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The anti-inflamatory effect of Andira anthelmia lectin in rats involves inhibition of the prostanoid pathway, TNF-α and lectin domain

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Abstract

Objective

To investigate the effect and mechanisms of Andira anthelmia lectin in rat models of acute inflammation.

Material

AAL anti-inflammatory activity was evaluated in Wistar rat models of paw edema and peritonitis.

Methods

AAL (0.01–1 mg/kg i.v.) was injected 30 min before stimulation with carrageenan and with initial and late phase inflammatory mediators into the animals paw or peritoneum for evaluation of cell migration (optical and intravital microscopy), paw edema (plethysmometry and histopathology); hyperalgesia (analgesimetry).

Results

AAL inhibited leukocyte migration induced by carrageenan, mainly neutrophils to the peritoneal fluid, decreasing leukocyte adhesion. In the peritoneal fluid, AAL reduced the gene expression of TNF-α and cyclooxygenase, as well the levels of PGE2. AAL inhibited the paw edema induced by carrageenan, serotonin, histamine, TNF-α, PLA2 and PGE2, but not by L-arginine. In this model, AAL also inhibited mechanical hypernociception induced by TNF-α, PGE2, db-cAMP and capsaicin, and the activity of myeloperoxidase in the paw tissues.

Conclusion

AAL presents anti-inflammatory effect in acute models of rat inflammation involving the participation of prostaglandins, TNF-α and lectin domain.

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Acknowledgements

The authors thank Conselho Nacional de Desenvolvimento Científico e Tecnológico-CNPq. Cavada BS, Nascimento KS and Assreuy AM are senior investigators of CNPq.

Funding

Conselho Nacional de Desenvolvimento Científico e Tecnológico,308433/2017-3,Ana Maria Sampaio Assreuy

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Authors and Affiliations

  1. Laboratório de Fisio-Farmacologia da Inflamação (LAFFIN), Instituto Superior de Ciências Biomédicas, Universidade Estadual do Ceará, Av. Dr. Silas Munguba 1700, Fortaleza, CE, CEP 60740-000, Brazil

    Francisco Lucas Faustino do Nascimento, Alana de Freitas Pires, Diego Freitas de Araujo & Ana Maria Sampaio Assreuy

  2. Centro de Ciências da Saúde, Unidade Via Corpvs, Centro Universitário Estácio do Ceará, Rua Eliseu Uchoa Becco 600, Fortaleza, CE, CEP 60810-270, Brazil

    Alana de Freitas Pires

  3. Departamento de Farmácia, Odontologia e Enfermagem, Universidade Federal do Ceará, Rua Alexandre Baraúna 949, Fortaleza, CE, CEP 60430-170, Brazil

    Mário Rogério Lima Mota & Pedro Henrique Chaves Isaias

  4. Departamento de Bioquímica E Biologia Molecular, Laboratório de Moléculas Biologicamente Ativas (BioMol-Lab), Universidade Federal do Ceará, Campus do Pici, s/n; Bloco 907, Fortaleza, CE, CEP 60440-970, Brazil

    Maria Gleiciane de Queiroz Martins, Cleane Gomes Moreira, João Batista Cajazeiras, Benildo Sousa Cavada & Kyria Santiago do Nascimento

  5. Department of Biochemistry and Molecular Biology, Federal University of Ceará, Mister Hull s/n Bloco 907, Lab. 1075, PO Box 6043, Fortaleza, CE, 60440-970, Brazil

    Kyria Santiago do Nascimento

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  1. Francisco Lucas Faustino do Nascimento
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  2. Alana de Freitas Pires
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  3. Mário Rogério Lima Mota
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  5. Diego Freitas de Araujo
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  6. Maria Gleiciane de Queiroz Martins
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  8. João Batista Cajazeiras
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  9. Benildo Sousa Cavada
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  10. Kyria Santiago do Nascimento
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  11. Ana Maria Sampaio Assreuy
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Correspondence to Kyria Santiago do Nascimento or Ana Maria Sampaio Assreuy.

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do Nascimento, F.L.F., de Freitas Pires, A., Mota, M.R.L. et al. The anti-inflamatory effect of Andira anthelmia lectin in rats involves inhibition of the prostanoid pathway, TNF-α and lectin domain. Mol Biol Rep 49, 8847–8857 (2022). https://doi.org/10.1007/s11033-022-07735-0

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