Abstract
The anti-inflammatory activity of Canavalia seed lectins (Canavalia gladiata [CGL], Canavalia maritima [ConM] and Canavalia brasiliensis [ConBr]) was evaluated by intravenous administration in rats. In non-sensitized rats, cellular edema elicited by carrageenan was reduced (45–51 %) by ConM and (44–59 %) by CGL. Osmotic edema elicited by dextran was reduced by ConM and CGL in 27 % and 29 %. ConM and CGL reduced the edema elicited by l-arginine in 53 % and that of prostaglandin E2 in 48 % and 36 %. Leukocyte migration elicited by carrageenan was reduced in 49 % by ConM and in 55 % by CGL (attenuated in 4× by glucose) and peritoneal TNF-α content in 82 %. In rats sensitized, ConM inhibited the paw edema and leukocyte migration elicited by ovalbumin in 34 % and 70 %. ConM and CGL are anti-inflammatory, mainly in cellular events mediated by prostaglandin E2, nitric oxide and TNF-α in non-sensitized rats. However, only ConM is anti-inflammatory in sensitized rats. CGL effect involves the lectin domain.
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Acknowledgments
The authors are thankful to the financial support of Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico (FUNCAP) and Institutos Nacionais de Ciência e Tecnologia (INCT): Instituto de NanoBioEstruturas & Simulação NanoBioMolecular [NANO(BIO)SIMES]. Cavada BS and Assreuy AMS are senior investigators of CNPq/Brazil. The authors declare that there are no conflicts of interest.
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Pinto, N.V., Cavada, B.S., Brito, L.F. et al. Effects of Canavalia lectins on Acute Inflammation in Sensitized and Non-sensitized Rats. Inflammation 36, 713–722 (2013). https://doi.org/10.1007/s10753-013-9596-0
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DOI: https://doi.org/10.1007/s10753-013-9596-0