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Piperine Augments the Protective Effect of Curcumin Against Lipopolysaccharide-Induced Neurobehavioral and Neurochemical Deficits in Mice

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Abstract

The aim of the present study was to investigate the protective effects of curcumin alone and in combination with piperine against lipopolysaccharide (LPS)-induced neurobehavioral and neurochemical deficits in the mice hippocampus. Mice were treated with curcumin (100, 200, and 400 mg/kg, p.o.) and piperine (20 mg/kg, p.o.) for 7 days followed by LPS (0.83 mg/kg, i.p.) administration. Animals exhibited anxiety and depressive-like phenotype after 3 and 24 h of LPS exposure, respectively. LPS administration increased the oxido-nitrosative stress as evident by elevated levels of malondialdehyde, nitrite, and depletion of glutathione level in the hippocampus. Furthermore, we found raised level of pro-inflammatory cytokines (IL-1β and TNF-α) in the hippocampus of LPS-treated mice. Pretreatment with curcumin alleviated LPS-induced neurobehavioral and neurochemical deficits. Furthermore, co-administration of curcumin with piperine significantly potentiated the neuroprotective effect of curcumin. These results demonstrate that piperine enhanced the neuroprotective effect of curcumin against LPS-induced neurobehavioral and neurochemical deficits.

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Acknowledgements

We would like to thank the Department of Pharmaceuticals, Ministry of Chemicals and Fertilizers, Government of India, for financial support. The authors are immensely thankful to the Institutional Level Biotech hub, NIPER Guwahati for providing technical support.

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Authors and Affiliations

  1. Department of Pharmacology and Toxicology, Laboratory of Neuroscience, National Institute of Pharmaceutical Education and Research, Guwahati, Assam, 781032, India

    Ashok Jangra, Mohit Kwatra, Tavleen Singh, Rajat Pant, Pawan Kushwah & Yogita Sharma

  2. Department of Biotechnology, University Institute of Engineering & Technology (UIET), Maharishi Dayanand University, Rohtak, Haryana, India

    Babita Saroha

  3. National Brain Research Centre, Manesar, Gurgaon, Haryana, India

    Ashok Kumar Datusalia

  4. Department of Pharmacology, Gauhati Medical College, Guwahati, Assam, India

    Babul Kumar Bezbaruah

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  1. Ashok Jangra
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Jangra, A., Kwatra, M., Singh, T. et al. Piperine Augments the Protective Effect of Curcumin Against Lipopolysaccharide-Induced Neurobehavioral and Neurochemical Deficits in Mice. Inflammation 39, 1025–1038 (2016). https://doi.org/10.1007/s10753-016-0332-4

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