-inflammatory property of curcumin has been demonstrated in different cells; however, its role in prevention of palmitate-induced inflammation in skeletal muscle C2C12 cells is not known. In this study, we examined the effect of curcumin on the inflammatory responses stimulated by palmitate in C2C2 cells. The results showed that palmitate upregulated the mRNA expression and protein release of IL-6 and TNF-α cytokines in C2C12 cells, while pretreatment with curcumin was able to attenuate the effect of palmitate on inflammatory cytokines. The anti-inflammatory effect of curcumin was associated with the repression of phosphorylation of IKKα-IKKβ, and JNK. Palmitate also caused an increase in reactive oxygen species (ROS) level that curcumin abrogated it. Collectively, these findings suggest that curcumin may represent a promising therapy for prevention of inflammation in skeletal muscle cells.


Curcumin Inflammation Skeletal muscle cells NF-kB JNK TNF-α IL-6 Reactive oxygen species 



Free fatty acids


Tumor necrosis factor alpha


Interleukin 6


C-reactive protein


Interleukin 1β




Nuclear factor kappa-light-chain-enhancer of activated B cells


IκB kinase


Mitogen-activated protein kinase


c-Jun N-terminal kinase


Extracellular signal-regulated kinase


Reactive oxygen species


Type 2 diabetes


Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor



This work was financially supported by a Grant (96-04-30-36707) from the Deputy of Research, Tehran University of Medical Sciences.

Compliance with ethical standards

Conflict of interest

The authors have nothing to declare.


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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Biochemistry, Afzalipour School of MedicineKerman University of Medical SciencesKermanIslamic Republic of Iran
  2. 2.Department of Clinical Biochemistry, Faculty of Medicine SciencesTarbiat Modares UniversityTehranIslamic Republic of Iran
  3. 3.Department of Clinical Biochemistry, Faculty of MedicineTehran University of Medical SciencesTehranIslamic Republic of Iran
  4. 4.Department of Biochemistry, Faculty of MedicineTehran University of Medical SciencesTehranIslamic Republic of Iran

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