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Effect of Salvia miltiorrhizae on Pulmonary Apoptosis of Rats with Severe Acute Pancreatitis or Obstructive Jaundice

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Abstract

To investigate the effect of apoptosis about Salvia miltiorrhizae injection on the lungs of SAP and OJ rats. Total 288 rats were used for SAP-associated experiments and OJ-associated experiments, respectively. The rats were randomly divided into sham-operated, model control and treated group. According to the difference of time points after operation, the SAP rats in each group were subdivided into 3, 6 and 12 h groups while the OJ rats were divided into 7, 14, 21 and 28 days groups. The pathological changes, expression levels of Bax protein and apoptotic indexes in the lungs of SAP or OJ rats were observed, and the mortality rates of SAP or OJ rats were recorded, respectively. The numbers of dead SAP and OJ rats in treated groups declined. The pathological changes in the lungs of SAP or OJ rats in treated groups were relieved to varying degrees. There was no marked difference in pathological severity scores and the positive staining intensity of Bax protein between treated groups and model control groups (all P > 0.05). Salvia miltiorrhizae has some protective effect on the lungs of rats with SAP or OJ which may be related apoptosis although our results can not find significant difference between treated groups and model control groups.

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References

  1. Zhang, X. P., D. R. Liu, and Y. Shi. 2007. Study progress in therapeutic effects of traditional Chinese medicine monomer in severe acute pancreatitis. J. Zhejiang Univ. Sci. B. 2:147–152 (PMID:17266191).

    Article  CAS  Google Scholar 

  2. Chan, Y. C., and P. S. Leung. 2007. Acute pancreatitis: animal models and recent advances in basic research. Pancreas 1:1–14 (PMID:17198179).

    Article  Google Scholar 

  3. Dimagno, M. J., and E. P. Dimagno. 2007. New advances in acute pancreatitis. Curr. Opin. Gastroenterol. 5:494–501 (PMID:17762554).

    Google Scholar 

  4. Surbatovi, M., K. Jovanovi, S. Radakovi, and N. Filipovi. 2005. Pathophysiological aspects of severe acute pancreatitis-associated lung injury. Srp. Arh. Celok. Lek. 1–2:76–81 (PMID: 16053182).

    Google Scholar 

  5. Zhu, J. M., L. Wang, H. G. Guan, and Y. R. Chen. 2004. Protective effect of growth hormone on intestinal mucosa of rats with obstructive jaundice and its mechanism. Suzhou University Journal of Medical Science. 5:637–639 667. (in Chinese).

    Google Scholar 

  6. Padillo, F. J., A. Cruz, I. Segura-Jimenez, J. Ruiz-Rabelo, M. R. Vazquez-Ezquerra, M. D. Perea-Alvarez, J. Pena, J. Briceno, and J. Muntane. 2007. Anti-TNF-alpha treatment and bile duct drainage restore cellular immunity and prevent tissue injury in experimental obstructive jaundice. Int. J. Immunopathol. Pharmacol. 4:855–860 (PMID:18179761).

    Google Scholar 

  7. Assimakopoulos, S. F., C. D. Scopa, and C. E. Vagianos. 2007. Pathophysiology of increased intestinal permeability in obstructive jaundice. World J. Gastroenterol. 48:6458–6464 (PMID:18161914).

    Article  Google Scholar 

  8. Sewnath, M. E., T. van der Poll, C. J. van Noorden, F. J. ten Kate, and D. J. Gouma. 2004. Cholestatic interleukin-6-deficient mice succumb to endotoxin-induced liver injury and pulmonary inflammation. Am. J. Respir. Crit. Care Med. 3:413–420 (PMID: 14604838).

    Google Scholar 

  9. Zhang, X. P., Z. J. Li, and D. R. Liu. 2006. Progress in research into the mechanism of Radix Salviae miltiorrhizae in treatment of acute pancreatitis. Hepatobiliary Pancreat. Dis. Int. 4:501–504 (PMID: 17085332).

