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Modulation of B Cell Regulatory Molecules CD22 and CD72 in Myasthenia Gravis and Multiple Sclerosis

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Abstract

B cell activation mediated by cluster of differentiation (CD) molecules plays an important role in B cell-related autoimmune diseases. CD22 and CD72 have been demonstrated to act as B cell inhibitory receptors in many autoimmune diseases. Activated B cells are involved in the pathogenesis of myasthenia gravis (MG) by secretion of anti-acetylcholine receptor (AchR) antibodies. However, the roles of CD22 and CD72 on B cells of MG are unknown. In this study, we detected the expression of CD22 and CD72 on B cells of MG, compared to multiple sclerosis (MS) patient controls and healthy controls by flow cytometry and quantitative real-time polymerase transcription chain reaction. Our data demonstrated that aberrant expression of CD72 exists on B cells of MG and MS patients and expression level of CD72 molecule has a significantly negative correlation with anti-AchR antibody levels in MG, which suggests that CD72 may be involved in the pathogenesis of MG and MS. There were no significant differences between study patients (MG, ocular MG, generalized MG, and MS) and healthy controls.

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Abbreviations

MG:

Myasthenia gravis

MS:

Multiple sclerosis

BCR:

B cell receptor

AchR:

Acetylcholine receptor

CSF:

Cerebrospinal fluid

OMG:

Ocular myasthenia gravis

GMG:

Generalized myasthenia gravis

MP-RT-PCR:

Multiplex reverse transcriptase polymerase chain reaction

HC:

Healthy controls

MuSK:

Muscle-specific kinase

CNS:

Central nervous system

RA:

Rheumatoid arthritis

SLE:

Systemic lupus erythematosus

pSS:

Primary Sjögren’s syndrome

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Acknowledgments

The present work was supported by a research grant from the National Natural Science Foundation of China (30971033). We appreciate the support provided by all the colleagues in the study department.

Conflict of Interest

All the authors declared no competing interests.

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Authors and Affiliations

  1. Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan, 410078, China

    Jiayin Lu, Jing Li, Fa-fa Tian & Huan Yang

  2. Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, 21205, USA

    Jiayin Lu

  3. Department of Neurology, Provincial Zhengzhou People’s Hospital, Zhengzhou, 450003, China

    Tai-qing Zhu

  4. Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, Hunan, 410078, China

    Longbo Zhang

  5. Department of Neurology, Affiliated Hospital of Jining Medical College, Jining, 272029, China

    Yuzhong Wang

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  1. Jiayin Lu
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Correspondence to Jing Li.

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Lu, J., Li, J., Zhu, Tq. et al. Modulation of B Cell Regulatory Molecules CD22 and CD72 in Myasthenia Gravis and Multiple Sclerosis. Inflammation 36, 521–528 (2013). https://doi.org/10.1007/s10753-012-9573-z

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  • DOI: https://doi.org/10.1007/s10753-012-9573-z

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