    Google Scholar 

  10. Gao, D., J. Song, J. Hu, J. Lin, L. Zheng, J. Cai, J. Du, and K. Chen. 2005. Angiogenesis promoting effects of Chinese herbal medicine for activating blood circulation to remove stasis on chick embryo chorio-allantoic membrane. Zhongguo Zhong Xi Yi Jie He Za Zhi. 10:912–915 (PMID: 16313116).

    Google Scholar 

  11. Pastorino, J. G., G. Simbula, K. Yamamoto, P. A. Glascott Jr, R. J. Rothman, and J. L. Farber. 1996. The cytotoxicity of tumor necrosis factor depends on induction of the mitochondrial permeability transition. J. Biol. Chem. 47:29792–29798 (PMID: 8939917).

    Google Scholar 

  12. Li, X. G., Q. G. Liu, and C. E. Pan. 2002. Relationship between NO, MDA and ET in liver tissues and liver injury caused by obstructive jaundice and the protective effect of Danshen on liver. J. Xi’an Med. Univ. 2:93 (in Chinese).

    Google Scholar 

  13. Zhang, J. C., Y. Lin, and P. F. Guo. 2002. Protective effect of Salvia miltiorrhizae on the renal injury of rats with obstructive jaundice. Chin. J. Curr. Adv. Gen. Surg. 1:25 (in Chinese).

    Google Scholar 

  14. Werner, J., C. Fernández-del Castillo, J. A. Rivera, N. Kollias, K. B. Lewandrowski, D. W. Rattner, and A. L. Warshaw. 1998. On the protective mechanisms of nitric oxide in acute pancreatitis. Gut. 3:401–407 (PMID: 9863487).

    Article  Google Scholar 

  15. Zhang, T., and T. L. Chen. 2004. Change of Blood Oxygen Free Radical Levels and Effect of Radix Salviae Miltiorrhizae. Chin. J. Surg. Integr. Trad. Western Med. 10:34–36 (in Chinese).

    Google Scholar 

  16. Huang, X. L., S. Y. Liu, G. P. Wang, H. M. Zeng, L. Yang, and Z. Wang. 2006. Changes of endothelin levels and therapeutic effects of Danshen in lung injury induced by severe acute pancreatitis in rats. World Chin. J. Digestol. 9:853–857 (in Chinese).

    Google Scholar 

  17. Zhang, G. X., H. L. Chen, A. X. Gong, and L. Zhang. 2007. Relationship between pancreatic acinar apoptosis and acute pancreatitis and the therapeutic strategy for acute pancreatitis. World Chin. J. Digestol. 10:1115–1120 (in Chinese).

    Google Scholar 

  18. Dirlik, M., M. Caglikulekci, I. Cinel, L. Cinel, L. Tamer, C. Pata, A. Kanik, K. Ocal, Z. Ogetman, and S. Aydin. 2003. The effect of PARS inhibition on ileal histopathology, apoptosis and lipid peroxidation in LPS-induced obstructive jaundice. Pharmacol. Res. 2:139–149 (PMID: 12798666).

    Article  CAS  Google Scholar 

  19. Xiping, Z., W. Dijiong, L. Jianfeng, C. Qihui, Y. Jing, J. Penghui, Y. Meijuan, and Z. Ninni. 2009. Effects of Salvia miltiorrhizae on ICAM-1, TLR4, NF-kappaB and bax proteins expression in multiple organs of rats with severe acute pancreatitis or obstructive jaundice. Inflammation (in press). [PMID: 19434485]

  20. Zhang, X., D. Liu, D. Wu, C. Zhu, J. Ye, K. Wang, L. Peng, and G. Zhuo. 2009. Effect of Salvia miltiorrhizae on the expressions of TLR4 protein in the liver of rats with SAP or OJ. Inflammation 32:151–162 [PMID: 19370406].

    Article  PubMed  CAS  Google Scholar 

  21. Zhang, X. P., G. H. Feng, J. Zhang, Y. Cai, H. Tian, X. F. Zhang, Y. F. Zhou, Z. W. Wang, and K. Y. Wang. 2009. Protective effects of Salvia miltiorrhizae on the hearts of rats with severe acute pancreatits or obstructive jaundice. J. Zhejiang Univ. Sci. B. 10:193–202 [PMID: 19283874].

    Article  PubMed  Google Scholar 

  22. Zhang, X., L. Chen, J. Zhang, H. Tian, X. Zhang, Y. Zhou, Z. Wang, and K. Wang. 2009. Effect of Salvia miltiorrhizae on apoptosis and NF-kappaB p65 expression in the liver of rats with severe acute pancreatitis or obstructive jaundice. J. Gastroenterol. Hepatol. 24:841–852 [PMID: 19067778].

    Article  PubMed  CAS  Google Scholar 

  23. Xiping, Z., C. Yang, W. Dijiong, Z. Jie, Y. Qian, J. Xinge, M. Meili, Y. Qijun, X. Qi, Z. Yifeng, Y. Wenqin, Z. Ningning, and H. Jiao. 2009. Effects of Salvia miltiorrhiza on intercellular adhesion molecule 1 protein expression in the lungs of rats with severe acute pancreatitis or obstructive jaundice. Pancreas 38:309–317 (PMID: 19034056).

    Article  PubMed  Google Scholar 

  24. Zhang, X. P., L. Zhang, Y. Wang, Q. H. Cheng, J. M. Wang, W. Cai, H. P. Shen, and J. Cai. 2007. Study of the protective effects of dexamethasone on multiple organ injury in rats with severe acute pancreatitis. JOP. 4:400–412 (PMID: 17625291).

    Google Scholar 

  25. Shi, C., R. Andersson, X. Zhao, and X. Wang. 2005. Potential role of reactive oxygen species in pancreatitis-associated multiple organ dysfunction. Pancreatology 4–5:492–500 Epub 2005 Jul 13 (PMID: 16020935).

    Article  CAS  Google Scholar 

  26. Paulino, E. C., L. J. de Souza, N. A. Molan, M. C. Machado, and S. Jancar. 2007. Neutrophils from acute pancreatitis patients cause more severe in vitro endothelial damage compared with neutrophils from healthy donors and are differently regulated by endothelins. Pancreas 1:37–41 (PMID: 17575543).

    Article  Google Scholar 

  27. Wang, J. M., Q. Zou, and S. Q. Zou. 2001. Bcl-2 and Bax expressions and apoptosis in rat liver with obstructive jaundice. Shijie Huaren Xiaohua Zazhi. 8:911–914 (in Chinese).

    Google Scholar 

  28. Zhang, X. P., L. Zhang, H. M. Xu, Y. P. Xu, Q. H. Cheng, J. M. Wang, and H. P. Shen. 2008. Application of tissue microarrays to study the influence of dexamethasone on NF-kappaB expression of pancreas in rat with severe acute pancreatitis. Dig. Dis. Sci. 2:571–580 (PMID: 17574527).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of General Surgery, Hangzhou First People’s Hospital, Hangzhou, 310006, Zhejiang Province, China

    Zhang Xiping, Ye Jing, Yang Qijun & Ying Rongcao

  2. Laboratory Animal Research Center, Zhejiang University, Hangzhou, 310058, Zhejiang Province, China

    Fu Jun

  3. Zhejiang University of Traditional Chinese Medicine, Hangzhou, 310053, Zhejiang Province, China

    Wu Chengjun, Ma Meili, Yan Ping & Zhu Feibo

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  1. Zhang Xiping
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  2. Fu Jun
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Correspondence to Ying Rongcao.

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Note: We claim that the review is original and will not have any financial interest in a company or its competitor, and that all authors meet standard for authorship.

Supported by technological foundation project of Traditional Chinese Medicine Science of Zhejiang province (No. 2003C130; No. 2004C142), foundation project for medical science and technology of Zhejiang province (No. 2003B134), grave foundation project for technological and development of Hangzhou (NO. 2003123B19), intensive foundation project for technology of Hangzhou (NO. 2004Z006), foundation project for medical science and technology of Hangzhou (No. 2003A004) and foundation project for technology of Hangzhou (No. 2005224).

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Xiping, Z., Jun, F., Chengjun, W. et al. Effect of Salvia miltiorrhizae on Pulmonary Apoptosis of Rats with Severe Acute Pancreatitis or Obstructive Jaundice. Inflammation 32, 287–295 (2009). https://doi.org/10.1007/s10753-009-9132-4

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